This book reviews the theoretical concepts and experimental details underpinning the broad range of modern technologies that are currently being used to advance our understanding of the biomolecular sciences.

If you are studying the biomolecular sciences - including biochemistry, biomedical sciences, biotechnology, genetics, microbiology and molecular biology - then this book will be an indispensable companion throughout the whole of your degree programme. It provides effective explanation and support for the development of a wide range of laboratory and data analysis skills that you will use time and again during the practical aspects of your studies. This book also gives you a solid grounding in the broader transferable skills, which are increasingly necessary to achieve a high level of academic success.

If you are a studying within the biomolecular sciences (including biochemistry, biomedical sciences, biotechnology, genetics, microbiology and molecular biology) then this book will be an indispensable companion throughout the whole of your degree programme. It provides effective support for the development of the laboratory and data analysis skills that you will draw on time and again for the practical aspects of your studies, and also gives you a solid grounding in the broader transferable skills which are increasingly necessary to achieve a high level of academic success. Now in its fourth edition, Practical Skills in Biomolecular Sciences has been enhanced and updated throughout to provide a complete and easy-to-read guide to developing all of the skills you need from your first day through to graduation, establishing its reputation as the practical resource for students of the biomolecular sciences.

Thousands of methods have been developed in the various biomedical disciplines, and those covered in this book represent the basic, essential and most widely used methods in several different disciplines.

An accessible overview of the most popular and cutting-edge methods for studying the properties of molecules and their interactions.

Alternative techniques and tools for analyzing biomolecular networks With the recent rapid advances in molecular biology, high-throughput experimental methods have resulted in enormous amounts of data that can be used to study biomolecular networks in living organisms. With this development has come recognition of the fact that a complicated living organism cannot be fully understood by merely analyzing individual components. Rather, it is the interactions of components or biomolecular networks that are ultimately responsible for an organism's form and function. This book addresses the important need for a new set of computational tools to reveal essential biological mechanisms from a systems biology approach. Readers will get comprehensive coverage of analyzing biomolecular networks in cellular systems based on available experimental data with an emphasis on the aspects of network, system, integration, and engineering. Each topic is treated in depth with specific biological problems and novel computational methods: GENE NETWORKS—Transcriptional regulation; reconstruction of gene regulatory networks; and inference of transcriptional regulatory networks PROTEIN INTERACTION NETWORKS—Prediction of protein-protein interactions; topological structure of biomolecular networks; alignment of biomolecular networks; and network-based prediction of protein function METABOLIC NETWORKS AND SIGNALING NETWORKS—Analysis, reconstruction, and applications of metabolic networks; modeling and inference of signaling networks; and other topics and new trends In addition to theoretical results and methods, many computational software tools are referenced and available from the authors' Web sites. Biomolecular Networks is an indispensable reference for researchers and graduate students in bioinformatics, computational biology, systems biology, computer science, and applied mathematics.