The Cell Nucleus, Volume III focuses mainly on nucleic acids, nuclear proteins, and special aspects of nuclear functions. This volume particularly discusses the organization of bacterial and viral DNA, as well as the nuclear DNA of eukaryotic organisms. It also describes nuclear DNA polymerases, precursors of messenger RNA, ribonucleoproteins, and nuclear high- and low-molecular-weight RNAs. Furthermore, this volume looks into the two broad classes of nuclear proteins: histones and nonhistone proteins. It also presents advances made in the knowledge of mammalian DNA-dependent RNA polymerase, cytochemical detection of nuclear enzymes, and nuclear protein synthesis. Moreover, it elucidates the effects of female steroid hormones on target cell nuclei, describes the nucleus during avian erythropoiesis, and reports the general properties of intranuclear viruses.

Biochemical Actions of Hormones, Volume III is a collection of papers that deals with steroid hormone action, hypothalamic-regulating hormones, plasma membrane receptors, thyroid hormones, hormones acting on the synthesis of proteins in liver perfusion systems, as well as on approaches using genetics and cell culture. One paper explains why cell hybridization can be a useful technique in studying both genetic control of differentiated functions and of hormonal induction. Another paper discusses the general approaches in the study of ligand-membrane interactions, and cites experiments dealing with polypeptide hormones and catecholamines. It explains in detail the physiochemical interaction between a radioactively labeled ligand and the plasma membrane, either as found in an intact cell or in an isolated membrane preparation. One paper discusses the introduction and time course of estrogen-stimulated biosynthetic events in the uterus. It analyzes the relationship of the estrogen-binding protein to the biological responses of the uterus, including the "domino" versus "sustained output" model of estrogen action. One paper explains, by using a chick oviduct, how to investigate the hypothesis that hormones can activate genes to allow transcription of new species of messenger RNA. This collection can prove beneficial to biochemists, molecular biologists, cellular biologists, micro-biologists, developmental biologists, and scientists involved in cell research.

Cell Biology, A Comprehensive Treatise, Volume 3: Gene Expression: The Production of RNA's mainly discusses the molecular and cytological bases of gene expression. The coverage begins with the concepts of organization of DNA and gene sequences in chromosomes, as an introduction to a more detailed coverage of gene expression. The book opens with a general discussion on the organization of DNA sequences in chromosomes. This chapter includes different methods of analyzing DNA sequences. As the book progresses, it looks upon the details on gene reiteration and amplification up to the transcription of prokaryotes and eukaryotes. It includes the ways of regulating transcription. The following chapters deal mostly with the structure and activity of genes up to the different virus strains in both RNA and DNA. The cytoplasmic and environmental impact on gene expression is also discussed. Chapter 8 generally tackles the DNA conformation and template function. The succeeding chapters focus on the transfer and ribosomal RNA as a result of maturation events; the processing of hnRNA and its relation to mRNA; and recombinant DNA procedures. The book closes with the directory of the different classes of cellular RNAs. This book will be helpful to many graduate students, teachers, scientists, and researchers in need of information regarding cell biology.

Growth, Nutrition, and Metabolism of Cells in Culture, Volume 3, focuses on a number of specific, timely areas of research that make use of cell and tissue culture. The major theme of this volume is growth and its regulation in animal cells. The book includes studies on the role of growth factors in cell culture systems; the effects of cyclic nucleotides in cell proliferation in culture; metabolic regulation during the cell cycle; and the role of the cell surface in growth and metabolic regulation. There are also separate chapters on aspects of abnormal cell growth and metabolism; DNA repair; genetic analysis using cell fusion techniques; the growth of vascular cells in culture for atherosclerosis research; the culture of haploid vertebrate cells for genetic analysis of cell function; data on haploid cell culture; and the value of using cell cultures to test for the possible toxicity of various pharmacologic agents.

Metabolic Inhibitors: A Comprehensive Treatise, Volume III reviews developments in metabolic and enzyme inhibition. With contributions by investigators experienced in their respective fields, the book explores metabolic processes or systems and covers topics ranging from membrane transport to immunization; gene activity; DNA, RNA, and protein syntheses; photosynthesis; lipid metabolism; and blood clotting. Organized into 12 chapters, this volume begins with an overview of transport reactions and their inhibition, emphasizing inhibitors of ATPase including cations, substrates, and products. Some chapters deal with inhibitors, such as antibiotics; polypeptide and protein hormones; modified transfer RNAs; and oligonucleotides. Other chapters discuss inhibitors of immune reactions; animal virus replication; plant viruses and mycoplasma; and isozymes. An account of genetic deletions is also given. Finally, the book considers molecules that act as repressors and derepressors of gene activity. This book will be beneficial to biochemists and medical research workers, as well as to virologists, microbiologists, plant physiologists, and agronomists.

Isozymes, III: Developmental Biology contains manuscripts presented at the Third International Conference on isozymes convened in April 1974 at Yale University. Separating 61 manuscripts into chapters, this book begins by discussing the areas of ""isozymology"" that appear particularly promising for further developmental analysis. This text also looks into the role of isozymes as genetic markers in early mammalian development, as well as the mechanisms of intracellular enzyme localization. Significant topics on specific isozymes are given separately in other chapters.

The green alga Chlamydomonas is widely used as an experimental model system for studies in cellular and molecular biology, and in particular plant molecular biology. This book is the only single modern compendium of information on its biology and in particular its molecular biology and genetics. Included in addition to much information on the basic biology is material of a very practical nature, namely, methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants. Casual users as well as specialists will find the book to be useful in many ways. - Provides access to previously unpublished data from genetic analysis - Provides descriptions of mutant strains - Depicts summary tables comparing properties of different species and their mutant strains - Explains detailed methods for laboratory procedures of general utility - Furnishes comparisons of culture media - Presents lists of inhibitors, mutagens, and other additives to culture media - Assists with common laboratory problems such as contamination and storage of strains - Demonstrates protocols for laboratory demonstrations available for undergraduate teaching.