This updated and up-to-date version of the first edition continues with the really interesting stuff to spice up a standard biophysics and biophysical chemistry course. All relevant methods used in current cutting edge research including such recent developments as super-resolution microscopy and next-generation DNA sequencing techniques, as well as industrial applications, are explained. The text has been developed from a graduate course taught by the author for several years, and by presenting a mix of basic theory and real-life examples, he closes the gap between theory and experiment. The first part, on basic biophysical chemistry, surveys fundamental and spectroscopic techniques as well as biomolecular properties that represent the modern standard and are also the basis for the more sophisticated technologies discussed later in the book. The second part covers the latest bioanalytical techniques such as the mentioned super-resolution and next generation sequencing methods, confocal fluorescence microscopy, light sheet microscopy, two-photon microscopy and ultrafast spectroscopy, single molecule optical, electrical and force measurements, fluorescence correlation spectroscopy, optical tweezers, quantum dots and DNA origami techniques. Both the text and illustrations have been prepared in a clear and accessible style, with extended and updated exercises (and their solutions) accompanying each chapter. Readers with a basic understanding of biochemistry and/or biophysics will quickly gain an overview of cutting edge technology for the biophysical analysis of proteins, nucleic acids and other biomolecules and their interactions. Equally, any student contemplating a career in the chemical, pharmaceutical or bio-industry will greatly benefit from the technological knowledge presented. Questions of differing complexity testing the reader's understanding can be found at the end of each chapter with clearly described solutions available on the Wiley-VCH textbook homepage under: www.wiley-vch.de/textbooks

"Biophysical Chemistry explores the concepts of physical chemistry and molecular structure that underlie biochemical processes. Ideally suited for undergraduate students and scientists with backgrounds in physics, chemistry or biology, it is also equally accessible to students and scientists in related fields as the book concisely describes the fundamental aspects of biophysical chemistry, and puts them into a biochemical context. This second edition has been fully updated throughout with novel techniques, with a new chapter on advances in cryo-electron microscopy and exciting new content throughout on big data techniques, structural bioinformatics, systems biology and interaction networks, and artificial intelligence and machine learning. The book is organized in four parts, covering thermodynamics, kinetics, molecular structure and stability, and biophysical methods. Cross-references within and between these parts emphasize common themes and highlight recurrent principles. End of chapter problems illustrate the main points explored and their relevance for biochemistry, enabling students to apply their knowledge and to transfer it to laboratory projects"--

Incorporating dramatic recent advances, "Methods in Modern Biophysics" presents a fresh and timely introduction to modern biophysical methods. This innovative text surveys and explains the ten key biophysical methods, including those related to biophysical nanotechnology, scanning probe microscopy, X-ray crystallography, ion mobility spectrometry, mass spectrometry, and proteomics. Containing much information previously unavailable in tutorial form, "Methods in Modern Biophysics" employs worked examples and more than 260 illustrations to fully detail the techniques and their underlying mechanisms. The book was written for advanced undergraduate and graduate students, postdocs, researchers, lecturers and professors in biophysics, biochemistry, general biology and related fields.

This book provides an introduction to physical chemistry that is directed toward applications to the biological sciences. Advanced mathematics is not required. This book can be used for either a one semester or two semester course, and as a reference volume by students and faculty in the biological sciences.

"Biophysical Chemistry is an outstanding book that delivers both fundamental and complex biophysical principles, along with an excellent overview of the current biophysical research areas, in a manner that makes it accessible for mathematically and non-mathematically inclined readers." (Journal of Chemical Biology, February 2009) This text presents physical chemistry through the use of biological and biochemical topics, examples and applications to biochemistry. It lays out the necessary calculus in a step by step fashion for students who are less mathematically inclined, leading them through fundamental concepts, such as a quantum mechanical description of the hydrogen atom rather than simply stating outcomes. Techniques are presented with an emphasis on learning by analyzing real data. Presents physical chemistry through the use of biological and biochemical topics, examples and applications to biochemistry Lays out the necessary calculus in a step by step fashion for students who are less mathematically inclined Presents techniques with an emphasis on learning by analyzing real data Features qualitative and quantitative problems at the end of each chapter All art available for download online and on CD-ROM

Peter Atkins and Julio de Paula offer a fully integrated approach to the study of physical chemistry and biology.