This volume looks at all aspects of manipulation of Leptospira spp. from strain isolation to the latest techniques used to study the pathogenesis of leptospirosis. The chapters in this book cover topics such as the procedure to cultivate and isolate leptospires from both clinical and environmental samples; using methods like whole genome sequencing and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry to identify bacterial species; tools for gene inactivation and in vitro and in vivo assays to study the pathogenesis of leptospirosis; and the use of hamsters to evaluate leptospiral virulence and vaccine candidates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Leptospira spp.: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field and these fascinating organisms.

This volume covers all aspects of infection by pathogenic Leptospira species, the causative agents of the world’s most widespread zoonosis. Topics include aspects of human and animal leptospirosis as well as detailed analyses of our current knowledge of leptospiral structure and physiology, epidemiology, pathogenesis, genomics, immunity and vaccines. Updates are presented on leptospiral systematics, identification and diagnostics, as well as practical information on culture of Leptospira. Contact information is also provided for Leptospira reference centers. All chapters were written by experts in the field, providing an invaluable reference source for scientists, veterinarians, clinicians and all others with an interest in leptospirosis.

THE ESSENTIAL WORK IN TRAVEL MEDICINE -- NOW COMPLETELY UPDATED FOR 2018 As unprecedented numbers of travelers cross international borders each day, the need for up-to-date, practical information about the health challenges posed by travel has never been greater. For both international travelers and the health professionals who care for them, the CDC Yellow Book 2018: Health Information for International Travel is the definitive guide to staying safe and healthy anywhere in the world. The fully revised and updated 2018 edition codifies the U.S. government's most current health guidelines and information for international travelers, including pretravel vaccine recommendations, destination-specific health advice, and easy-to-reference maps, tables, and charts. The 2018 Yellow Book also addresses the needs of specific types of travelers, with dedicated sections on: · Precautions for pregnant travelers, immunocompromised travelers, and travelers with disabilities · Special considerations for newly arrived adoptees, immigrants, and refugees · Practical tips for last-minute or resource-limited travelers · Advice for air crews, humanitarian workers, missionaries, and others who provide care and support overseas Authored by a team of the world's most esteemed travel medicine experts, the Yellow Book is an essential resource for travelers -- and the clinicians overseeing their care -- at home and abroad.

Interpretation of Equine Laboratory Diagnostics offers a comprehensive approach to equine laboratory diagnostics, including hematology, clinical chemistry, serology, body fluid analysis, microbiology, clinical parasitology, endocrinology, immunology, and molecular diagnostics. Offers a practical resource for the accurate interpretation of laboratory results, with examples showing real-world applications Covers hematology, clinical chemistry, serology, body fluid analysis, microbiology, clinical parasitology, endocrinology, immunology, and molecular diagnostics Introduces the underlying principles of laboratory diagnostics Provides clinically oriented guidance on performing and interpreting laboratory tests Presents a complete reference to establish and new diagnostic procedures Offers a practical resource for the accurate interpretation of laboratory results, with examples showing real-world applications Covers hematology, clinical chemistry, serology, body fluid analysis, microbiology, clinical parasitology, endocrinology, immunology, and molecular diagnostics Introduces the underlying principles of laboratory diagnostics Provides clinically oriented guidance on performing and interpreting laboratory tests Presents a complete reference to established and new diagnostic procedures

Bacterial Pathogens and their Virulence Factors contains a detailed description of 32 major bacterial pathogens that affect human health and their associated virulence determinants. Chapter 1 gives an overview of the different types and classes of general virulence factors involved in host cell adherence and invasion, dissemination within the host, host cell damage, and evasion of host defense systems, as well as mechanisms by which these virulence factors are regulated. Chapters 2 through 33 give concise descriptions of the disease states associated with the 32 bacterial genera and their major pathogenic species, along with an in-depth description of the individual virulence factors that have been found to be functionally involved in pathogenicity. A detailed bibliography derived from primary literature and review articles accompanies each of these chapters, allowing the reader to delve more deeply into individual pathogens and their virulence determinants. Chapter 34 discusses the exciting possibilities and initial successes of using detailed information on a pathogen’s virulence toolkit to design new therapeutics aimed at specific virulence traits.

Diagnostic Pathology: Bone was designed for practicing pathologists who need access to up-to-date, comprehensive, and concise bone pathology knowledge in one convenient place. It includes the latest diagnostic information in this challenging subspecialty, while its unique image collection serves as an exceptional educational aid. Authored and updated by experts in the field, Diagnostic Pathology: Bone will be an essential guide to understanding bone tumor pathology and diagnosis. Features quick-reference test data tables and diagnoses of various bone and cartilage tumor types Templated page layout, bulleted text, and a generous number of high-quality images for point-of-care clinical reference Stunning image collection includes gross pathology, histopathology, immunohistochemistry, FISH, and correlative radiologic imaging to serve as an exceptional educational aid Brand-new chapters covering Ewing-like sarcomas with specific translocations, other types of vascular tumors of bone (new entities), and tumors of the synovium Includes new molecular pathology diagnostic information for bone tumors and new immunohistochemical antigens to identify bone pathologies

As the field of clinical microbiology continues to change, this edition of the Manual of Clinical Microbiology has been revised and rewritten to incorporate the most current clinical and laboratory information. In two volumes, 11 sections, and 152 chapters, it offers accessible and authoritative descriptions of important diseases, laboratory diagnosis, and therapeutic testing of all clinically significant bacteria, viruses, fungi, and parasites.