This book focuses on biological engineering techniques, multi-omics big-data integration, and data-mining techniques, as well as cutting-edge researches in principles and applications of several synthetic biology applications. Synthetic biology is a new research area, while it has been rooted from the long-established area including biological engineering, metabolite engineering, and systems biology. This book will discuss the following aspects: (1) introduction to synthetic biology and iGEM, especially focusing on the systematic design, rational engineering, and sustainability of design in the omics ages; (2) synthetic biology–related multi-omics data integration and data mining techniques; (3) the technical issues, development issues, and safety issues of synthetic biology; (4) data resources, web services, and visualizations for synthetic biology; and (5) advancement in concrete research on synthetic biology, with several case studies shown. Devised as a book on synthetic biology research and education in the omics age, this book has put focuses on systematic design, rational engineering, and sustainability of design for synthetic biology, which will explain in detail and with supportive examples the “What,” “Why,” and “How” of the topic. It is an attempt to bridge the gap between synthetic biology’s research and education side, for best practice of synthetic biology and in-depth insights for the related questions.

This book details recent advances in the applications of nanobiofertilizers as a substitute for synthetic fertilizers in boosting food production. With the steady rise of the world’s population, there is a need to increase the production of safe and nutritious food. The constant loss of arable land, as a result of various anthropogenic activities from human action, has become a threat to global biodiversity and ecosystems. Additionally, the issue of climate change has imposed many obstacles to increasing agricultural productivity, especially from biotic and abiotic stressors and temperature-limited environments, such as in high altitudes or seasonally hot regions. Because of these factors, there is a need to adopt sustainable and modern technologies that can boost and improve the rate of food production. One of the cheapest means of enhancing sustainable food production is to explore natural and unlimited beneficial microorganisms, particularly those that can increase the level of soil fertility, improve crop production and health, improve tolerance to stress, support nutrient uptake and availability, and boost natural biodiversity. The synergetic effect of nanotechnology and beneficial microorganisms for the effective bio-fabrication of nanobiofertilizers, is a sustainable solution for producing pesticide-free food. This book provides a deep insight into microbial diversity, recent techniques used for the isolation, screening, and characterization of beneficial microorganisms with eco-friendly attributes, used for bioengineering of nanobiofertilizers, as well as the application of proteomics, metabolomics, genomics, and bioinformatics. The book also covers commercialization, patents, and the business and socio-economic aspects of nanobiofertilizers, as well as the role of policymakers, stakeholders, and government agencies in the translation of nanobioferilizer research into policy. Audience The book is a useful resource for a diverse audience, including industrialists, food industry professionals, agriculturists, agricultural microbiologists, plant pathologists, botanists, microbiologists, biotechnologists, nanotechnologists, microbial biotechnologists, farmers, policymakers, and extension workers.

Algae are sunlight-driven cell factories, and can efficiently absorb CO2 and convert light energy to chemical energy such as lipid, starch and other carbohydrates and release O2. Algal feedstock is a promising resource for bioproduct production, given its high photosynthetic efficiency for producing biomass compared to conventional crops. Microalgae can be used for flue-gas and wastewater bioremediation. This book highlights recent breakthroughs in the multidisciplinary areas of algal biotechnology and the chapters feature recent developments from cyanobacteria to eukaryotic algae, from theoretical biology to applied biology. It also includes the latest advancements in algal-based synthetic biology, including metabolic engineering, artificial biological system construction and green chemicals production. With contributions by leading authorities in algal biotechnology research, it is a valuable resource for graduate students and researchers in the field, and those involved in the study of photosynthesis and green-cell factories.

Microbial Cell Factories Engineering for Production of Biomolecules presents a compilation of chapters written by eminent scientists worldwide. Sections cover major tools and technologies for DNA synthesis, design of biosynthetic pathways, synthetic biology tools, biosensors, cell-free systems, computer-aided design, OMICS tools, CRISPR/Cas systems, and many more. Although it is not easy to find relevant information collated in a single volume, the book covers the production of a wide range of biomolecules from several MCFs, including Escherichia coli, Bacillus subtilis, Pseudomonas putida, Streptomyces, Corynebacterium, Cyanobacteria, Saccharomyces cerevisiae, Pichia pastoris and Yarrowia lipolytica, and algae, among many others. This will be an excellent platform from which scientific knowledge can grow and widen in MCF engineering research for the production of biomolecules. Needless to say, the book is a valuable source of information not only for researchers designing cell factories, but also for students, metabolic engineers, synthetic biologists, genome engineers, industrialists, stakeholders and policymakers interested in harnessing the potential of MCFs in several fields. - Offers basic understanding and a clear picture of various MCFs - Explains several tools and technologies, including DNA synthesis, synthetic biology tools, genome editing, biosensors, computer-aided design, and OMICS tools, among others - Harnesses the potential of engineered MCFs to produce a wide range of biomolecules for industrial, therapeutic, pharmaceutical, nutraceutical and biotechnological applications - Highlights the advances, challenges, and future opportunities in designing MCFs

Process Control, Intensification, and Digitalisation in Continuous Biomanufacturing Explore new trends in continuous biomanufacturing with contributions from leading practitioners in the field With the increasingly widespread acceptance and investment in the ??technology, the last decade has demonstrated the utility of continuous ??processing in the pharmaceutical industry. In Process Control, Intensification, and Digitalisation in Continuous Biomanufacturing, distinguished biotechnologist Dr. Ganapathy Subramanian delivers a comprehensive exploration of the potential of the continuous processing of biological products and discussions of future directions in advancing continuous processing to meet new challenges and demands in the manufacture of therapeutic products. A stand-alone follow-up to the editor’s Continuous Biomanufacturing: Innovative Technologies and Methods published in 2017, this new edited volume focuses on critical aspects of process intensification, process control, and the digital transformation of biopharmaceutical processes. In addition to topics like the use of multivariant data analysis, regulatory concerns, and automation processes, the book also includes: Thorough introductions to capacitance sensors to control feeding strategies and the continuous production of viral vaccines Comprehensive explorations of strategies for the continuous upstream processing of induced microbial systems Practical discussions of preparative hydrophobic interaction chromatography and the design of modern protein-A-resins for continuous biomanufacturing In-depth examinations of bioprocess intensification approaches and the benefits of single use for process intensification Perfect for biotechnologists, bioengineers, pharmaceutical engineers, and process engineers, Process Control, Intensification, and Digitalisation in Continuous Biomanufacturing is also an indispensable resource for chemical engineers seeking a one-stop reference on continuous biomanufacturing.