In the nineteenth century, scientific practice underwent a dramatic transformation from personal endeavor to business enterprise. In Spectrum of Belief, Myles Jackson explores this transformation through a sociocultural history of the rise of precision optics in Germany. He uses the career of the optician Joseph von Fraunhofer (1787-1826) to probe the relationship between science and society, and between artisans and experimental natural philosophers, during this important transition. Fraunhofer came from a long line of glassmakers. Orphaned at age eleven, the young apprentice moved in with his master, the court decorative glass cutter. At age nineteen, bored with his work and angered by his master's refusal to allow him to study optical theory, Fraunhofer took a position at the Optical Institute assisting in the manufacture of achromatic lenses. Within ten years he was producing the world's finest achromatic lenses and prisms. Housed in an old Benedictine monastery, Fraunhofer's laboratory mirrored the labor of the monks. Because of his secrecy (after his death, even those who had worked most closely with him could not achieve his success), British experimental natural philosophers were unable to reproduce his work. This secrecy, while guaranteeing his institute's monopoly, thwarted Fraunhofer's attempts to gain credibility within the scientific community, which looked down on artisanal work and its clandestine practices as an affront. The response to the ensuing rise of German optical technology sheds light on crucial social, economic, and political issues of the period, such as mechanization, patent law reform, the role of skills in both physics and society, the rise of Mechanics' Institutes, and scientific patronage. After his death, Fraunhofer's example was used in the newly united Germany to argue for the merging of scientific research and technological innovation with industrial and state support.

This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike.

PROBLEM. The treatise is devoted to the reconstruction of our 'instinctive beliefs' in classical mechanics and to present them 'as much isolated and as free from irrelevant additions as possible'. The same motivation has driven many authors since the publication of Newton's Principia. IMPORTANCE. Classical mechanics will remain the basic reference and tool for mechanics on terrestrial and planetary scale as well as the proto-theory of relativistic and quantum mechanics. But it can only serve its purpose if it is not considered as obsolete, but if its foundations and implications are understood and made 'absolutely' clear. METHOD. Based on the 'instinctive belief' that the foundations of classical mechanics cannot be found and reconstructed within mechanics itself but only 'outside', classical mechanics is 'understood' by embedding it into an adequate theory of knowledge and adequate proto- and meta-theories in terms of the 'language of dynamics'. Evidence is produced that available philosophical expositions are not adequate for the purpose at hand. Mechanics is treated as part of physics, not of mathematics. Not sophisticated mathematical artifacts, necessary for solving specific problems, but the intellectually satisfactory foundation of mechanics in general is subject and purpose of the exercise. The goal is reached using axiomatic systems as models. SCOPE. Following an account of the unsatisfactory state of affairs the treatise covers the epistemological foundations, abstract proto-mechanics, i. e. the theories of time and space, meta-mechanics, i. e. the theories of state space models and of quantities proper, and, as an instance of the latter, abstract elementary mechanics, the theory of translational motions of 'small' solid bodies in three-dimensional Euclidean space, including classical general relativity. Subsequently the theory of classical kinematics is developed as basis for interpreted proto-mechanics and interpreted elementary mechanics. As an amus