This title is a much needed update of Barbosa's self-published Manual of Basic Techniques in Insect Histology. It is a laboratory manual of 'traditional' and 'modern' insect histology techniques, completely revised using cutting-edge methodology carried out today and includes new immunohistochemical techniques not previously looked at. Insect Histology is designed as a resource for student and professional researchers, in academia and industry, who require basic information on the procedures that are essential for the histological display of the tissues of insects and related organisms.

Cell biology is moving at breakneck speed, and many of the results from studies on insects have helped in understanding some of the central problems of biology. The time is therefore ripe to provide the scientific community with a series of up-to-date, well illustrated reviews of selected aspects of the sub microscopic cytology of insects. The topics we have included fall into four general groups: seven chapters deal with gametogenesis, four concern develop ing somatic cells, seventeen chapters describe specialized tissues and organs, and three chapters cover cells in pathological states. These accounts are illustrated with over 600 electron micrographs. The more than 1100 pages in the two volumes of Insect Ultrastructure combined labors of 49 dedicated contributors from II countries. represent the These authors have digested and critically summarized a very large body of information, and some measure of this effort can be gained from consulting the bibliographies that close each of the 31 chapters. These contain 2400 publica tions authored by 1500 different scientists. However, before we congratulate ourselves on the advanced state of our knowledge, it is worth remembering that representatives of less than 0.01 % of the known species of insects have been examined with the electron microscope.

Manual of Techniques in Invertebrate Pathology, Second Edition, describes a wide range of techniques used in the identification, isolation, propagation/cultivation, bioassay, quantification, preservation, and storage of the major groups of entomopathogens, including entomophthorales, entomopathogenic fungi, entomopathogenic bacteria of the Bacilli, Nematode parasites, and pathogens and parasites of terrestrial molluscs. The book presents the perspectives of an international group of experts in the fields of invertebrate pathology, including microbiology, mycology, virology, nematology, biological control, and integrated pest management. Organized into 15 chapters, the book covers methods for the study of virtually every major group of entomopathogen, as well as methods for discovery and diagnosis of entomopathogens and the use of complementary methods for microscopy. It discusses the use of molecular techniques for identifying and determining phylogeny, factors that contribute to resistance to entomopathogens, and several other aspects of the science of invertebrate pathology. It also explains initial handling and diagnosis of diseased invertebrates, basic techniques in insect virology, and bioassay of bacterial entomopathogens against insect larvae. In addition, the reader is introduced to the use of bacteria against soil-inhabiting insects and preservation of entomopathogenic fungal cultures. The remaining chapters focus on research methods for entomopathogenic microsporidia and other protists, how the pathogenicity and infectivity of entomopathogens to mammals are tested, and preparations of entomopathogens and diseased specimens for more detailed study using microscopy. Experienced insect pathologists, biologists, entomologists, students, biotechnology personnel, technicians, those working in the biopesticide industry, and government regulators will find this manual extremely helpful. - Step-by-step instructions for the latest techniques on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens - New edition fully updated to address changes in the taxonomy of the vast majority of taxa - Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques - Provides extensive supplemental literature and recipes for media, fixatives and stains

This critical monograph presents a review and synthesis of the literature on the chemical, physical, and biological aspects of the integument of arthropod animals. The volume covers and collates material published through 1949 on the chemical and physical.