This detailed volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and express guide RNAs in a conditional manner. It concludes with methods for measuring off-target guide RNA activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and essential, CRISPR Guide RNA Design: Methods and Protocols provides a comprehensive pipeline for guide RNA design and aims to be an invaluable resource in applying this powerful technology to basic research and therapeutic applications.

This open access volume gathers a variety of models, delivery systems, and approaches that can be used to assess RNA technology for exploiting antisense as a therapeutic intervention. Beginning with a section on the design of antisense technology and their delivery, the book continues by covering model systems developed to evaluate efficacy, both in vivo and in vitro, as well as methods to evaluate preclinically the toxicity associated with these new potential drugs, and intellectual property considerations. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antisense RNA Design, Delivery, and Analysis provides basic knowledge and a large collection of methods to facilitate the work of newcomers to this vibrant and expanding field. This book was conceived thanks to the network DARTER (Delivery of Antisense RNA Therapeutics). DARTER is funded by the EU Cooperation of Science and Technology (COST), which aims to enhance interaction and collaborations between researchers in Europe and other countries.

Central to the synthesis of proteins, the performance of catalysis, and many other physiological processes, the aberrant expression of which can be linked to human diseases including cancers, RNA has proven to be key target for therapeutics as well as a tool for therapy. In RNA Therapeutics: Function, Design, and Delivery, expert contributors from a broad spectrum of scientific backgrounds highlight the roles that messenger RNAs and small RNAs can play in biology and medicine. While covering the five major RNA-based drugs, namely the use of ribozymes to cleave and/or correct mRNA transcript, the use of siRNA for targeted silencing of gene transcripts, the use of aptamers, like short RNA molecules, for neutralizing the protein functions, the use mRNA-transfected DCs to activate immune system against tumor cells, as well as the use of RNA to reprogram T and/or DC cell function, this extensive volume brings together the fields of coding (mRNA) and non-coding RNA such as ribozymes, RNAse P, siRNAs, and miRNAs into one convenient source. Written in the highly successful Methods in Molecular BiologyTM series format, the cutting-edge protocol chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and practical tips on troubleshooting and avoiding known pitfalls. Also, the book contains several excellent reviews for teaching purposes. Authoritative and comprehensive, RNA Therapeutics: Function, Design, and Delivery provides key models and tools which will assist researchers in increasing our understanding of RNA functions, modifications, and their involvement in diseases in order to lead to the design of vital new RNA-based therapeutics.

This book constitutes the refereed proceedings of the 5th International Workshop on Ant Colony Optimization and Swarm Intelligence, ANTS 2006, held in Brussels, Belgium, in September 2006. The 27 revised full papers, 23 revised short papers, and 12 extended abstracts presented were carefully reviewed and selected from 115 submissions.

Zusammenfassung: This book constitutes the proceedings of the 28th Annual International Conference on Research in Computational Molecular Biology, RECOMB 2024, held in Cambridge, MA, USA, during April 29-May 2, 2024. The 57 full papers included in this book were carefully reviewed and selected from 352 submissions. They were organized in topical sections as follows: theoretical and foundational algorithm contributions and more applied directions that engage with new technologies and intriguing biological questions

Designer Robin Wilson shares tips and tricks to detoxify and beautify your interior spaces and to help you create a more stylish and healthy home. Author Robin Wilson, plagued by allergies and asthma since infancy, has become an expert in Clean Design—creating allergen-reduced home environments that comfort and protect families. Using Wilson’s four principles of design—sustainable, reusable, recyclable, and nontoxic—every room in the home, from attic to basement and entryway to bedroom, can look sensational and stylish while reducing “your wheezing and sneezing.” Clean Design leads to amazing results: fewer allergy symptoms and asthma attacks, easier breathing, and better overall health. Whether building a new home, renovating, or simply updating the design of their current home, Wilson offers suggestions for the best eco-friendly, healthy, nontoxic furniture, wall and floor coverings, and window treatments to dramatically improve indoor air quality and reduce household exposure to dust, pet dander, pollen, mold, dust mites, smoke, household chemicals, airborne toxins, and other allergy and asthma triggers. By making eco-friendly design choices, you will not only protect yourself and your loved ones, but also contribute to the health and sustainability of our planet at the same time. Using traditional solutions, new methods, and some of her grandmother’s secrets, Wilson empowers you to incorporate Clean Design into your home, influencing your lifestyle, increasing your family’s wellness, and proving that “eco-friendly can be beautiful!”