RNA Interference from Biology to Therapeutics
Title | RNA Interference from Biology to Therapeutics PDF eBook |
Author | Kenneth A. Howard |
Publisher | Springer Science & Business Media |
Pages | 348 |
Release | 2012-10-19 |
Genre | Medical |
ISBN | 1461447437 |
The enormous potential of siRNA as a therapeutic has led to an explosion of interest from the scientific community. There has been intense interest from Big Pharma to capitalise on this new technology but the fact remains that delivery is a key determinant in realizing the full clinical potential of RNA interference. There is an urgent need for better delivery methods to take this technology forward. This book addresses the role of different RNAi molecules in cellular processes as rational for diagnostic and therapeutic approaches. This book will cover RNAi therapeutic design to optimize siRNA potency and reduce off-target effects and current delivery technologies to overcome both intracellular and extracellular barriers. The reader will gain an insight into RNA interference from the cellular mechanisms to screening to siRNA design right through to diagnostic and therapeutic applications.
RNAi Technology
Title | RNAi Technology PDF eBook |
Author | R. K. Gaur |
Publisher | CRC Press |
Pages | 380 |
Release | 2016-04-19 |
Genre | Science |
ISBN | 1439873259 |
RNAi technology is used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. Introducing new technology in the study of RNA
Therapeutic Applications of RNAi
Title | Therapeutic Applications of RNAi PDF eBook |
Author | John F. Reidhaar-Olson |
Publisher | Humana Press |
Pages | 0 |
Release | 2009-07-09 |
Genre | Medical |
ISBN | 9781603272940 |
The phenomenon of RNA interference has rapidly moved from groundbreaking scientific discovery to promising therapeutic approach. However, even as RNAi-based drugs enter the clinic, significant challenges remain, particulary in the area of delivery. Therapeutic Applications of RNAi provides detailed protocols in key areas of current focus, including testing of delivery vehicles, identification of appropriate model systems, and evaluation of the effects of RNAi in vivo. Produced by a team of internationally renowned authors, the volume describes the therapeutic applications of RNAi and potential pitfalls in oncology, viral infections and CNS disease, using a variety of delivery methods, including liposomes, peptide-based nanoparticles, polycationic polymers, and viral vehicles. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Therapeutic Applications of RNAi is an ideal guide for scientists attempting to solve the numerous challenges in this field and revolutionize the treatment of disease.
Advanced Delivery and Therapeutic Applications of RNAi
Title | Advanced Delivery and Therapeutic Applications of RNAi PDF eBook |
Author | Kun Cheng |
Publisher | John Wiley & Sons |
Pages | 660 |
Release | 2013-04-19 |
Genre | Science |
ISBN | 1118610733 |
Commonly used by researchers to develop technologies for modifying and studying genetic process, RNA interference (RNAi) has many potential uses in medicine, biotechnology, and functional genomics. This book covers all essential aspects involved in the development of RNAi therapeutics, providing detailed guidance on the challenges and opportunities of bringing RNAi technologies from bench to clinic. It explores the design and mechanism of RNAi molecules, delivery strategies, and therapeutic applications in various diseases. Preclinical, regulatory, market, and intellectual aspects of RNAi technologies are also covered.
RNA Interference (RNAi)
Title | RNA Interference (RNAi) PDF eBook |
Author | Wiley |
Publisher | John Wiley & Sons |
Pages | 214 |
Release | 2013-11-26 |
Genre | Science |
ISBN | 1118829441 |
The 2006 Nobel Prize in Physiology or Medicine was awarded to Andrew Z. Fire and Craig C. Mello for their discovery of RNA interference (RNAi). RNAi is a biological process in which RNA molecules can silence (inhibit) or up- or down-regulate gene expression, typically by causing the destruction of specific messenger RNA (mRNA) molecules. This collection of articles begins with an introduction to RNAi, and proceeds to describe uses of this technology in various approaches to disease treatment, including gene therapy. Several laboratory protocols for silencing genes via RNAi are also provided, as are protocols for down-regulating and then rescuing those down-regulated genes, which demonstrates specificity of the approach. This e-book — a curated collection from eLS, WIREs, and Current Protocols — offers a fantastic introduction to the field of RNA interference for students or interdisciplinary collaborators.
Cellular RNA Interference Mechanisms
Title | Cellular RNA Interference Mechanisms PDF eBook |
Author | Dirk Grimm |
Publisher | Academic Press |
Pages | 264 |
Release | 2011-08-26 |
Genre | Medical |
ISBN | 0124157955 |
This volume explores some of the most exciting recent advances in basic research on cellular RNA interference mechanisms and how this knowledge is leading to advances in the various fields. This series provides a forum for discussion of new discoveries, approaches, and ideas Contributions from leading scholars and industry experts Reference guide for researchers involved in molecular biology and related fields
RNA Interference and CRISPR Technologies
Title | RNA Interference and CRISPR Technologies PDF eBook |
Author | Mouldy Sioud |
Publisher | Humana |
Pages | 486 |
Release | 2021-02-15 |
Genre | Medical |
ISBN | 9781071602928 |
This volume explores the uses of RNAi and CRISPR interferences as a general method for inhibiting gene expression, with focus on their biological functions, design, chemical modifications, delivery, and preclinical/clinical applications. In addition to relevant backgrounds, the chapters in this book discuss simple and accurate protocols dealing with the latest advances in RNAi and CRISPR applications and look at how these methods differ from one another. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, RNA Interference and CRISPR Technologies: Technical Advances and New Therapeutic Opportunities is a valuable resource for any scientist, teachers, graduate student, postdoc, and clinician interested in this field. This book also benefits anyone in research and development in biotech and pharmaceutical companies who want to understand more about these technologies, and their applications in biology and medicine.