Longleaf pine (Pinus palustris Mill.) seeds are sensitive to damage during collection, processing, treatment, and storage. High-quality seeds are essential for successfully producing nursery crops that meet management goals and perform well in the field. Uniformity in the production of pine seedlings primarily depends on prompt and uniform seed germination, early seedling establishment, and a variety of cultural practices that are applied as the seedlings develop. The best collecting, handling, and processing methods maximize performance attributes and reduce the need for extensive nursery cultural practices to compensate for poor seed quality. Guidelines are presented that will help seed dealers, orchard managers, and nursery personnel produce high-quality longleaf pine seeds and improve the efficiency of nursery production.