JOHNSTONE COUNTRY. WHERE THE WILL DEFIES FEAR. They call him “The Rig Warrior.” Name: Barry Rivers. Occupation: Long haul trucker. Special skills: Defender of freedom. Patriot. Government sanctioned killer. A NATION OFF THE RAILS No one saw the first attack coming. A perfectly orchestrated assault on a mass-transit railroad line that left countless Americans dead. Then came more attacks. More rail systems sabotaged. More civilian lives lost. Intelligence experts are convinced this is no ordinary terrorist attack. To pull off something like this, it would take a deep-state traitor with dark foreign connections. And to stop them, it will take someone who isn’t afraid to shed blood. A HERO OFF THE GRID Enter Barry Rivers, the Rig Warrior. An urban legend in the intelligence community, Rivers has been living off the radar for years. But when he sees his country under attack, he reaches out to his son in the FBI to track down the enemies in our own government. To these high-ranking traitors, Rivers is a threat to their global agenda. But when Rivers revs up his tricked-out 18-wheeler—and goes after a runaway train on a collision course with disaster—all bets are off. The war is on. And with Barry Rivers at the wheel, it’s going to be the ultimate knockdown, drag-out fight for America’s future . . . Live Free. Read Hard.

Knit neat items inspired by sassy roller derby girls with the thirty hip knitting patterns that you will find in Knockdown Knits: 30 Projects from the Roller Derby Track. Roller derby, with its cheeky players, fast-paced jams, heart-stopping pileups, and rowdy, raucous atmosphere, is just as hot as the designs in this unconventional book. The Naptown Roller Girls of Indianapolis, Indiana, share the patterns they use when they’re knitting in the off-time. On skates, they're hell on wheels; in their spare time, they've discovered that knitting is a relaxing, creative outlet.

Jacobia “Jake” Tiptree is deep in her latest home improvement project—repainting the porch of her lovingly restored house in Eastport, Maine—when she notices the man repeatedly biking past her house. His face is unsettlingly familiar, but his chilling message seems inexplicable: Blood shows up again. Murder will out. Then there’s the anonymous email warning her to beware the Fourth of July—just two days away. Jake remembers some shady characters from her days as a hotshot financial manager, but she’s baffled as to the identity of her nemesis—until she receives a photo of a murdered man. From home invasion to kidnapping, this twisted killer is slowly but surely plotting a knockdown, not just for Jake but for the entire town of Eastport. BONUS: This edition includes an excerpt from Sarah Graves's Dead Level.

Since the discovery of the Warburg effect in the 1920s cancer has been tightly associated with the genetic and metabolic state of the cell. One of the hallmarks of cancer is the alteration of the cellular metabolism in order to promote proliferation and undermine cellular defense mechanisms such as apoptosis or detection by the immune system. However, the strategies by which this is achieved in different cancers and sometimes even in different patients of the same cancer is very heterogeneous, which hinders the design of general treatment options. Recently, there has been an ongoing effort to study this phenomenon on a genomic scale in order to understand the causality underlying the disease. Hence, current “omics” technologies have contributed to identify and monitor different biological pieces at different biological levels, such as genes, proteins or metabolites. These technological capacities have provided us with vast amounts of clinical data where a single patient may often give rise to various tissue samples, each of them being characterized in detail by genomescale data on the sequence, expression, proteome and metabolome level. Data with such detail poses the imminent problem of extracting meaningful interpretations and translating them into specific treatment options. To this purpose, Systems Biology provides a set of promising computational tools in order to decipher the mechanisms driving a healthy cell’s metabolism into a cancerous one. However, this enterprise requires bridging the gap between large data resources, mathematical analysis and modeling specifically designed to work with the available data. This is by no means trivial and requires high levels of communication and adaptation between the experimental and theoretical side of research.

The flexible filamentous plant viruses are responsible for more than half of all agricultural loss worldwide. Potexvirus is one of the two most important flexible filamentous plant viruses. Bamboo mosaic virus (BaMV), a single-stranded positive-sense RNA virus, is a member of the Potexvirus genus of Alphaflexiviridae. It can infect at least 12 species of bamboo, causing a huge economic impact on the bamboo industry in Taiwan. The study of BaMV did not start extensively until the completion of the full-length sequencing of genomic RNA of BaMV and generation of the BaMV infectious cDNA clone in the early 1990s. Since then, BaMV has been extensively studied at the molecular, cellular and ecological level, covering both basic and applied researches, by a group of researchers in Taiwan. In this eBook, the content comprises 6 reviews and 4 articles. Seven of them are involved in the infection of BaMV covering viral RNA replication, viral RNA trafficking, and the host factors. Two of them are related to the vector transmission and the ecology of BaMV. The last one is the application of using BaMV as a viral vector to produce vaccines in plants.

RNA interference (RNAi), a hallmark of all biological sciences of twenty-first century, is an evolutionarily conserved and double-stranded RNA-dependent eukaryotic cell defense process. Opportunity to utilize an organisms own gene and to systematically induce and trigger RNAi for any desired sequence made RNAi an efficient approach for functional genomics, providing a solution for conventional longstanding obstacles in life sciences. RNAi research and application have significantly advanced during past two decades. This book RNA interference provides an updated knowledge and progress on RNAi in various organisms, explaining basic principles, types, and property of inducers, structural modifications, delivery systems/methodologies, and various successful bench-to-field or clinic applications and disease therapies with some aspects of limitations, alternative tools, safety, and risk assessment.