La biologie moléculaire a bouleversé les sciences du vivant L'explosion de la génomique, qui propose des séquences de génomes entiers ainsi que des approches globales de leur fonctionnement, en est un exemple récent. L'objectif de cet ouvrage présenté sous forme de fiches n'est pas de détailler des protocoles ou des recettes toutes faites, mais d'expliquer simplement les principes théoriques des techniques de biologie moléculaire. Cette édition mise à jour propose des illustrations nouvelles et présente notamment de nombreuses techniques de génomique récemment apparues dans les laboratoires. Cet ouvrage s'adresse à toute personne - spécialiste ou non - curieuse de connaître les bases des différentes techniques de manipulation des acides nucléiques.

Les techniques de biologie moléculaire sont des méthodes couramment utilisées en biologie moléculaire, biochimie, génétique et biophysique qui impliquent généralement la manipulation et l'analyse de DNA, RNA, des protéines et des lipides. Contenu de ce livre: Biologie moléculaire, Génétique moléculaire, Techniques de génie génétique: Un bref résumé, Outils de génétique moléculaire humaine, Techniques de biologie moléculaire, Affinity capture, Balayage à l'alanine, Oligonucléotide spécifique à un allèle, Amplicon, ATAC-seq, Bio interférométrie en couches, essai DNA ramifié, comptage cellulaire, unité formant colonie, culture cellulaire 3D par lévitation magnétique, culture cellulaire, culture de cellules non mammifères, lignées cellulaires communes, milieu chimiquement défini, Chem-seq, ChIA-PET, ChIL-sequencing, ChIP-exo, ChIP-on-chip, ChIP-sequencing, Immunoprécipitation Chromatine, Chromogène in situ hybridization, COLD-PCR, Colonie hybridization, Analyse de restriction de bisulfite combinée, Community fingerprinting, Competition-ChIP, DNA footprinting, DNA microarray, DNA séquençage, séquençage parallèle massif, DNA brassage, DNA Spécimen Provenance Assignment, DNase-Seq, Dot blot, DRIP-seq, Eastern Blot, EHA101, End-sequence profilage, Exome sequencing, test d' extension Poly(A), FAIRE-Seq, Far-eastern blot, Far-western blot, Protéolyse parallèle rapide, glucide assisté par fluorophore electrophoresis, transfert d'énergie par résonance de Förster, fonction-espaceur-lipide Construction Kode, Gel doc

With the underpinning role of forage legumes in the nitrogen economy and animal productivity from temperate grasslands certain to expand in the future, particularly in regions where their potential has not yet been realized, it is essential that the wealth of information currently available is widely disseminated. This book serves the purpose with very detailed information on and illustrations of 35 selected forage legume species that will contribute to more efficient and viable grassland farming.

Molecular Techniques in Food Biology: Safety, Biotechnology, Authenticity & Traceability explores all aspects of microbe-food interactions, especially as they pertain to food safety. Traditional morphological, physiological, and biochemical techniques for the detection, differentiation, and identification of microorganisms have severe limitations. As an alternative, many of those responsible for monitoring food safety are turning to molecular tools for identifying foodborne microorganisms. This book reviews the latest molecular techniques for detecting, identifying, and tracing microorganisms in food, addressing both good foodborne microbes, such as those used for fermentation and in probiotics, and harmful ones responsible for foodborne illness and food quality control problems. Molecular Techniques in Food Biology: Safety, Biotechnology, Authenticity & Traceability brings together contributions by leading international authorities in food biology from academe, industry, and government. Chapters cover food microbiology, food mycology, biochemistry, microbial ecology, food biotechnology and bio-processing, food authenticity, food origin traceability, and food science and technology. Throughout, special emphasis is placed on novel molecular techniques relevant to food biology research and for monitoring and assessing food safety and quality. Brings together contributions from scientists at the leading edge of the revolution in molecular food biology Explores how molecular techniques can satisfy the dire need to deepen our understanding of how microbial communities develop in foods of all types and in all forms Covers all aspects of food safety and hygiene, microbial ecology, food biotechnology and bio-processing, food authenticity, food origin traceability, and more Fills a yawning gap in the world literature on food traceability using molecular techniques This book is an important working resource for professionals in agricultural, food science, biomedicine, and government involved in food regulation and safety. It is also an excellent reference for advanced students in agriculture, food science and food technology, biochemistry, microbiology, and biotechnology, as well as academic researchers in those fields.

Molecular Associations in Biology provides information pertinent to molecular biology. This book discusses several topics, including DNA replication, calculation of intermolecular energies, and thermodynamic parameters of polynucleotides. Organized into 35 chapters, this book starts with an overview of the specific association of the purine and pyrimidine bases in the nucleic acids, which provides the basis for storage, expression, and transmission of genetic information. This text then explores the secondary structures, interactions, and replication processes of nucleic acids. Other chapters consider the complex biological process of protein synthesis. This book discusses as well the methods of photodynamic action, which is significant in detecting energy transfer from dye to the biomolecule and identifying the free radicals produced. The final chapter deals with the macroscopic properties of molecular systems, which refer to such concepts as volume, mass, pressure, temperature, and pH. This book is a valuable resource for biophysicists, organic chemists, biochemists, and biologists.