This short text is designed to provide basic information about plant and microbial lipids not only for scientists working in the microbiological and plant fields, but for anyone wanting a concise introduction to this aspect of lipid biochemistry. We have long been aware that standard biochemistry books tend to. concentrate (sometimes exclusively) on animal lipids, thus neglecting many of the important and special features of other organisms. It is not our intention that the book should be comprehensive and we have not, for instance, provided complete lists of lipid compositions of all plants and bacterial species; a number of excellent specialist texts exist and many of these are listed for further reading. Instead we have sought to provide sufficient information for an advanced undergraduate or a research student to give them a 'feel' for the subject. By a combination of generalisation and the use of examples of special interest we hope the book will whet the appetite of the reader so that, by their own research, they are stimulated to discover and, perhaps, answer some of the fascinating questions concerning plant and microbial lipids. We trust that we shall succeed in these aims, even if that will mean more competition for research funds in our own fields! J. L. HARWOOD N. J. RUSSELL November 1983 Acknowledgements Our research careers have been devoted to a study of lipids: we have no regrets and are happy to acknowledge Professors J. N.

Concise chapters, written by experts in the field, cover a wide spectrum of topics on lipid and membrane formation in microbes (Archaea, Bacteria, eukaryotic microbes).All cells are delimited by a lipid membrane, which provides a crucial boundary in any known form of life. Readers will discover significant chapters on microbial lipid-carrying biomolecules and lipid/membrane-associated structures and processes.

Cell membranes are the initial and focal sites of stimulus perception and signal transduction. Membrane lipids are rich sources for the production of signaling messengers that mediate plant growth, development, and response to nutrient status and stresses. In recent years, substantial progress has been made toward understanding lipid signaling in plants, but many fundamental questions remain: What lipids are signaling messengers or mediators in plants? How are the signaling lipids produced and metabolized? In what plant cellular and physiological processes are various lipid mediators involved? How do they carry out their signaling functions? How do lipid signaling networks contribute to modulating plant growth, development, and responses to hormones and stresses? In this Research Topic issue, we invite the broad plant community to address the above questions.Cell membranes are the initial and focal sites of stimulus perception and signal transduction. Membrane lipids are rich sources for the production of signaling messengers that mediate plant growth, development, and response to nutrient status and stresses. In recent years, substantial progress has been made toward understanding lipid signaling in plants, but many fundamental questions remain: What lipids are signaling messengers or mediators in plants? How are the signaling lipids produced and metabolized? In what plant cellular and physiological processes are various lipid mediators involved? How do they carry out their signaling functions? How do lipid signaling networks contribute to modulating plant growth, development, and responses to hormones and stresses? In this Research Topic issue, we invite the broad plant community to address the above questions.

Selected Papers from an International Symposium held at East Lansing, Michigan, USA, May 1993

As scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In Plant Lipid Signaling Protocols, expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied. These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals.

A comprehensive handbook outlining state-of-the-art analytical techniques used in geomicrobiology, for advanced students, researchers and professional scientists.