"Abstract: We present novel rearing records of Agromyzidae (Diptera) from throughout the United States. We describe leaf mines or other larval habits for 27 species, plus five others whose identification is tentative, and another five that are identified only to genus due to the absence of male specimens. We review host and distribution data for the known species, reporting 26 new host species records (including the first rearing records for Phytomyza flexuosa Spencer, P. notopleuralis Spencer, and (tentatively identified) Ophiomyia frosti Spencer) and 25 new state records (including the first USA records for P. krygeri Hering, P. thermarum (Griffiths), and (tentatively identified) Liriomyza cracentis Lonsdale). We also describe and provide natural history information for the following 13 new species: Haplopeodes loprestii Eiseman & Lonsdale, Liriomyza euphorbivora Eiseman & Lonsdale, L. hypopolymnia Eiseman & Lonsdale, Melanagromyza arnoglossi Eiseman & Lonsdale, M. gentianivora Eiseman & Lonsdale, M. hieracii Eiseman & Lonsdale, M. rudbeckiae Eiseman & Lonsdale, M. urticae Eiseman & Lonsdale, M. verbenivora Eiseman & Lonsdale, Ophiomyia nabali Eiseman & Lonsdale, O. rugula Eiseman & Lonsdale, Phytomyza flavilonicera Eiseman & Lonsdale, and P. triostevena Eiseman & Lonsdale. Keywords: leafminer, plant-insect interactions, rearing"--Page 4.