Molecular Zoology Advances, Strategies, and Protocols Edited by Joan D. Ferraris and Stephen R. Palumbi Contemporary tools of molecular biology continue to open new areas of biological research and to provide important answers to classic problems. Zoological questions of mating strategies, physiological adaptation, genetic exchange between populations, cell lineages during development, and many others are now being powerfully addressed using tools from the molecular arsenal. To provide broad access to these tools requires an authoritative reference that highlights recent advances, lays out future strategies, and provides working protocols to a wide audience of zoological scientists. Molecular Zoology: Advances, Strategies, and Protocols outlines the core concepts of these critical molecular techniques and provides specific instructions for their use. The book is divided into two main parts: Research Strategies and Protocols. The first section features detailed descriptions of the research approaches that incorporate molecular tools in the study of developmental, physiological, ecological, and evolutionary processes. In addition to charting recent advances, this section shows how to interpret results and describes the advantages and disadvantages of alternative approaches. These chapters function as entry points to molecular zoology for broadly trained zoologists without formal molecular training, graduate students, and molecular biologists in other fields. The second section is a compilation of over 60 protocols which have been developed, tested, and perfected by leading researchers in the field. It provides step-by-step coverage of each protocol, featuring for each a summary of its underlying rationale, a list of necessary reagents and solutions, and a discussion of potential obstacles to a particular technique. Specific techniques covered in the book include: * Applications of parametric bootstrapping in molecular phylogenetics * Microsatellite analysis of genetic mating systems and genetic relatedness * Use of RAPD-PCR markers in genetic structure and genealogies * PCR-based cloning across large taxonomic distances * Cell lineage analysis using retroviral vectors * Osmoregulatory gene characterization and expression * Regulatory element identification and transcription factor analysis * Protocols for in situ hybridization, DNA footprinting, gene knockout, ribonuclease protection assay, and coupled transcription/translation reactions. Molecular Zoology: Advances, Strategies, and Protocols is an authoritative resource designed to provide both basic and in-depth explanations of molecular investigation procedures for research scientists in all areas of organismal and integrative biology, including zoology, marine biology, and ecology. With its extensive coverage of molecular protocols, graduate students in biology will also find this book to be an indispensable manual for laboratory work.

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Jean Octave Edmond Perrier was a French zoologist who lived through the tumult of British Darwinism and Lyellism, and reminds us in this revealing account that French scientists had much to contribute to such perennial topics as evolution, catastrophism and creationism. While very much a product of the Third Republic, Perrier’s account also aimed to outline timeless issues and permanent advances in taxonomic and developmental biology since classical Greece and Rome. In this aim he succeeds with surprisingly modern perspectives for a book first published in 1884. Perrier was born May 9, 1844 at Tulle, the son of the principal of a school which now bears his name, Lycée Edmond Perrier. In 1864 he was accepted to the École Normale Supérieure, where he was strongly influenced by Louis Pasteur and Henri de Lacaze-Duthiers. After working for three years at a high school in Agen, he obtained a post of naturalist-aid at the Muséum National d'Histoire Naturelle (1868), advancing in that institution to Chair of Natural History of Molluscs, Worms and Corals (1876–1903) and then Director of the museum (1900–1919) and Chair of Comparative Anatomy (1903–1921). Previous directors of the museum included many of the scientists he discusses in this book: George Cuvier (1822–1823, 1826–1827, 1830–1831), Isidore Geoffrey St Hilaire (1860– 1861), and Alphonse Milne-Edwards (1891–1900). Perrier’s own research on echinoderms and earthworms took him on several expeditions in 1880-1885, mostly to Atlantic and Mediterranean coasts, but also to the Caribbean.

I have been called upon to write illustrative sketches to a series of engravings, designed by an eminent artist. In performing my part of the work I have thrown the Mammalia into twenty-four groups--corresponding more or less to the picture designs--and have dwelt chiefly on the geographical distribution of the animals. The Cetaceae and Vespertilionidae are properly omitted. In the groups given there is no attempt made at any very scientific arrangement. The sketches are purely of a popular character, even the scientific nomenclature being avoided. It is hoped, however, that they may prove of service to the zoological tyro, and form as it were his first stepping-stone to a higher order of classification. In reality, notwithstanding the prodigious speculations of learned anatomists, no truly good arrangement of the Mammalia has yet been arrived at; the deficiency arising from the fact that, as yet, no true zoologist has had the opportunity of a sufficiently extended observation of the natural habits of animals. Mayne Reid