Modern Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis was first introduced in 1954 the considerations were: 1. the dependence of scientific progress in biology on the improvement of ex isting and the introduction of new methods; 2. the difficulty in finding many new analytical methods in specialized jour nals which are normally not accessible to experimental plant biologists; 3. the fact that in the methods sections of papers the description of methods is frequently so compact, or even sometimes so incomplete that it is dif ficult to reproduce experiments. These considerations still stand today. The series was highly successful, seven volumes appearing between 1956 and 1964. Since there is still today a demand for the old series, the publisher has decided to resume publication of Modern Methods of Plant Analysis. It is hoped that the New Series will be just as acceptable to those working in plant sciences and related fields as the early volumes undoubtedly were. It is difficult to single out the major reasons for success of any publication, but we believe that the methods published in the first series were up-to-date at the time and presented in a way that made description, as applied to plant material, com plete in itself with little need to consult other publications. Contribution authors have attempted to follow these guidelines in this New Series of volumes.

This practical guide covers the commonly used detection methods for seed-transmitted viruses and viroids that affect both tropical and temperate crops. It contains 25 complete step-by-step procedures for biological, serological and molecular techniques to detect and identify such viruses. Combining helpful practical notes with more detailed explanations of the principles behind the techniques, the book describes the general characteristics of seed-transmitted viral diseases and discusses outlines for the organization and interpretation of seed health assays. The techniques reviewed are also applicable to non-seed-transmitted viral agents.

An essential reference for students, seed technologists, researchers, and seed industry personnel, this comprehensive guide outlines the most widely performed modern seed quality tests, explores the principles behind them, the history of seed testing, why seeds are tested and when, and sampling, sub-sampling, seed laboratory management, accreditation, and seed quality assurance programs. The authors describe statistical applications to seed testing and tolerances, and they provide a detailed morphological and structural description of seed formation and development. The book examines the testing of genetic traits and transgenic seeds, including DNA and protein genetic purity tests, and cultivar purity identification for conventional seeds. In addition to the most common seed purity and viability tests, tests for seed and seedling vigor, seed-borne diseases and seed moisture determination are also discussed.

This Joint ISTA and FAO Handbook provides comprehensive guidelines for the setting up and managing of seed testing laboratories, including those that cater to small- and medium-scale seed enterprises and farmers’ cooperatives, which operate in low input production systems. The adherence to the guidelines by seed testing laboratories results in the use of uniform procedures across the board thereby facilitating inter-laboratory transferability of data. This harmonization enhances confidence in seed quality assurance mechanisms. The publication is an update of a previous one that was also jointly published by both organizations in 1969 and revised in 1979 and 1983. Its utility is enhanced by a set of new up-to-date information that builds upon existing data. The Handbook provides guidance on the range of seed testing equipment, procedures, and management systems that have become available in the several decades since the previous editions. Seed testing is a critical component of the seed value chain and is essential for the production of quality seeds, i.e. seeds that are alive, can germinate and produce vigorous seedlings; are healthy and come from lots that meet set thresholds for genetic and analytical purity. The Handbook covers all aspects of seed testing, from laboratory design and equipment selection to sample preparation, testing methods, and data analysis. Importantly, the publication also addresses the challenges of testing seeds in low input systems and provides guidance on how to adapt procedures to these settings. Therefore, it serves as a reference material and training resource for everyone involved in seed quality assurance procedures, in particular the personnel of seed testing laboratories and regulatory agencies; seed producers and farmers. The updated publication represents a significant improvement over previous guidelines, as it incorporates advances in technology and provides guidance on a broader range of testing procedures. The guidelines are designed to be adaptable to different contexts and seed types and include recommendations for quality management systems, proficiency testing, and inter-laboratory comparisons, which are essential for ensuring the accuracy and reliability of seed testing results.

Rice seed health and quarantine; The rice plant and its environment; Equipment; Samples and sampling; dry seed inspection; Fungi; Bacteria; Nematodes; Viruses and mycoplasmalike organisms; Field inspection; Seed treatment; Weed seed; Insect pests; Fungal pathogens; Bacterial pathogens; Nematode pest; Organisms causing grain discoloration and damage.

This Fourth Edition of Principles of Seed Science and Technology, like the fIrst three editions, is written for the advanced undergraduate student or lay person who desires an introduction to the science and technology of seeds. The fIrst nine chapters present the seed as a biological system and cover its origin, development, composition, function (and sometimes nonfunction), performance and ultimate deterioration. The last nine chapters present the fundamentals of how seeds are produced, conditioned, evaluated and distributed in our modern agricultural society. Two new chapters have been added in this fourth edition, one on seed ecology and the second on seed drying. Finally, revisions have been made throughout to reflect changes that have occurred in the seed industry since publication of the Third Edition. Because of the fundamental importance of seeds to both agriculture and to all of society, we have taken great care to present the science and technology of seeds with the respect and feeling this study deserves. We hope that this feeling will be communicated to our readers. Furthermore, we have attempted to present information in a straight-forward, easy-ta-read manner that will be easily understood by students and lay persons alike. Special care has been taken to address both current state-of-the-art as well as future trends in seed technology.