Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protein, RNA, and DNA techniques, it will serve students, teachers, and researchers in plant physiology, biophysics, and animal molecular biology who have no previous experience handling recombinant DNA or purified proteins. It can also be used by the established molecular biologist who wishes to utilize the powerful techniques of recombinant DNA to explore the mysteries of the plant kingdom. - Eight basic experiments which can be used collectively or individually cover - Recombinant Cloning and Screening in E. coli; DNA Sequencing - Plant RNA Isolation and in Vitro Translations - Plant DNA Isolations and Genomic DNA Southern AnalysisChloroplast Isolation and Protein SynthesisPlant Tissue Culture and Agrobacterium Transformations - Experiments that have been student tested for three years - Blueprints for setting up gel rigs - Comprehensive course schedule outlining individual procedures to be finished in each lab segment - Course can be tailored to suit the needs of the individual instructor

Plant-specific protocols written in a clear and straightforward manner. Details modifications required before standard molecular biology techniques can be applied to plant material. Annotation copyrighted by Book News, Inc., Portland, OR

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment