Visionary loner Jay Chevalier develops a revolutionary cell phone, pulsing with a living, biogenetic core, that offers free calls with untraceable privacy, completely negating established business models. LiveCell gobbles market share and initiates a grassroots movement of social change, but the pushback from the communications industry is swift and ruthless. Jay’s invention will change the world—if he can stay alive. Nonstop action, ill-destined love, and a tragically relevant plea for social change will propel you through this thriller at neural-network speed.

This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Imaging and spectroscopy are the most important and challenging techniques for not only research on materials science, chemistry, and biology, but also medical diagnoses. In this book, we have collected information on several novel imaging and spectroscopic techniques, including time-resolved electron diffraction/microscopy for materials science, various spectroscopes for physics and chemistry, and high-resolution computed tomography for medical science. We think that the content in each chapter is impressive and we hope this book will contribute to future instrument developments and new applications.

Optical Microscopy: Emerging Methods and Applications covers recent technical advances and new approaches to monitoring and altering cell physiology, examining membrane cytoarchitecture, observing multiple cellular activities and intact organ physiology, plus confocal imaging of live cell function, lifetime imaging, and automated clinical imaging cytometry. The book provides the reader with a synopsis of the most recent technical developments in optical microscopy as applied to scientific research. Each chapter introduces new methods by describing how these overcome limitations inherent in previous techniques. Software, hardware, and other equipment concerns are covered. Additionally, the book reviews current applications in order to stimulate future developments in optical microscopy, encouraging novel uses and new technical advances - Caged compounds, fluorescence ratio imaging, and CCD video cameras - Simultaneous multiple detection and real-time fluorescence microscopy - Simultaneous DIC and quantitative LLF video imaging - Total internal reflectance, time-resolved, and automated fluorescence microscopy - Laser-scanning confocal microscopy - Imaging for calcium measurements, membranes, glycoproteins, living cells, and cancer cells

This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.

A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.