Over the last few years several new instrumental techniques have been introduced for chromatographie separations. In addition, rapid developments in existing methods, such as preparative HPLC, have taken place. Despite these advances, how ever, a handbook covering the various preparative aspects ofthe new separation tech niques does not exist. This book is an attempt to fill the gap and to present a compila tion of modern separation techniques that will be useful for researchers faced with day-to-day preparative problems. Numerous examples of separations have been selected in order to show the possibilities (and also the limits) of each technique treated. These are often either applications from our own laboratory or else they reflect the approach we have been following for the isolation of natural products from plant sourees. Owing to the large number of published papers and the diversity of secondary plant constituents. an exhaustive survey of the literature has not been undertaken. We hope, however, that the examples selected will suggest to the reader which technique(s) and which conditions to choose for a particular isolation problem in the field of natural products. For invaluable help in the preparation of the manuscript for this book, we would like to thank Corinne Appolonia and Christine Marston. Lausanne, November 1985 K. Hostettmann M. Hostettmann A . . Marston Foreword Although not many people realize this, isolation and purification of biologically active materials is becoming increasingly crucial.

Density Gradient Centrifugation

This book provides a comprehensive and up-to-date review of recent trends of green science and technology. Worldwide deterioration of environment and global warming threaten our lifestyle and the survival of all creatures. In order to weather these problems, we need to construct a multidisciplinary approach involving the fusion of various advanced researches. The book begins with an overview on fundamental research about generation and utilization of renewable energy, protection of the earth's ecosystem for better coexistence with nature, development of artificial intelligence-based agriculture and molecular recognitionbased welfare and covers a wide range of innovative research on green science and technology.

Alternative treatment modes for antibiotic-resistant bacterial pathogens have become a public health priority. Bacteriophages are bacterial viruses that infect and lyse bacterial cells. Since bacteriophages are frequently bacterial host species-specific and can often also infect antibiotic-resistant bacterial cells, they could represent ideal antimicrobials for fighting the antibiotic resistance crisis. The medical use of bacteriophages has become known as phage therapy. It is widely used in Russia, where phage cocktails are sold in pharmacies as an over-the-counter drug. However, no phage product has been registered for medical purposes outside of the former Soviet Union. The current Special Issue of Viruses contains a collection of papers from opinion leaders in the field who explore hurdles to the introduction of phage therapy in western countries. The articles cover diverse topics ranging from patent to regulatory issues, the targeting of suitable bacterial infections, and the selection and characterization of safe and efficient phage cocktails. Phage resistance is discussed, and gaps in our knowledge of phage–bacterium interactions in the mammalian body are revealed, while other articles explore the use of phages in food production and processing.

The ability of polypeptides to form alternatively folded, polymeric structures such as amyloids and related aggregates is being increasingly recognized as a major new frontier in protein research. This new volume of Methods in Enzymology along with Part B (volume 412) on Amyloid, Prions and other Protein Aggregates continue in the tradition of the first volume (309) in containing detailed protocols and methodological insights, provided by leaders in the field, into the latest methods for investigating the structures, mechanisms of formation, and biological activities of this important class of protein assemblies. - Presents detailed protocols - Includes troubleshooting tips - Provides coverage on structural biology, computational methods, and biology

Each issue is packed with extensive news about important cancer related science, policy, politics and people. Plus, there are editorials and reviews by experts in the field, book reviews, and commentary on timely topics.