Cryopreservation has proven to be an important tool for the storage and conservation of plant genetic resources. This book is a unique resource for plant scientists, providing more than 100 ready-to-use cryopreservation protocols for plant types from algae and bryophytes to a range of flowering plants. It includes techniques for diverse plant parts such as dormant buds, pollen, and apical meristems and for cell types such as suspension and callus cultures.

Since the first edition of our book "Tissue Culture in Fores try" in 1982 we have witnessed remarkable advances in cell and tissue culture technologies with woody perennials. In addition to forest biologists in government, industry, and universities, we now have molecular biologists, genetic engineers, and biochemists using cell and tissue cultures of woody species routinely. There fore, the time has come for an update of the earlier edition. In our present effort to cover new developments we have expanded to three volumes: 1. General principles and Biotechnology 2. Specific Principles and Methods: Growth and Development 3. Case Histories: Gymnosperms, Angiosperms and Palms The scientific barriers to progress in tree improvement are not so much lack of foreign gene expression in plants but our current inabili ty to regenerate plants in true-to-type fashion on a mas sive and economic scale. To achieve this in the form of an appro pr iate biotechnology, cell and tissue culture will increasing ly require a better understanding of basic principles in chemistry and physics that determine structural and functional relationships among molecules and macromolecules (proteins, RNA, DNA) within cells and tissues. These principles and their relationship with the culture medium and its physical environment, principles of clonal propagation, and genetic variation and ultrastructure are discussed in volume one.

This book constitutes the refereed proceedings of the 17th Conference on Artificial Intelligence in Medicine, AIME 2019, held in Poznan, Poland, in June 2019. The 22 revised full and 31 short papers presented were carefully reviewed and selected from 134 submissions. The papers are organized in the following topical sections: deep learning; simulation; knowledge representation; probabilistic models; behavior monitoring; clustering, natural language processing, and decision support; feature selection; image processing; general machine learning; and unsupervised learning.

Gendered Lives: Intersectional Perspectives, Seventh Edition, is an interdisciplinary text-reader that provides an introduction to women's and gender studies within a global context by examining the diversity of US women's lives across categories of race-ethnicity, class, sexuality, gender expression, disability, age, and immigration status. Substantial chapter introductions provide statistical information and explanations of key concepts and ideas as a context for the reading selections. Each chapter includes reading questions and suggestions for taking action, to help students link what they learn to their own lives and to the world around them.

How, asks James E. Strick, could spontaneous generation--the idea that living things can suddenly arise from nonliving materials--come to take root for a time (even a brief one) in so thoroughly unsuitable a field as British natural theology? No less an authority than Aristotle claimed that cases of spontaneous generation were to be observed in nature, and the idea held sway for centuries. Beginning around the time of the Scientific Revolution, however, the doctrine was increasingly challenged; attempts to prove or disprove it led to important breakthroughs in experimental design and laboratory techniques, most notably sterilization methods, that became the cornerstones of modern microbiology and sped the ascendancy of the germ theory of disease. The Victorian debates, Strick shows, were entwined with the public controversy over Darwin's theory of evolution. While other histories of the debates between 1860 and 1880 have focused largely on the experiments of John Tyndall, Henry Charlton Bastian, and others, Sparks of Life emphasizes previously understudied changes in the theories that underlay the debates. Strick argues that the disputes cannot be understood without full knowledge of the factional infighting among Darwinians themselves, as they struggled to create a socially and scientifically viable form of Darwinian science. He shows that even the terms of the debate, such as biogenesis, usually but incorrectly attributed to Huxley, were intensely contested.