Protein Stability and Folding
Title | Protein Stability and Folding PDF eBook |
Author | Bret A. Shirley |
Publisher | |
Pages | 377 |
Release | 1995 |
Genre | Science |
ISBN | 9781592595273 |
In Protein Stability and Folding: Theory and Practice, world-class scientists present in a single volume a comprehensive selection of hands-on recipes for all of the major techniques needed to understand the conformational stability of proteins, as well as their three-dimensional folding. The distinguished contributors provide clear, step-by-step instructions along with many troubleshooting tips, alternative procedures, and informative explanations about why certain steps are necessary. Even highly skilled researchers will find many time-saving methods. Among the techniques discussed are fluorescent, ultraviolet, and infrared spectroscopy; HPLC peptide mapping; differential scanning calorimetry; and hydrogen exchange. Shirley's Protein Stability and Folding: Theory and Practice will ensure a significant difference in the outcome of your experiments, producing the result desired even for beginners.
Protein Structure, Stability, and Folding
Title | Protein Structure, Stability, and Folding PDF eBook |
Author | Kenneth P. Murphy |
Publisher | Springer Science & Business Media |
Pages | 258 |
Release | 2008-02-04 |
Genre | Science |
ISBN | 1592591930 |
In Protein Structure, Stability, and Folding, Kenneth P. Murphy and a panel of internationally recognized investigators describe some of the newest experimental and theoretical methods for investigating these critical events and processes. Among the techniques discussed are the many methods for calculating many of protein stability and dynamics from knowledge of the structure, and for performing molecular dynamics simulations of protein unfolding. New experimental approaches presented include the use of co-solvents, novel applications of hydrogen exchange techniques, temperature-jump methods for looking at folding events, and new strategies for mutagenesis experiments. Unique in its powerful combination of theory and practice, Protein Structure, Stability, and Folding offers protein and biophysical chemists the means to gain a more comprehensive understanding of some of this complex area by detailing many of the major techniques in use today.
Protein Structure, Stability, and Interactions
Title | Protein Structure, Stability, and Interactions PDF eBook |
Author | John W. Shriver |
Publisher | Humana |
Pages | 0 |
Release | 2010-11-19 |
Genre | Science |
ISBN | 9781617378553 |
In the areas of biochemistry and cell biology, characterizations of stability and molecular interactions call for a quantitative approach with a level of precision that matches the fine tuning of these interactions in a living cell. Supporting and up-dating previous Methods in Molecular BiologyTM volumes, Protein Structure, Stability, and Interactions approaches its subject with a focus on theory and practical applications for both established methods as well as exciting new procedures. The volume presents an overview of many techniques currently used to study protein stability and interactions, including scanning and titration calorimetry, spectroscopic methods, high field NMR, and analytical ultracentrifugation. As a volume of the highly successful Methods in Molecular BiologyTM series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Cutting-edge and easy to reference, Protein Structure, Stability, and Interactions is an ideal guide for all scientists interested in biomolecular interactions.
Rational Design of Stable Protein Formulations
Title | Rational Design of Stable Protein Formulations PDF eBook |
Author | John F. Carpenter |
Publisher | Springer Science & Business Media |
Pages | 218 |
Release | 2012-12-06 |
Genre | Medical |
ISBN | 1461505577 |
Recombinant proteins and polypeptides continue to be the most important class of biotechnology-derived agents in today's pharmaceutical industry. Over the past few years, our fundamental understanding of how proteins degrade and how stabilizing agents work has made it possible to approach formulation of protein pharmaceuticals from a much more rational point of view. This book describes the current level of understanding of protein instability and the strategies for stabilizing proteins under a variety of stressful conditions.
Formulation, Characterization, and Stability of Protein Drugs
Title | Formulation, Characterization, and Stability of Protein Drugs PDF eBook |
Author | Rodney Pearlman |
Publisher | Springer Science & Business Media |
Pages | 455 |
Release | 2006-04-11 |
Genre | Medical |
ISBN | 0306474522 |
Leading scientists offer detailed profiles of ten protein drugs currently in development. The case histories of these important new compounds are described from the perspective of their formulation, characterization, and stability. This ready reference also features recent data and an abundance of previously unpublished information. The in-depth coverage includes a highly useful compendium of degradation sites occurring in over 70 proteins. An invaluable aid in the rapid identification of potential `hot spots' in proteins, this accessible compilation allows for inspection of the protein's primary structure and preparation of a hydroflex plot.
Protein Stability
Title | Protein Stability PDF eBook |
Author | David S. Eisenberg |
Publisher | |
Pages | 334 |
Release | 1995 |
Genre | Science |
ISBN | 9780120342464 |
The topics covered by this volume include: protein destabilization at low temperatures; engineering the stability and function of Gene V Protein; free energy balance in protein folding; modelling protein stability as a heteropolymer collapse; stability of alpha helices; protein stability with T4 Lysozyme.
Molecular Biology of the Cell
Title | Molecular Biology of the Cell PDF eBook |
Author | |
Publisher | |
Pages | 0 |
Release | 2002 |
Genre | Cells |
ISBN | 9780815332183 |