OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 249: Fish Cell Line Acute Toxicity - The RTgill-W1 cell line assay
Title | OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 249: Fish Cell Line Acute Toxicity - The RTgill-W1 cell line assay PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 51 |
Release | 2021-06-18 |
Genre | |
ISBN | 9264714138 |
The RTgill-W1 cell line assay describes a 24-well plate format fish cell line acute toxicity test using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. After 24 h of exposure to the test chemical, cell viability is assessed based on three fluorescent cell viability indicator dyes, measured on the same set of cells. Resazurin enters the cells in its non-fluorescent form and is converted to the fluorescent product, resorufin, by mitochondrial, microsomal or cytoplasmic oxidoreductases.
OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 203: Fish, Acute Toxicity Test
Title | OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 203: Fish, Acute Toxicity Test PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 24 |
Release | 2019-06-18 |
Genre | |
ISBN | 9264069968 |
The fish are exposed to the test substance preferably for a period of 96 hours. Mortalities are recorded at 24, 48, 72 and 96 hours and the concentrations which kill 50 per cent of the fish (LC50) are determined where possible. One or more species ...
OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 210: Fish, Early-life Stage Toxicity Test
Title | OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 210: Fish, Early-life Stage Toxicity Test PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 24 |
Release | 2013-07-26 |
Genre | |
ISBN | 9264203788 |
The test method described in this Test Guideline, is intended to define the lethal and sub-lethal effects of chemicals on the early life stages of the species tested. The early-life stages of fish are exposed to five concentrations of the test ...
OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 204: Fish, Prolonged Toxicity Test: 14-Day Study
Title | OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 204: Fish, Prolonged Toxicity Test: 14-Day Study PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 9 |
Release | 1984-04-04 |
Genre | |
ISBN | 9264069984 |
This Test Guideline presents guidance for measurement of lethal and other observed effects in fish exposed to test substances. It may be used in place of Test Guideline 203 if a longer observation period is considered useful and appropriate and the ...
OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 236: Fish Embryo Acute Toxicity (FET) Test
Title | OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 236: Fish Embryo Acute Toxicity (FET) Test PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 22 |
Release | 2013-07-26 |
Genre | |
ISBN | 9264203702 |
The test method described in this Test Guidelineis inteneded to determine the acute or letal toxicity of chemicals on embryonic stages of fish (Danio rerio). Newly fertilised zebrafish eggs are exposed to the test chemical for a period of 96 hrs ...
OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 212: Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages
Title | OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 212: Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 20 |
Release | 1998-09-21 |
Genre | |
ISBN | 9264070141 |
In this Short-term Toxicity Test on Fish Embryo and Sac-Fry Stages, the life stages from the newly fertilized egg to the end of the sac-fry stage are exposed. The embryo and sac-fry stages of fish are exposed to five concentrations of the test ...
OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 215: Fish, Juvenile Growth Test
Title | OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 215: Fish, Juvenile Growth Test PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 16 |
Release | 2000-01-21 |
Genre | |
ISBN | 9264070206 |
This Test Guideline is designed to assess the effects of prolonged exposure to chemicals on the growth of juvenile fish. Juvenile fish in exponential growth phase are placed, after being weighed, in test chambers and are normally exposed to five ...