Allelopathy is a new field of science, as the term Allelopathy was coined by Prof. Hans Molisch, a German Plant Physiologist in 1937. However, no standard methods are being used by various workers due to lack of compendium on the Techniques, hence, the results obtained are not easily comparable with each others. Till now lot of allelopathy resech has been done in various fields of Agricultural and Plant Sciences. However, there is no compilation of various Research Methods used. Every scientist is conducting research in his own way. It is causing lot of problems to researchers working in underdeveloped/Third World Countries in small towns without Library facilities. Therefore, to make available the standard methods for conducting allelopathy research independently, this multi-volume book has been planned. Since allelopathy is multi-disciplinary area of research, hence, volumes have been planned for each discipline. Prof. S.S. Narwal has planned this multi-volume Book Research Methods in Plant Sciences : Allelopathy. Three volumes (Volume 1. Soil Analysis, Volume 2. Plant Protection and Volume 3. Plant Pathogens) of this Book were released during the IV. International Allelopathy Conference, August 23-25, 2004 at Haryana Agricultural University, Hisar-125004, India. Volumes 4. Plant Analysis and Volume 5. Plant Physiology will be released in November, 2006. Three volumes (Volume 6. Cell Diagnostics, Volume 7. Chemistry Methods and Volume 8. Weed Studies) are under preparation. This volume of 28 Chapters, is divided into 7 Sections. Section I. Seed Physiology, includes 5 chapters describing the structure of seed, optimum conditions for seed germination, physiological and biochemical changes at cellular level. Section II. Growth and Development, describes leaf area, growth indices, senescence and abscission. Allelochemicals, present in soil or plant, can create chemical stress which may change the plant water status, plasma membrane properties, chlorophyll stability and waxes present on the organ surface. Methods to determine all these parameters are described in next 4 chapters in Section III. Stress Physiology. These sites can be explored by estimating chlorophyll content, chlorophyll fluorescence, photosystems I and II activity, carbon dioxide exchange rate, activity of CO2 fixing enzymes, intermediate metabolite level, photosynthate partitioning, respiration and finally the crop growth dynamics. Methods to determine extent of all these sites are explained in 7 chapters in Section IV. Gas Exchange Processes. The main cause of changed physiological process is at the gene level, for which estimation of nucleic acids is very critical. It is briefly explained in section V. Biochemical Estimation. Section VI. Microtomy and Histochemistry, has 7 chapters. Basic procedure to process the test plant material for microtomy, use of light and electron microscopy to study cellular changes, measurement of cellular dimensions, stomatal index and frequency, pollen viability and in vivo pollen germination and histochemical localization of important enzymes and metabolites are the core topics. Currently, tissue cultures are commonly used to study the precise effect of allelochemicals on callus growth and differentiation. To achieve these objectives techniques of tissue cultures is described under section VI. Tissue Culture.

Allelopathy is a new field of science, as the term Allelopathycoined by Prof. Hans Molisch, a German Plant Physiologist in 1937. However, no standard methods are being used by various workers due to lack of compendium on the Techniques, hence, the results obtained are not easily comparable with each others. Till now lot of allelopathy resech has been done in various fields of Agricultural and Plant Sciences. However, there is no compilation of various Research Methods used. Every scientist is conducting research in his own way. It is causing lot of problems to researchers working in underdeveloped/Third World Countries in small towns without Library facilities. Therefore, to make available the standard methods for conducting allelopathy research independently, this multi-volume book has been planned. Since allelopathy is multi-disciplinary area of research, hence, volumes have been planned for each discipline. Prof. S.S. Narwal has planned this multi-volume Book Research Methods in Plant Sciences : Allelopathy. Three volumes (Volume 1. Soil Analysis, Volume 2. Plant Protection and Volume 3. Plant Pathogens) of this Book were released during the IV. International Allelopathy Conference, August 23-25, 2004 at Haryana Agricultural University, Hisar-125004, India. Volumes 4. Plant Analysis and Volume 5. Plant Physiology will be released in November, 2006. Three volumes (Volume 6. Cell Diagnostics, Volume 7. Chemistry Methods and Volume 8. Weed Studies) are under preparation. This book consists of 12 Chapters, describing the methods to analyse various nutrients in plants. The Book is devided into two Sections : General and Determination of Plant nutrients. The Section I. General, provides very elementary and basic information about the various equipments and apparatus used to determine plant nutrients and preparation of Reagents etc. Further, methods of collecting plant samples and their digestion have been described. In Section II. Determination of Plant Nutrients, 8 Chapters describes methods of determining various plant nutrients (Carbon, Nitrogen, Phospherus, Potassium, Sodium, Calcium, Magnesium, Sulphur, Micronutrients and Toxic metals).

Physiological plant ecology is primarily concerned with the function and performance of plants in their environment. Within this broad focus, attempts are made on one hand to understand the underlying physiological, biochemical and molecular attributes of plants with respect to performance under the constraints imposed by the environment. On the other hand physiological ecology is also concerned with a more synthetic view which attempts to under stand the distribution and success of plants measured in terms of the factors that promote long-term survival and reproduction in the environment. These concerns are not mutually exclusive but rather represent a continuum of research approaches. Osmond et al. (1980) have elegantly pointed this out in a space-time scale showing that the concerns of physiological ecology range from biochemical and organelle-scale events with time constants of a second or minutes to succession and evolutionary-scale events involving communities and ecosystems and thousands, if not millions, of years. The focus of physiological ecology is typically at the single leaf or root system level extending up to the whole plant. The time scale is on the order of minutes to a year. The activities of individual physiological ecologists extend in one direction or the other, but few if any are directly concerned with the whole space-time scale. In their work, however, they must be cognizant both of the underlying mechanisms as well as the consequences to ecological and evolutionary processes.

This book consists of 12 Chapters, describing the methods to analyse various nutrients in plants. The Book is divided into two Sections : General and Determination of Plant nutrients. The Section I. General, provides very elementary and basic information about the various equipments and apparatus used to determine plant nutrients and preparation of Reagents etc. Further, methods of collecting plant samples and their digestion have been described. In Section II. Determination of Plant Nutrients, 8 Chapters describes methods of determining various plant nutrients (Carbon, Nitrogen, Phosphorus, Potassium, Sodium, Calcium, Magnesium, Sulphur, Micronutrients and Toxic metals). It will prove very useful to under-graduate and post graduate students and teaching Faculty for Class Room and Laboratory experiments as well as for research.

Allelopathy is a new field of science, as the term 'Allelopathy' was coined by Prof. Hans Molisch, a German Plant Physiologist in 1937. Till now lot of Allelopathy research work has been done in various fields of Agricultural and Plant Sciences. However, there is no compilation of various Research Methods used. Every scientist is conducting research in his own way. It is causing lot of problems to researchers working in underdeveloped/Third World Countries in small towns without Library facilities. Therefore, to make available the standard methods for conducting allelopathy research independently, this multi-volume book has been planned. Since allelopathy is multi-disciplinary area of research, hence, volumes have been planned for each discipline. Prof. S.S. Narwal has planned this multi-volume Book Research Methods in Plant Sciences: Allelopahty. Three volumes (Volume 1. Soil Analysis, Volume 2. Plant Protection and Volume 3. Plant Pathogens) of this Book have been released during the IV. International Allelopathy Conference, 2004 at Hisar (India). Five volumes (Volume 4. Plant Analysis, Volume 5. Physiological Processes, Volume 6. Biochemical Processes, Volume 7. Forestry/Agroforestry Research and Volume 8. Isolation, Identification and Characterization of allelochemicals are under preparation. This volume has 11 Chapters, divided in three Sections viz., Entomology, Nematology and Weeds. It provides complete information about the various techniques used for Allelopathy Research in the field of Entomology, Nematology and Weeds. It is written in a simple and lucid language. It will be very useful to undergraduate and Post graduate students and Faculty for used in Class room and Laboratory experiments and research. We are thankful to Prof. G. S. Dhaliwal, Department of Entomology, Punjab Agricultural University, Ludhiana and Prof. V. Mojumder, Division of Nematology, Indian Agricultural Research Institute, New Delhi for Peer Review of Entomology and Nematology Manuscripts.

This volume has 11 Chapters, divided in three Sections viz., Entomology, Nematology and Weeds. It provides complete information about the various techniques used for Allelopathy Research in the field of Entomology, Nematology and Weeds. It is written in a simple and lucid language. It will be very useful to undergraduate and Post graduate students and Faculty for used in Class room and Laboratory experiments and research. We are thankful to Prof. G. S. Dhaliwal, Department of Entomology, Punjab Agricultural University, Ludhiana and Prof. V. Mojumder, Division of Nematology, Indian Agricultural Research Institute, New Delhi for Peer Review of Entomology and Nematology Manuscripts.

Allelopathy is a new field of science, as the term 'Allelopathy' was coined by Prof. Hans Molisch, a German Plant Physiologist in 1937. Till now lot of Allelopathy research work has been done in various fields of Agricultural and Plant Sciences. However, there is no compilation of various Research Methods used. Every scientist is conducting research in his own way. It is causing lot of problems to researchers working in underdeveloped/Third World Countries in small towns without Library facilities. Therefore, to make available the standard methods for conducting allelopathy research independently, this multi-volume book has been planned. Since allelopathy is multi-disciplinary area of research, hence, volumes have been planned for each discipline. Prof. S.S. Narwal has planned this multi-volume Book Research Methods in Plant Sciences: Allelopahty. Three volumes (Volume 1. Soil Analysis, Volume 2. Plant Protection and Volume 3. Plant Pathogens) of this Book have been released during the IV. International Allelopathy Conference, 2004 at Hisar(India). Five volumes (Volume 4. Plant Analysis, Volume 5. Physiological Processes, Volume 6. Biochemical Processes, Volume 7. Forestry/Agroforestry Research and Volume 8. Isolation, Identification and Characterization of allelochemicals are under preparation. Volume 1. Soil Analysis is consists of 20 Chapters, describing the methods to analyse various types of soil properties. The Book is devided into three Sections: General, Physio-chemical properties and Soil microbiology. It provides complete information for Soil Analysis in simple and lucid language. The Figures/ illustrations have been given at appropriate places in text. It will prove very useful to undergraduate and post graduate students and teaching Faculty for Class Room and Laboratory experiments as well as for research.