This book is the first comprehensive compilation of current knowledge on mulberry (Morus L.) covering botany, cytogenetics, biodiversity, genetics and breeding, tissue culture and genetic transformation, biotic and abiotic stresses, molecular mapping, QTL identification, whole genome sequencing and elucidation on functional genomics. As mulberry is one of the most economically important trees in Asian countries, it has attracted the attention of both academicians as well as industrialists. Being highly heterozygous due to long juvenile life coupled cross pollination among species, the genetics of this important crop species is yet to be unravelled. Nonetheless, the recent success of sequencing the genomes of haploid and diploid domesticated species has ushered in an era of intense molecular and genetic research to understand this crop well for its better utilization for mankind. In this book, efforts have been made to bring together key information on origin and distribution, taxonomy, morphological features, economic importance, abiotic stress responses, disease and pest resilience, current breeding strategies and their constraints, progress and prospects of gene mapping, elucidation of genes controlling metabolic and physiological pathways, and their utilization in crop improvement which are elaborated in about 250 pages over 13 chapters authored by globally leading experts on the species presented. This book is useful to the sericulture community in the world in general and students, teachers, and scientists in the academia for forage and fruit production, genetics, breeding, pathology, entomology, physiology, molecular genetics, in vitro culture and genetic engineering, and structural and functional genomics. This book is also useful to seed and biofuel industries.

Mulberry (Morus spp.) is an important horticultural plant in the sericulture industry. It belongs to the family Moraceae. The leaf of mulberry is used to feed the silkworm Bombyx mori L. It is also used as a fodder. Due to its economic and agricultural importance, mulberry is cultivated in many parts of the world. An estimated 60% of the total cost of silk cocoon production is for production and maintenance of mulberry plants. Therefore, much attention is needed to improve the quality and quantity of mulberry leaves. It is vital to increase the production of superior quality mulberry leaves with high nutritive value for the sericulture industry. Although a lot of research is going on in mulberry, very little effort has been made to compile the results of this research in a single book. This book provides an update of recent research works going on in this plant. It describes the taxonomy, conservation of germplasm, genetic diversity of various mulberry species, application of breeding techniques to improve the quality of mulberry, in vitro conservation, application of tissue culture techniques to improve mulberry species, production of haploids and triploids in mulberry and improvement of abiotic stress adaptive traits in mulberry with relevance to adaptiveness to global warming.

The genesis of the volume, Plant Biotechnology and Molecular Markers, has been the occasion of the retirement of Professor Sant Saran Bhojwani from the Department of Botany, University of Delhi. For Professor Bhojwani, retirement only means relinquishing the chair as being a researcher and a teacher which has always been a way of life to him. Professor Bhojwani has been an ardent practitioner of modern plant biology and areas like Plant Biotechnology and Molecular Breeding have been close to his heart. The book contains original as well as review articles contributed by his admirers and associates who are experts in their area of research. While planning this contributory book our endeavour has been to incorporate articles that cover the entire gamut of Plant Biotechnology, and also applications of Molecular Markers. Besides articles on in vitro fertilization and micropropagation, there are articles on forest tree improvement through genetic engineering. Considering the importance of conservation of our precious natural wealth, one article deals with cryopreservation of plant material. Chapter on molecular marker considers DNA indexing as markers of clonal fidelity of in vitro regenerated plants and prevention against bio-piracy. A couple of write-ups also cover stage-specific gene markers, DNA polymorphism and genetic engineering, including raising of stress tolerant plants to sustain productivity and help in reclamation of degraded land.

Mulberry (Morus spp.) is widely distributed tree taxon found almost in every continent across the globe. Habitat of this plant species is very much diversified, as it is found across all climatic zones ranging from tropical, sub-tropical, temperate, tundra, semi-arid to desert (arid) conditions. It flourishes on all types of landforms; mountains, valleys, plateaus, forests, grasslands, hills, plains and arid lands. Successful utilization of mulberry leaf for silkworm rearing and production of quality cocoons has been studied at length. Now, mulberry is being recognized as a multipurpose plant by most of the countries across the globe. As mulberry is eco-friendly in nature, the propagation of mulberry needs to be done at large scale to address the ecological issues like conversion of arid lands to cultivable lands, eco-restoration of degraded lands, bioremediation of polluted land sites, conservation of water and soil, cleaning the air pollution in urban areas, utilization of mulberry in producing renewable energy in the form of biodiesel. Similarly, there is the need to produce the identified and highly commercially valued pharmaceutical compounds of mulberry under laboratory conditions through in vitro culture based secondary metabolite production through enhanced expression under the stress conditions or in presence of elicitors. Lately protocols have been developed for genetic transformation of mulberry through agrobacterium mediated and particle bombardment mediated gene transfer techniques. Biotechnology based molecular breeding techniques could also be utilized in raising the improved lines through marker assisted selection, soma clonal variations, mutational breeding, somatic hybridization, genome editing and other genetic engineering approaches. Apart from sericulture; mulberry should also be utilized and exploited in other sectors across the globe for additional revenue generation, for livestock maintenance, for environmental safety and in promoting human health. These diversified aspects of mulberry coupled with its economic importance in revenue generation through sericulture, animal husbandry and industrial products has prompted us to prepare this book. It will present a comprehensive account of mulberry plant under 9 chapter headings: introduction, botanical features, ecological features, ecophysiological aspects, interactions and development, molecular aspects, propagation and production, economic importance, and global perspectives as well as future approaches.

This book provides updated and all-inclusive data and evidences for Moringa botany, cytogenetical analysis, genetic resources and diversity, classical genetics, traditional breeding, tissue culture, genetic transformation, whole-genome sequencing, comparative genomics and elucidation on applications of functional genomics, nanotechnology, bioinformatics, processing and value addition besides providing perspectives of medicinal and therapeutic properties of Moringa. Moringa gained global attention in the recent past owing to its unique blend of affordable nutraceutical and pharmaceutical compounds in all parts of the plants. Scientific literatures supporting its health benefits besides the studies on its utility in various fields are scattered on several reports. This book is written by renowned global subject experts by compiling and narrating it in a sober style.

This book presents deliberations on molecular and genomic mechanisms underlying the interactions of crop plants to the abiotic stresses caused by heat, cold, drought, flooding, submergence, salinity, acidity, etc., important to develop resistant crop varieties. Knowledge on the advanced genetic and genomic crop improvement strategies including molecular breeding, transgenics, genomic-assisted breeding, and the recently emerging genome editing for developing resistant varieties in technical crops is imperative for addressing FHNEE (food, health, nutrition, energy, and environment) security. Whole genome sequencing in many of these crops followed by genotyping-by-sequencing has provided precise information regarding the genes conferring resistance useful for gene discovery, allele mining, and shuttle breeding which in turn opened up the scope for 'designing' crop genomes with resistance to abiotic stresses. The ten chapters each dedicated to a technical crop and one chapter devoted to a crop group in this volume elucidate different types of abiotic stresses and their effects on and interaction with the crops; enumerate the available genetic diversity with regard to abiotic stress resistance among available cultivars; illuminate the potential gene pools for utilization in interspecific gene transfer; present brief on classical genetics of stress resistance and traditional breeding for transferring them to their cultivated counterparts; depict the success stories of genetic engineering for developing abiotic stress-resistant crop varieties; discuss on molecular mapping of genes and QTLs underlying stress resistance and their marker-assisted introgression into elite varieties; enunciate different genomics-aided techniques including genomic selection, allele mining, gene discovery, and gene pyramiding for developing adaptive crop varieties with higher quantity and quality of yields, and also elaborate some case studies on genome editing focusing on specific genes for generating abiotic stress-resistant crops.

Biotic stresses cause yield loss of 31-42% in crops in addition to 6-20% during post-harvest stage. Understanding interaction of crop plants to the biotic stresses caused by insects, bacteria, fungi, viruses, and oomycetes, etc. is important to develop resistant crop varieties. Knowledge on the advanced genetic and genomic crop improvement strategies including molecular breeding, transgenics, genomic-assisted breeding and the recently emerging genome editing for developing resistant varieties in technical crops is imperative for addressing FHEE (food, health, energy and environment) security. Whole genome sequencing of these crops followed by genotyping-by-sequencing have facilitated precise information about the genes conferring resistance useful for gene discovery, allele mining and shuttle breeding which in turn opened up the scope for 'designing' crop genomes with resistance to biotic stresses. The 15 chapters dedicated to 13 technical crops and 2 technical crop groups in this volume will deliberate on different types of biotic stress agents and their effects on and interaction with crop plants; will enumerate on the available genetic diversity with regard to biotic stress resistance among available cultivars; illuminate on the potential gene pools for utilization in interspecific gene transfer; will brief on the classical genetics of stress resistance and traditional breeding for transferring them to their cultivated counterparts; will enunciate the success stories of genetic engineering for developing biotic stress resistant varieties; will discuss on molecular mapping of genes and QTLs underlying biotic stress resistance and their marker-assisted introgression into elite varieties; will enunciate on different emerging genomics-aided techniques including genomic selection, allele mining, gene discovery and gene pyramiding for developing resistant crop varieties with higher quantity and quality; and will also elaborate some case studies on genome editing focusing on specific genes for generating disease and insect resistant crops.