Ultrafiltration (UF) is a pressure-driven separation process in which membranes are used for a broad variety of applications, ranging from the processing of biological macromolecules to wastewater treatment. It has significant advantages over competing separation technologies. Food and biotechnological applications of UF account for nearly 40 percent of the current total usage. In the case of high value therapeutic protein and DNA products, the separation and purification costs can be as high as 80 percent of the total cost of production. Therefore, it makes economic sense to develop cost-effective and scaleable purification processes for such products. UF is used for protein concentration, protein desalting and protein fractionation (such as protein-protein separation). Concentration and desalting processes are technologically less demanding and have been widely used in the bioprocess industry for quite some time. Protein fractionation, on the other hand, is a challenging proposition and is definitely a more recent development. This text focuses primarily on protein fractionation.

Ultrafiltration is a pressure-driven, membrane-based separation process, which is used for a broad variety of applications, ranging from the processing of biological macromolecules to wastewater treatment. It has significant advantages over competing separation technologies. Food and biotechnological applications account for nearly 40% of the current total usage of ultrafiltration membranes. Protein bioseparation is an important component of this application segment. Ultrafiltration is used for protein concentration, desalting, clarification and fractionation (i.e. protein-protein separation). Concentration, desalting and clarification are technologically less demanding and have been in used in the bioprocess industry for some time. Protein fractionation, on the other hand, is a challenging proposition and is definitely a more recent development. This book focuses primarily on protein fractionation./a

Protein bioseparation is an important yet expensive activity especially in biotechnology industry. The production of protein is growth along with rapid commercialization and demand. Unfortunately, the industry having problem in term of cost and the production as the cost is extremely high but the production rate is low. The most effective way to solve this problem is by using membrane as the medium of separation. In this study, the effects for different mode of operations of ultrafiltration membranes - batch, single stage and two stages forward cascade. Amersham Quick Lab cross flow system was used to separate lysozyme by using 30 kDa polyethersulfone ultrafiltration membranes. For this research, the lysozyme is obtained from the cheapest source which is chicken egg white solution. Lysozyme is a very important protein in pharmaceutical field in order to produce a vaccine to prevent bacteria that inhabit the intestinal tract. In order to determine the most efficient configurations, the constant parameters are set for all configurations. The value of pH must be 10, the transmembrane pressure (TMP) is 1.0 bar, and the volume feed is 960 ml with the ratio of chicken egg white to sodium chloride 0.2 M is 1:31. The effective configuration can be determined by comparing the permeate flux, concentration of lysozyme in both permeate and retentate solution at 5 minutes after the separation process occurred and measurement of water flux. The analysis procedure is done using the UV-VIS spectrometer at wave length equal to 595 nm. The most effective configuration is two stages forward cascade which has the highest average permeate flux of 74.4 L/m2.h. Moreover, it also has percentage for concentration of lysozyme in permeate solution equal to 84% which is the biggest percentage of all configurations and the lowest value of water flux measurement after separation process compare to the benchmark value at TMP equal to 1.0 bar. The second configuration then is single stage and followed by batch at third place.

The Handbook of Membrane Separations: Chemical, Pharmaceutical, and Biotechnological Applications provides detailed information on membrane separation technologies as they have evolved over the past decades. To provide a basic understanding of membrane technology, this book documents the developments dealing with these technologies. It explores chemical, pharmaceutical, food processing and biotechnological applications of membrane processes ranging from selective separation to solvent and material recovery. This text also presents in-depth knowledge of membrane separation mechanisms, transport models, membrane permeability computations, membrane types and modules, as well as membrane reactors.

Chapter 1: Principles on membrane and membrane processes -- Chapter 2: Ultrafiltration -- Chapter 3: Microfiltration -- Chapter 4: Virus Filtration -- Chapter 5: Membrane chromatography -- Chapter 6: Membranes for the Preparation of Emulsions and Particles -- Chapter 7: Other Membrane Processes -- Chapter 8: Some Perspectives.

Bioseparations engineering deals with the scientific and engineering principles involved in large-scale separation and purification of biological products. It is a key component of most chemical engineering/biotechnology/bioprocess engineering programmes. This book discusses the underlying principles of bioseparations engineering written from the perspective of an undergraduate course. It covers membrane based bioseparations in much more detail than some of the other books on bioseparations engineering. Based largely on the lecture notes the author developed to teach the course, this book is especially suitable for use as an undergraduate level textbook, as most other textbooks are targeted at graduate students.

Promoting a continued and much-needed renaissance in biopharmaceutical manufacturing, this book covers the different strategies and assembles top-tier technology experts to address the challenges of antibody purification. • Updates existing topics and adds new ones that include purification of antibodies produced in novel production systems, novel separation technologies, novel antibody formats and alternative scaffolds, and strategies for ton-scale manufacturing • Presents new and updated discussions of different purification technologies, focusing on how they can address the capacity crunch in antibody purification • Emphasizes antibodies and innovative chromatography methods for processing