Initial identification of common genera; Gram-negative bacteria; Agrobacterium; Erwinia and pantoea; Erwinia amylovora group; Erwinia soft rot group; Pantoea; Pseudomonas; Acidovorax and xylophilus; Buirkholderia; Ralstonia; Xanthomonas; Xylella fastidiosa; Rhizomonas suberifaciens; Gram-positive bacteria; Streptomyces; Bacillus; Clostridium; Fastidious phloem-limited bacteria; Cell-wall free bacteria; Molecular techniques; DNA isolation procedure; Serological techniques; Automated techniques.

Laboratory Techniques in Plant Bacteriology is ideal for scientists and students who seek a career in plant pathogenic bacteria. This book contains 41 chapters comprising practicable techniques from isolation of bacterial plant pathogens to their identification up to species and race/biotype level. It includes identification protocols of morphological, biochemical, immunological, and molecular-based techniques. This book comprises all technological aspects of plant bacteriological studies. Its content is ideal for graduate students and research scholars including bacteriological professionals or technicians. The book ultimately provides working technologies useful for controlling bacterial disease pathogens.

Identification schemes; Gram-negative bacteria; Gram-positive bacteria; Cell wall-free prokaryotes.

This Laboratory Manual has been designed for students for easy understanding of basic plant pathological laboratory techniques related with Isolation of pathogen. Preservation of disease sample, Demonstration of Koch.s postutlates. Study of different groups of fungicides and antibiotics. Preparation of fungicides. Methods of application of fungicides. Bio-assay of fungicides, Bio control of plant pathogens and Identification of some important fungal pathogens. The book is fully colour book with digitized images have been made to identify diseases and pathogens with explanations of new terminologies to enhance students understanding about the subject. The book will be useful to beginners, students, instructors, scientists and research workers in the field of Plant Pathology and Agricultural Microbiology.

Media and nutrient solutions used by plant. Desinfection and sterilization: sterilization of laboratory. Isolation of bacteriophage and plant pathogenic. Diagnosing the causes of plant diseases. Increase of inoculum. Establishment of disease: inoculation, infection. Preservation of microorganisms. Microscopic techniques. Writing for publication.