Frogs from the genus Xenopus have long been used as model organisms in basic and biomedical research. These frogs have helped unlock key fundamental developmental and cellular processes that have led to important scientific breakthroughs and have had practical application in embryology, cancer research and regenerative medicine. Xenopus Development is a vital resource on the biology and development of these key model organisms, and will be a great tool to researchers using these frogs in various disciplines of biological science. Xenopus Development is divided into four sections, the first three highlight key processes in Xenopus development from embryo to metamophosis. These sections focus on the cellular processes, organogenesis and embryo development. The final section highlights novel techniques and approaches being used in Xenopus research. Providing thorough and detailed coverage, Xenopus Development, will be a timely and welcome volume for those working in cell and molecular biology, genetics, developmental biology and biomedical research. Provides broad overview of the developmental biology of both Xenopus laevis and Xenopus tropicalis Explores cellular to systems development in key biomedical model organisms Timely synthesis of the field of Xenopus biology Highlights key biomedical and basic biological findings unlocked by Xenopus

Amphibian embryos are supremely valuable in studies of early vertebrate development because they are large, handle easily, and can be obtained at many interesting stages. And of all the amphibians available for study, the most valuable is Xenopus laevis,which is easy to keep and ovulates at any time of year in response to simple hormone injections. Xenopusembryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopusdevelopment has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation. This manual contains a comprehensive collection of protocols for the study of early development in Xenopusembryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere. This volume is essential reading for all investigators interested in the developmental and cell biology of Xenopusand vertebrates generally. Many of the techniques described here are illustrated in an accompanying set of videotapeswhich are cross-referenced to the appropriate section of the manual.

Developmental biology attracts scientists from many different areas of biology, and the amphibian Xenopus holds a special place among the organisms studied as a model of vertebrate development. Until now, no recently published atlas existed to aid researchers and students coming to the Xenopus embryo for the first time. The present book satisfies this need. With its synthesizing approach and its generous provision of beautiful scanning, transmission, and light microscopy images, this unique volume will be a standard reference not only for developmental biologists but for all students of embryology, histology, and comparative anatomy.

本书的实验方案涵盖了爪蟾胚胎、成体的形态发生,爪蟾中基因表达操作,胚胎的获取和实验常规操作等内容。

The Color Atlas of "Xenopus laevis" Histology provides the first central source on the microscopic anatomy of cells, tissues, and major organs of the adult South African clawed frog, Xenopus laevis. For many years, X. laevis has been a highly popular experimental animal model in many areas of research. The recent development of transgenic Xenopus technology offers the promise that this animal model will be utilized more than ever before. The purpose of this book is to provide the active researcher with a central source of high quality light microscopic color images of the tissues of X. laevis, to aid in the identification of the cells and tissues of interest.

This volume presents the first comprehensive treatment of the wide range of uses for Xenopus laevis oocytes and embryos in cell and molecular biology. Each chapter includes background information, experimental protocols, and suggested applications. An extensive array of techniques is featured. The authors are experienced researchers who have written chapters that will be useful to both experienced researchers and to those new to Xenopus as an experimental system. Full-color plates and diagrams enhance the educational value of this book, which provides a valuable permanent resource for all laboratories that use Xenopus.* Features approximately twenty full-color plates illustrating experimental techniques and results and depicting embryonic development* Provides complete coverage of Xenopus laevis as an experimental system including* Embryonic development, genetics, and laboratory care* Up-to-date protocols for experimental techniques using oocytes and embryos* General information listing recipes, suppliers, sequences, codons, and clones