The Protein Folding Problem and Tertiary Structure Prediction
Title | The Protein Folding Problem and Tertiary Structure Prediction PDF eBook |
Author | Kenneth M.Jr. Merz |
Publisher | Springer Science & Business Media |
Pages | 585 |
Release | 2012-12-06 |
Genre | Science |
ISBN | 1468468316 |
A solution to the protein folding problem has eluded researchers for more than 30 years. The stakes are high. Such a solution will make 40,000 more tertiary structures available for immediate study by translating the DNA sequence information in the sequence databases into three-dimensional protein structures. This translation will be indispensable for the analy sis of results from the Human Genome Project, de novo protein design, and many other areas of biotechnological research. Finally, an in-depth study of the rules of protein folding should provide vital clues to the protein fold ing process. The search for these rules is therefore an important objective for theoretical molecular biology. Both experimental and theoretical ap proaches have been used in the search for a solution, with many promising results but no general solution. In recent years, there has been an exponen tial increase in the power of computers. This has triggered an incredible outburst of theoretical approaches to solving the protein folding problem ranging from molecular dynamics-based studies of proteins in solution to the actual prediction of protein structures from first principles. This volume attempts to present a concise overview of these advances. Adrian Roitberg and Ron Elber describe the locally enhanced sam pling/simulated annealing conformational search algorithm (Chapter 1), which is potentially useful for the rapid conformational search of larger molecular systems.
Protein Folding
Title | Protein Folding PDF eBook |
Author | Grace E. Orellana |
Publisher | American Chemical Society |
Pages | 170 |
Release | 2024-05-08 |
Genre | Science |
ISBN | 0841296383 |
Life as we know it would not exist if proteins did not fold into functional three-dimensional structures, where α-helices, loops, and β-sheets act together to form active sites that drive a myriad of biochemical reactions in the cell. The failure of this process is linked to the pathology of various diseases, such as neurodegenerative disorders like Alzheimer’s, genetic conditions (like cystic fibrosis), and cancer. It is no wonder that close to $2 billion in worldwide research funding has been devoted over the last five years (2019–2025) to helping scientists understand the molecular details of protein folding, how it can fail in ways that promote disease in humans, and clinical paths to treat or prevent diseases linked to protein misfolding. This primer is prerequisite reading to the literature on this important topic for readers new to the field. Chapter one provides exposure to the three-dimensional structure of proteins; readers will learn how to identify secondary structures, protein motifs, and domains involved in biological function. Chapter two introduces methodologies to determine the three-dimensional structure of proteins; readers will learn modern techniques to determine the secondary structure composition and the orientation of atoms in three-dimensional space. By providing exposure to how the physical environment (i.e., chemical denaturants, pH, pressure, and temperature) controls protein denaturation, readers will learn how such information can be used to study the biophysical characteristics of proteins through various probes and methodologies.
Molecular Biology of The Cell
Title | Molecular Biology of The Cell PDF eBook |
Author | Bruce Alberts |
Publisher | |
Pages | 0 |
Release | 2002 |
Genre | Cytology |
ISBN | 9780815332183 |
Protein Folds
Title | Protein Folds PDF eBook |
Author | Henrik Bohr |
Publisher | CRC Press |
Pages | 352 |
Release | 1995-10-20 |
Genre | Medical |
ISBN | 9780849340093 |
Written by outstanding scientists in physics and molecular biology, this book addresses the most recent advances in the analysis of the protein folding processes and protein structure determination. Emphasis is also placed on modelling and presentation of experimental results of structural membrane bound proteins. Many color plates help to illustrate structural aspects covered including: Defining folds of protein domains Structure determination from sequence Distance geometry Lattice theories Membrane proteins Protein-Ligand interaction Topological considerations Docking onto receptors All analysis is presented with proven theory and experimentation. Protein Folds: A Distance-Based Approach is an excellent text/reference for biotechnologists and biochemists as well as graduate students studying in the research sciences.
Protein Folding
Title | Protein Folding PDF eBook |
Author | Charis Ghelis |
Publisher | Academic Press |
Pages | 580 |
Release | 2012-12-02 |
Genre | Science |
ISBN | 0323140920 |
Protein Folding aims to collect the most important information in the field of protein folding and probes the main principles that govern formation of the three-dimensional structure of a protein from a nascent polypeptide chain, as well as how the functional properties appear. This text is organized into three sections and consists of 15 chapters. After an introductory chapter where the main problems of protein folding are considered at the cellular level in the context of protein biosynthesis, the discussion turns to the conformation of native globular proteins. Definitions and rules of nomenclature are given, including the structural organization of globular proteins deduced from X-ray crystallographic data. Folding mechanisms are tentatively deduced from the observation of invariants in the architecture of folded proteins. The next chapters focus on the energetics of protein conformation and structure, indicating the principles of thermodynamic stability of the native structure, along with theoretical computation studies of protein folding, structure prediction, and folding simulation. The reader is also introduced to various experimental approaches; the reversibility of the unfolding-folding process; equilibrium and kinetic studies; and detection and characterization of intermediates in protein folding. This text concludes with a chapter dealing with problems specific to oligomeric proteins. This book is intended for research scientists, specialists, biochemists, and students of biochemistry and biology.
Protein Stability and Folding
Title | Protein Stability and Folding PDF eBook |
Author | Bret A. Shirley |
Publisher | |
Pages | 377 |
Release | 1995 |
Genre | Science |
ISBN | 9781592595273 |
In Protein Stability and Folding: Theory and Practice, world-class scientists present in a single volume a comprehensive selection of hands-on recipes for all of the major techniques needed to understand the conformational stability of proteins, as well as their three-dimensional folding. The distinguished contributors provide clear, step-by-step instructions along with many troubleshooting tips, alternative procedures, and informative explanations about why certain steps are necessary. Even highly skilled researchers will find many time-saving methods. Among the techniques discussed are fluorescent, ultraviolet, and infrared spectroscopy; HPLC peptide mapping; differential scanning calorimetry; and hydrogen exchange. Shirley's Protein Stability and Folding: Theory and Practice will ensure a significant difference in the outcome of your experiments, producing the result desired even for beginners.
Protein Folding and Metal Ions
Title | Protein Folding and Metal Ions PDF eBook |
Author | Cláudio M. Gomes |
Publisher | CRC Press |
Pages | 302 |
Release | 2016-04-19 |
Genre | Medical |
ISBN | 1439809658 |
The role of metal ions in protein folding and structure is a critical topic to a range of scientists in numerous fields, particularly those working in structural biology and bioinorganic chemistry, those studying protein folding and disease, and those involved in the molecular and cellular aspects of metals in biological systems. Protein Folding an