ABSTRACT: Technical alternatives for the production ofSingle-Cell Protein (SCP), and the operational and economicaspects of these alternatives are discussed. Topics include:1) SCP for human and animal consumption; 2) cellcollection, fermentation, and protein extraction techniques;3) production and processing methods; 4) uses of carob,agricultural wastes, cane, coffee, whey, sulfite liquor,methane, and yeast in SCP production; 5) evaluation methods;6) marketing procedures; and 7) international applicationsand guidelines.

In early 1973, I returned to Israel from a post-doctoral fellowship at Harvard University, and was accepted as a lecturer in the Department of Applied Microbiology at the Hebrew University of Jerusalem. Shortly after my return, Professor Richard Mateles, who at that time was head of the Department, suggested that I purchase a good and comprehensive book on single cell protein (SCP) in order to expand my general knowledge in the subject I had started then to work on; that was microbial utilization of one-carbon (C ) compounds. l Naturally, I took his advice (after all, he was the Boss) and bought the book, which was the only general book published on this subject at that time, and was based on papers presented at the First International Conference on Single Cell Protein, held at the Massachussetts Institute of Technology (M.I.T.), on October 1967 (Mateles and Tannenbaum, editors) [1]. Through this book I became acquainted with the world's hunger problem that existed in the past, and ways in which it was to be solved by SCP products prepared from CO , fossil-based raw 2 materials, and from wastes.

This book focuses on bioconversion of lignocellulosic residues into single-cell protein, which offers an alternative to conventional proteins (such as soybean meal, egg protein or meat protein in animal feed) that is not affected by the climate. It provides an overview of the general uses of lignocellulosic residues and their bioconversion into single-cell protein using microorganisms, as well as the recovery of the valuable by-products. It also explores the benefits and potential drawbacks of single-cell protein, with an emphasis on the economic advantages of such processes. Given its multidisciplinary scope, the book represents a valuable resource for academics and industry practitioners interested in the production of single-cell protein from lignocellulosic residues.

Cellular agriculture, also called lab-grown food, promises to provide alternative food options to current agriculture practices. Cellular agriculture is food grown in laboratories and bioreactors rather than on fields, relying on cultivation of cells under controlled conditions, with minimal use of natural resources and lower greenhouse gas emission costs than in traditional practices. It gives us the prospect of consuming the same foods such as a dairy ice cream or a burger. And it can further broaden the variety of textures, flavors, nutrition, and health-promoting aspects that food can deliver. Cellular Agriculture: Lab-Grown Foods gives an overview of the broad range of approaches to cellular agriculture, the current state of scale and regulations, and the results it brings about in terms of environmental footprint and consumer attitudes. Cellular Agriculture: Lab-Grown Foods was organized by Solar Foods, a food-tech company that develops a cell-based food protein produced from CO2 and electricity. A fruitful collaboration with VTT Technical Research Center of Finland Ltd allowed conceptualizing and streamlining of the written and visual content in the book.

This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. The book also includes chapters covering a suite of biochemical and biophysical methods capable of making an entire gamut of proteomic measurements, including analysis of protein abundance or expression, protein interaction networks, post-translational modifications, translocation and enzymatic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Single Cell Protein Analysis: Methods and Protocols is useful to researchers and students in biological and biomedical sciences who have an interest in proteomic measurements in cells.