RNA Biochemistry and Biotechnology describes various aspects of nucleic acid and protein structure, mainly RNA structure and proteins, interacting with specific RNA species. Papers deal with DNA protein interactions, telomerase, aminoacyl-tRNA synthetases, elongation factor Tu, DNA repair, RNA structure, NMR technology, RNA aptamer interaction of biological macromolecules with metal ions. Two papers deal with theoretical aspects of RNA structure production and computer modelling. Many papers describe the possibility of commercial application of RNA biotechnology. One article discusses the impact of direct democracy on basic science supporting biotechnology. Readership: Advanced graduate students, Ph.D. students and young scientists as well as specialists in the field.

Enzymes in RNA Science and Biotechnology, Part B, Volume 692 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as Quantitative base-resolution sequencing technology for mapping pseudouridines in mammalian mRNA, Quantitative base-resolution DAMM-seq for mapping RNA methylations in tRNA and mitochondrial dsRNA, Discovering RNA modification enzymes using a comparative genomics approach, Functional analysis of tRNA modification enzymes using mutational profiling, and Fluorescent labeling of tRNA for rapid kinetic interaction studies with tRNA-binding proteins. Other chapters cover Enzymatic Synthesis of RNA Standards for Mapping and Quantifying RNA Modifications in Sequencing Analysis, Characterizing RNase L-mediated mRNA decay in single cells, Characterization of RNase J, Pri-miRNA cleavage assays for the Microprocessor complex, The pre-miRNA cleavage assays for DICE, Methods for study of ribonuclease targeting chimeras, and Profiling the in vivo RNA interactome associated with the endoribonuclease RNase III in Staphylococcus aureus. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in Methods in Enzymology series Updated release includes the latest information on Enzymes in RNA Science and Biotechnology

The central role of RNA in many cellular processes, in biotechnology, and as pharmaceutical agents, has created an interest in experimental methods applied to RNA molecules. This book provides scientists with a comprehensive collection of thoroughly tested up-to-date manuals for investigating RNA-protein complexes in vitro. The protocols can be performed by researchers trained in standard molecular biological techniques and require a minimum of specialized equipment. The procedures include recommendation of suppliers of reagents.

In the past few decades there has been incredible growth in "bionano"-related research, which has been accompanied by numerous publications in this field. Although various compilations address topics related to deoxyribonucleic acid (DNA) and protein, there are few books that focus on determining the structure of ribonucleic acid (RNA) and using RN

Non-coding RNAs (ncRNAs) are RNA molecules which are not translated into proteins. They are classified into two types based on their length, including short non-coding RNAs comprising microRNAs (miRNAs) and long non-coding RNAs (lncRNAs). They play a role in gene regulation at various levels, ranging from epigenetic gene silencing to post-transcriptional modulation of mRNA stability. The ncRNAs contribute in the regulation of cell physiology and shape cellular functions. They play an important role in brain development and function, and are also associated with the pathogenesis of psychiatric disorders. Several ncRNAs including circRNAs, miRNAs and lncRNAs have been implicated in regulatory functions. Furthermore, they are believed to play a role in Alzheimer's disease and cancer. This book explores all the important aspects of the biochemistry of RNA. It presents researches and studies performed by experts across the globe. The extensive content of this book provides the readers with a thorough understanding of this topic.

RNA plays a central, and until recently, somewhat underestimated role in the genetics underlying all forms of life on earth. This versatile molecule not only plays a crucial part in the synthesis of proteins from a DNA template, but is also intrinsically involved in the regulation of gene expression, and in catalysis.This landmark text provides a systematic overview of the exciting and rapidly moving field of RNA biology. For the secondedition of Molecular Biology of RNA more introductory material has been incorporated at the beginning of the text, while new material has been included throughout - particularly in relation to RNA bindingdomains, non-coding RNAs, and the connection between RNA biology and epigenetics. Finally, a new closing chapter discusses how exciting new technologies are being used to explore current topical areas of research.