Transgenic Stem Cells for Craniofacial Bone Reconstruction
Title | Transgenic Stem Cells for Craniofacial Bone Reconstruction PDF eBook |
Author | Jin Ke |
Publisher | |
Pages | |
Release | 2017-01-26 |
Genre | |
ISBN | 9781361247976 |
This dissertation, "Transgenic Stem Cells for Craniofacial Bone Reconstruction" by Jin, Ke, 柯金, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Bone loss from the cranio-maxillofacial region is a major clinical problem affecting patients worldwide. Conventional treatment strategy includes the use of autogenous or allogeneic bone, biomaterials, and osteogenic growth factors. However, there has been no effective therapy for most cases so far. Stem cell-based gene therapy is the latest research method with possible applications in humans. The present study aims to (1) characterize rabbit mesenchymal stem cells (MSCs) relating to growth pattern, surface antigens, and the potential for multi-differentiation; (2) determine the transduction efficiency and duration of recombinant adeno-associated virus2 carrying enhanced green fluorescent protein (rAAV2EGFP) reporter gene in rabbit MSCs and study the effects of rAAV2EGFP transduction on stem cells' phenotype and capacity of multi-differentiation; (3) evaluate the differentiation characteristics of rabbit MSCs following recombinant adeno-associated virus 2 carrying bone morphogenetic protein 2 gene (rAAV2BMP2) transduction; (4) investigate whether MSCs transduced by rAAV2BMP2 could successfully induce bone regeneration in rabbit critical-size cranial defects. MSCs were isolated from bone marrows of rabbit tibias and cultured. Cell counting and colony-forming assays demonstrated that growth rates of MSCs dropped substantially with increasing passages. Flow cytometry on MSCs at passage 1 showed that cells expressed high level of CD49a and low level of CD44 as well as stage-specific embryonic antigen 4 (SSEA4). Multi-differentiation and reverse transcriptase-polymerase chain reaction (RTPCR) tests demonstrated that rabbit MSCs were capable to differentiate into osteocytes, chondrocytes and adipocytes. Immunofluorescence microscopy showed that rabbit MSCs produced a series of hematopoietic growth factors, including stem cell factor (SCF), vascular endothelial growth factor-A (VEGFA) and granulocyte macrophage colony-stimulating factor (GMCSF). Subsequently, rabbit MSCs were transduced with rAAV2EGFP in vitro. By comparing the transduction efficiency with different doses of rAAV2EGFP particles, multiplicity of infection (MOI) of 1 x 10 4 was identified as an optimal parameter for the transduction of rAAV2 in rabbit MSCs. Fluorescent microscopy demonstrated long-term expression of EGFP in rabbit MSCs after transduction both in vitro and in vivo. In addition, cell proliferation assay, adipogenic induction test and flow cytometry showed that rAAV2EGFP transduced MSCs exhibited a similar pattern with non-transduced cells on the cell growth, capacity of adipogenic differentiation and expression of surface antigens, indicating that rabbit MSCs maintain their stem cell properties after rAAV2EGFP transduction. DOI: 10.5353/th_b4436297 Subjects: Facial bones - Surgery Bone regeneration Stem cells - Therapeutic use Transgenic animals - Research
Transgenic Stem Cells for Craniofacial Bone Reconstruction
Title | Transgenic Stem Cells for Craniofacial Bone Reconstruction PDF eBook |
Author | Jin Ke |
Publisher | |
Pages | 316 |
Release | 2010 |
Genre | Bone regeneration |
ISBN |
Stem Cells in Craniofacial Development and Regeneration
Title | Stem Cells in Craniofacial Development and Regeneration PDF eBook |
Author | George T.J. Huang |
Publisher | John Wiley & Sons |
Pages | 578 |
Release | 2013-02-15 |
Genre | Science |
ISBN | 1118498119 |
Stem Cells, Craniofacial Development and Regeneration is an introduction to stem cells with an emphasis on their role in craniofacial development. Divided into five sections, chapters build from basic introductory information on the definition and characteristics of stem cells to more indepth explorations of their role in craniofacial development. Section I covers embryonic and adult stem cells with a focus on the craniofacial region, while sections II-IV cover the development and regeneration of craniofacial bone, tooth, temporomandibular joint, salivary glands and muscle. Concluding chapters describe the current, cutting-edge research utilizing stem cells for craniofacial tissue bioengineering to treat lost or damaged tissue. The authoritative resource for dentistry students as well as craniofacial researchers at the graduate and post-graduate level, Stem Cells, Craniofacial Development and Regeneration explores the rapidly expanding field of stem cells and regeneration from the perspective of the dentistry and craniofacial community, and points the way forward in areas of tissue bioengineering and craniofacial stem cell therapies.
Mesenchymal Stem Cells and Craniofacial Regeneration
Title | Mesenchymal Stem Cells and Craniofacial Regeneration PDF eBook |
Author | Jing Wang |
Publisher | Bentham Science Publishers |
Pages | 408 |
Release | 2016-10-19 |
Genre | Science |
ISBN | 1681083159 |
This monograph provides a current and in-depth review of scholarly information about mesenchymal stem cells and their application in the craniofacial region of the human body. Chapters in this volume cover biological and conceptual information about mesenchymal stem cells, induced pluripotent stem cells, craniofacial regeneration, new methods of scaffold fabrication, tooth regeneration and three-dimensional printing in dentistry. The book is suitable for clinicians and cell biologists aiming to gain a better understanding of the promising field of craniofacial regenerative medicine.
Anabolic Treatments for Osteoporosis
Title | Anabolic Treatments for Osteoporosis PDF eBook |
Author | James F. Whitfield |
Publisher | CRC Press |
Pages | 281 |
Release | 2013-11-12 |
Genre | Medical |
ISBN | 1482234033 |
Osteoporosis, a growing epidemic among women in North America, Europe, and Japan, is a painful, costly disease that has presented a treatment challenge to healthcare professionals. Until recently, therapies have focused on agents that slow bone resorption, and have had only limited success at increasing bone mass.
Bone Tissue Engineering
Title | Bone Tissue Engineering PDF eBook |
Author | Jeffrey O. Hollinger |
Publisher | CRC Press |
Pages | 500 |
Release | 2004-10-14 |
Genre | Medical |
ISBN | 1135501912 |
Focusing on bone biology, Bone Tissue Engineering integrates basic sciences with tissue engineering. It includes contributions from world-renowned researchers and clinicians who discuss key topics such as different models and approaches to bone tissue engineering, as well as exciting clinical applications for patients. Divided into four sections, t
Neural Crest Stem Cells
Title | Neural Crest Stem Cells PDF eBook |
Author | Maya Sieber-Blum |
Publisher | World Scientific |
Pages | 169 |
Release | 2012 |
Genre | Medical |
ISBN | 9814343803 |
Offers readers an understanding of the development of neural crest cells, which is crucial as many birth defects and tumours are of neural crest origin. Delving into stem cells from different locations of the body, this book explores the best possible source of such cells for the use in medical applications.