This book presents emerging contemporary optical techniques of ultrafast science which have opened entirely new vistas for probing biological entities and processes. The spectrum reaches from time-resolved imaging and multiphoton microscopy to cancer therapy and studies of DNA damage. The book displays interdisciplinary research at the interface of physics and biology. Emerging topics on the horizon are also discussed, like the use of squeezed light, frequency combs and terahertz imaging as the possibility of mimicking biological systems. The book is written in a manner to make it readily accessible to researchers, postgraduate biologists, chemists, engineers, and physicists and students of optics, biomedical optics, photonics and biotechnology.

Biophotonics involves understanding how light interacts with biological matter, from molecules and cells, to tissues and even whole organisms. Light can be used to probe biomolecular events, such as gene expression and protein-protein interaction, with impressively high sensitivity and specificity. The spatial and temporal distribution of biochemic

Advanced photonics methods for biomedical applications give researchers in universities and industries, and clinicians an overview of the novel tools for cancer diagnostics and treatment. This book provides researchers and professionals in the area of biomedical photonics with a toolbox of novel methodologies for biomedical applications, including health diagnostics, cancer detection, and treatment. It covers the theory, modeling, and design of each method, alongside their applications, fabrication, characterization, and measurements in clinical practice. A wide scope of concepts concerning innovative science and technologies of medicine will be covered, providing the readers with the latest research, developments, and technologies. It will also be a valuable resource for students and early-career researchers, alongside those involved in the design of the novel photonics-based techniques for health diagnostics and cancer detection and treatment. Key features • Discusses novel methods of cancer diagnostics and cancer treatment. • Details non and minimally invasive photonics techniques. • Explores the applications of machine learning and artificial intelligence to these novel techniques.

Ellipsometry is rapidly emerging as a popular solution addressed to new materials science challenges and technological pitfalls hindering its effective application on modern problems. Amid the nowadays active development of materials of top notch, ellipsometry is also evolving rapidly both in the academic and industry sectors. The global industry strategies, introduce the latest scientific advances at manufacturing new, more accurate, and reliable ellipsometry systems to tackle emerging challenges. The book provides a comprehensive overview on the principles and technical capabilities of the modern ellipsometry highlighting its versatility in materials characterization.

Written by a team of European experts in the field, this book addresses the physics, the principles, the engineering methods, and the latest developments of efficient and compact ultrafast lasers based on novel quantum-dot structures and devices, as well as their applications in biophotonics. Recommended reading for physicists, engineers, students and lecturers in the fields of photonics, optics, laser physics, optoelectronics, and biophotonics.

This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence lifetime imaging The long journey to the laser and its use for nonlinear optics Advanced TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II: Modern nonlinear microscopy of live cells STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities Principles and applications of temporal-focusing wide-field two-photon microscopy FLIM-FRET microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing Laser tweezers are sources of two-photon effects Metabolic shifts in cell proliferation and differentiation Femtosecond laser nanoprocessing Cryomultiphoton imaging Part III: Nonlinear tissue imaging Multiphoton Tomography (MPT) Clinical multimodal CARS imaging In vivo multiphoton microscopy of human skin Two-photon microscopy and fluorescence lifetime imaging of the cornea Multiscale correlative imaging of the brain Revealing interaction of dyes and nanomaterials by multiphoton imaging Multiphoton FLIM in cosmetic clinical research Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models Bedside assessment of multiphoton tomography

This book, now in its fourth edition, is a well-known classic on the ultrafast nonlinear and linear processes responsible for supercontinuum generation. The book begins with chapters reviewing the experimental and theoretical understanding of the field along with key applications developed since the discovery of the supercontinuum effect. The chapters that follow cover recent research activity on supercontinuum phenomena, novel applications, and advances achieved since the publication of the previous edition. The new chapters focus on: filamentation in gases, air, and condensed media; conical emission by four-wave mixing and X-waves; electronic self-phase mechanism; higher harmonics generation; attosecond laser pulses; complex vector beam supercontinuum; higher order self-phase modulation and cross-phase modulation; nonlinear supercontinuum interference in uniaxial crystals; new nonlinear microscopes involving supercontinuum and ultrafast lasers with biomedical applications; and other current supercontinuum applications in communications. The Supercontinuum Laser Source is a definitive work by one of the discoverers of the white light effect. It is indispensable reading for any researcher or student working in the field of ultrafast laser physics. Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.