Fully revised, new edition presenting latest developments in medical biochemistry. Includes many new chapters and case reports. Previous edition published in 2006.

1. General General Instructions Laboratory Equipments and Procedures Buffers and pH Quality Control 2. Analysis of Urine and Cerebrospinal Fluid Normal Urine: Characteristics and Analysis Abnormal Constituents of Urine Screening of Urine for Inborn Errors of Metabolism and the Use of Paper Chromatography Composition of Cerebrospinal Fluid 3. Quantitative Experiments Principles of Colorimetry Principles of Spectrophotometer Estimation of Glucose Glucose Tolerance Test Estimation of Serum and Urine Creatinine: Creatinine Clearance Estimation of Urea Serum Proteins--Albumin: Globulin Ratio Serum Total Cholesterol Lipid Profile Serum Calcium and Phosphorus Serum Bilirubin Serum Transaminases Serum Alkaline Phosphatase Serum Uric Acid 4. Equipments And Procedures Point of Care Testing pH Meter Paper Chromatography and Thin Layer Chromatography Protein Electrophoresis Polyacrylamide Gel Electrophoresis Electrolyte Analysis Arterial Blood Gas Analyzer Enzyme-Linked Immunosorbent Assay Immunodiffusion Autoanalyzer DNA Isolation from Blood/Tissues 5. Function Tests Gastric Function Tests and Analysis of Gastric Juice Pancreatic Function Tests Liver Function Tests Analysis of Bile Renal Function Tests Thyroid Function Tests Adrenal Function Tests 6. Food and Energy Energy Content of Food and Glycemic Index Fats in Food 7. Clinical Case Studies Basis and Rationale of Biochemical Tests Done in Certain Clinical Conditions 8. Objective Structured Practical Examination Objective Structured Practical Examination 9. Reagents Reagent Preparation 10. Normal Values Normal Values Worksheet

Based on BHMS syllabus by CCH.This book deals with the basic knowledge of practical biochemistry but also its application in actual clinical practice.Creates an abiding interest in the practical aspects of the subject.

This is an ideal practical manual of biochemistry for MBBS students. It includes flowcharts, diagrams and colour pictures for clear visualization and understanding of the topics. Formulation of working reagents has been described along with each experiment. The manual includes viva-voce questions as well as information on biomedical waste segregations and disposal.