Transfer RNA in Protein Synthesis is a comprehensive volume focusing on important aspects of codon usage, selection, and discrimination in the genetic code. The many different functions of tRNA and the specialized roles of the corresponding codewords in protein synthesis from initiation through termination are thoroughly discussed. Variations that occur in the initiation process, in reading the genetic code, and in the selection of codons are discussed in detail. The book also examines the role of modified nucleosides in tRNA interactions, tRNA discrimination in aminoacylation, codon discrimination in translation, and selective use of termination codons. Other topics covered include the adaptation of the tRNA population to codon usage in cells and cellular organelles, the occurence of UGA as a codon for selenocysteine in the universal genetic code, new insights into translational context effects and in codon bias, and the molecular biology of tRNA in retroviruses. The contributions of outstanding molecular biologists engaged in tRNA research and prominent investigators from other scientific disciplines, specifically retroviral research, make Transfer RNA in Protein Synthesis an essential reference work for microbiologists, biochemists, molecular biologists, geneticists, and other researchers involved in protein synthesis research.

Transfer RNA in Protein Synthesis is a comprehensive volume focusing on important aspects of codon usage, selection, and discrimination in the genetic code. The many different functions of tRNA and the specialized roles of the corresponding codewords in protein synthesis from initiation through termination are thoroughly discussed. Variations that occur in the initiation process, in reading the genetic code, and in the selection of codons are discussed in detail. The book also examines the role of modified nucleosides in tRNA interactions, tRNA discrimination in aminoacylation, codon discrimination in translation, and selective use of termination codons. Other topics covered include the adaptation of the tRNA population to codon usage in cells and cellular organelles, the occurence of UGA as a codon for selenocysteine in the universal genetic code, new insights into translational context effects and in codon bias, and the molecular biology of tRNA in retroviruses. The contributions of outstanding molecular biologists engaged in tRNA research and prominent investigators from other scientific disciplines, specifically retroviral research, make Transfer RNA in Protein Synthesis an essential reference work for microbiologists, biochemists, molecular biologists, geneticists, and other researchers involved in protein synthesis research.

RNA and Protein Synthesis is a compendium of articles dealing with the assay, characterization, isolation, or purification of various organelles, enzymes, nucleic acids, translational factors, and other components or reactions involved in protein synthesis. One paper describes the preparatory scale methods for the reversed-phase chromatography systems for transfer ribonucleic acids. Another paper discusses the determination of adenosine- and aminoacyl adenosine-terminated sRNA chains by ion-exclusion chromatography. One paper notes that the problems involved in preparing acetylaminoacyl-tRNA are similar to those found in peptidyl-tRNA synthesis, in particular, to the lability of the ester bond between the amino acid and the tRNA. Another paper explains a new method that will attach fluorescent dyes to cytidine residues in tRNA; it also notes the possible use of N-hydroxysuccinimide esters of dansylglycine and N-methylanthranilic acid in the described method. One paper explains the use of membrane filtration in the determination of apparent association constants for ribosomal protein-RNS complex formation. This collection is valuable to bio-chemists, cellular biologists, micro-biologists, developmental biologists, and investigators working with enzymes.

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