1. General General Instructions Laboratory Equipments and Procedures Buffers and pH Quality Control 2. Analysis of Urine and Cerebrospinal Fluid Normal Urine: Characteristics and Analysis Abnormal Constituents of Urine Screening of Urine for Inborn Errors of Metabolism and the Use of Paper Chromatography Composition of Cerebrospinal Fluid 3. Quantitative Experiments Principles of Colorimetry Principles of Spectrophotometer Estimation of Glucose Glucose Tolerance Test Estimation of Serum and Urine Creatinine: Creatinine Clearance Estimation of Urea Serum Proteins--Albumin: Globulin Ratio Serum Total Cholesterol Lipid Profile Serum Calcium and Phosphorus Serum Bilirubin Serum Transaminases Serum Alkaline Phosphatase Serum Uric Acid 4. Equipments And Procedures Point of Care Testing pH Meter Paper Chromatography and Thin Layer Chromatography Protein Electrophoresis Polyacrylamide Gel Electrophoresis Electrolyte Analysis Arterial Blood Gas Analyzer Enzyme-Linked Immunosorbent Assay Immunodiffusion Autoanalyzer DNA Isolation from Blood/Tissues 5. Function Tests Gastric Function Tests and Analysis of Gastric Juice Pancreatic Function Tests Liver Function Tests Analysis of Bile Renal Function Tests Thyroid Function Tests Adrenal Function Tests 6. Food and Energy Energy Content of Food and Glycemic Index Fats in Food 7. Clinical Case Studies Basis and Rationale of Biochemical Tests Done in Certain Clinical Conditions 8. Objective Structured Practical Examination Objective Structured Practical Examination 9. Reagents Reagent Preparation 10. Normal Values Normal Values Worksheet

This is an ideal practical manual of biochemistry for MBBS students. It includes flowcharts, diagrams and colour pictures for clear visualization and understanding of the topics. Formulation of working reagents has been described along with each experiment. The manual includes viva-voce questions as well as information on biomedical waste segregations and disposal.

This book will serve as a practical manual for undergraduate students in MBBS. Related clinical concepts will also be useful in the preparation of postgraduate entrance exams. This book will serve as a practical manual for undergraduate students in MBBS. Related clinical concepts will also to useful in the preparation of Post-graduate entrance exams.

The seventh edition of this book is a comprehensive guide to biochemistry for medical students. Divided into six sections, the book examines in depth topics relating to chemical basics of life, metabolism, clinical and applied biochemistry, nutrition, molecular biology and hormones. New chapters have been added to this edition and each chapter includes clinical case studies to help students understand clinical relevance. A 274-page free booklet of revision exercises (9789350906378), providing essay questions, short notes, viva voce and multiple choice questions is included to help students in their exam preparation. Free online access to additional clinical cases, key concepts and an image bank is also provided. Key points Fully updated, new edition providing students with comprehensive guide to biochemistry Includes a free booklet of revision exercises and free online access Highly illustrated with nearly 1500 figures, images, tables and illustrations Previous edition published in 2010

Fully revised, new edition presenting latest developments in medical biochemistry. Includes many new chapters and case reports. Previous edition published in 2006.

Unit 1: Introduction of Clinical Biochemistry 1. Laboratory Apparatus and Equipment, Good and Safe Laboratory Practice, and Waste Disposal Systems in Laboratory Unit 2: Qualitative Experiments and their Clinical Applications 1. Analysis of Carbohydrates 2. Analysis of Proteins 3. Analysis of Physical and Chemical Composition of Physiological Urine 4. Identify, Perform and Interpret Pathological Urine Analysis and Correlate it with Pathological States Unit 3: Quantitative Experiments and their Clinical Interpretation 1. Principle of Colorimetry 2. Principle of Spectrophotometry 3. Estimation of Blood Glucose 4. Glucose Tolerance Test and Glycated Hemoglobin 5. Liver Function Test 6. Kidney Function Test 7. Lipid Profile (Atherogenic Profile) 8. Estimation of Serum Calcium and Serum Phosphorus Unit 4: Self-Directed Learning Exercises 1. pH Meter 2. Water Homeostasis and Estimation of Na+ and K+ with ISE Analyzer 3. Arterial Blood Gas Analyzer 4. Chromatography 5. Electrophoresis 6. Enzyme-linked Immunosorbent Assay 7. Antigen-Antibody Interaction (Immunodiffusion) 8. Quality Control in Clinical Laboratory 9. DNA Isolation from Blood and Tissue Unit 5: Early Clinical Exposure Exercises and Reflective Writing 1. Analysis of Cerebrospinal Fluid 2. Thyroid Function Test 3. Pancreatic Function Tests 4. Disorders of Acid-Base Balance Unit 6: Attitude, Ethics and Communication (AETCOM) Modules 1. Introduction of Clinical Methods 2. What does it Mean to be a Doctor? 3. What does it Mean to be a Patient? 4. The Doctor-Patient Relationship 5 The Foundations of Communications Unit 7: Biochemical Calculations and Reference Range 1. Preparations of Buffers and Solutions 2. Reference Value of Various Biochemical Parameters Integration with Medicine Unit 8: Practical Spots in Biochemistry 1. Practical Spots in Biochemistry Unit 9: Competency-Based Assessment for Practical Biochemistry 1. Competency-based Assessment for Practical Biochemistry Index