Methodologies and databases for biochemistry and molecular biology are included in this easy-to-use laboratory reference. Its logical presentation enables the reader to quickly and conveniently locate the information relevant to his or her needs. Featured are tables containing data on amino acids, proteins, nucleosides, nucleotides, and nucleic acids. Also featured are lipids and physical chemical data. Edited by a leading professional in the field, this compact, yet comprehensive bench manual serves as the definitive reference source for your laboratory.

This book analyzes the right pathway to solve the controversial identifications of some Trichoderma species on the basis of sampling procedures, slide culture techniques, macroscopic and microscopic analysis, and molecular tools. Most species of the genus Trichoderma grow rapidly in artificial culture and produce large numbers of small green or white conidia from conidiogenous cells located at the ends of conidiophores. The morphological characters are reported to be variable to a certain degree in their color, shape of conidia, conidiophore, pustules, and phialade. These characteristics allow a comparatively easy means of identification of Trichoderma as a genus but the species concept is difficult to deduce and there is considerable confusion over the application of specific names. This work provides an essential link between data and taxa as a means to verify the taxonomic characters of the strains sequenced, and macroscopic and microscopic characteristics. Otherwise, a species level identification study cannot be corrected or uncorrected, and the user has to rely on the person perhaps making a mis-identification.

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

This volume has evolved from a laboratory methods book that one of us first compiled nearly fifteen years ago. Since that time the book has undergone many minor revisions in order to include new methods and updated versions of older methods. The result has been an increasingly useful and more widely circulated book. However, the recent series of technological explosions generally lumped together under the name of "recombinant DNA technology" has been a turning point in the evolution of this previously underground publication. Minor revisions will no longer do. To keep the book useful we have had to make major revi sions and additions. The result is a dramatically expanded book that should be more useful to more people. The larger size and wider usefulness of the book have made this more formal publication seem a reasonable step to take. One of the reasons that this volume should be useful to many people is that it includes only procedures that have been used repeatedly by us and that have proven highly reliable both to ourselves and to others in our laboratories.

Introductory Practical Biochemistry, designed to cater to the requirements of students of biochemistry, microbiology, molecular biology, cellular biology etc. covers modern techniques employed for qualitative and quantitative analysis of biomolecules. The techniques for genetic transformation etc., have been included to give preliminary information to the beginners in the field of genetic engineering. Radioisotopic and immunological techniques also find a place in the book. Each chapter starts with introductory details of the techniques followed by simple laboratory exercises. The book provides concise information on theoretical and practical aspects of the techniques employed in biochemical studies for the Undergraduate and Postgraduate students, Instructors and Research workers.