Volume 2.

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers microbial metagenomics, metatranscriptomics, and metaproteomics, and includes chapters on such topics as in-solution FISH for single cell genome preparation, preparation of BAC libraries from marine microbial community DNA, and preparation of microbial community cDNA for metatranscriptomic analysis in marine plankton.

Methods in Microbiology

Dr. Joshua Lederberg - scientist, Nobel laureate, visionary thinker, and friend of the Forum on Microbial Threats - died on February 2, 2008. It was in his honor that the Institute of Medicine's Forum on Microbial Threats convened a public workshop on May 20-21, 2008, to examine Dr. Lederberg's scientific and policy contributions to the marketplace of ideas in the life sciences, medicine, and public policy. The resulting workshop summary, Microbial Evolution and Co-Adaptation, demonstrates the extent to which conceptual and technological developments have, within a few short years, advanced our collective understanding of the microbiome, microbial genetics, microbial communities, and microbe-host-environment interactions.

Based on the thesis that insights into both evolution and ecology can be obtained through the study of microorganismsm, Microbial Ecology examines microbiology through the lens of evolutionary ecology. Measured from a microbial perspective, this text covers such topics as optimal foraging, genome, reduction, novel evolutionary mechanisms, bacterial speciation, and r and K selection. Numerous aspects of microbial existence are also discussed and include: species competition, predation, parasitism, mutualism, microbial communication through quorum sensing and other. The result is a context for understanding microbes in nature and a framework for microbiologists working in industry, medicine, and the environment. - Applies evolutionary ecological concepts to microbes - Addresses individual, population and community ecology - Presents species concepts and offers insights on the origin of life and modern microbial ecology - Examines topics such as species interactions, nutrient cycling, quorum sensing and cheating

Marine biological science is now studied at the molecular level and although research scientists depend on information gained using molecular techniques, there is no book explaining the philosophy of this approach. Molecular Approaches to the Study of the Ocean introduces the reasons why molecular technology is such a powerful tool in the study of the oceans, describing the types of techniques that can be used, why they are useful and gives examples of their application. Molecular biological techniques allow phylogenetic relationships to be explored in a manner that no macroscopic method can; although the book deals with organisms near the base of the marine food web, the ideas can be used in studies of macroorganisms as well as those in freshwater environments.

FROM THE INTRODUCTION This three-volume set, Bioremediation: Principles and Practice, provides state of the art description of advances in pollution treatment and reduction using biological means; identify and address, at a fundamental level, broad scientific and technological areas that are unique to the subject or theme and that must be understood if advances are to be made; and provide a comprehensive overview of new developments at the regulatory, desk-top, bench-scale, pilot scale, and full-scale levels. The set covers all media-air, water, and soil/sediment-and blends the talents, knowledge, and know-how of academic, industrial, governmental, and international contributors. The set addresses the removal of both hazardous and nonhazardous contaminants from the liquid, solid, and gas phase using biological processes. This includes the biological treatment of wastes of municipal and industrial origin; bioremediation of leachates, soils, and sediments; and biofiltration for contaminated gases.