Multidisciplinary Microfluidic and Nanofluidic Lab-on-a-Chip: Principles and Applications provides chemists, biophysicists, engineers, life scientists, biotechnologists, and pharmaceutical scientists with the principles behind the design, manufacture, and testing of life sciences microfluidic systems. This book serves as a reference for technologies and applications in multidisciplinary areas, with an emphasis on quickly developing or new emerging areas, including digital microfluidics, nanofluidics, papers-based microfluidics, and cell biology. The book offers practical guidance on how to design, analyze, fabricate, and test microfluidic devices and systems for a wide variety of applications including separations, disease detection, cellular analysis, DNA analysis, proteomics, and drug delivery. Calculations, solved problems, data tables, and design rules are provided to help researchers understand microfluidic basic theory and principles and apply this knowledge to their own unique designs. Recent advances in microfluidics and microsystems for life sciences are impacting chemistry, biophysics, molecular, cell biology, and medicine for applications that include DNA analysis, drug discovery, disease research, and biofluid and environmental monitoring. - Provides calculations, solved problems, data tables and design rules to help understand microfluidic basic theory and principles - Gives an applied understanding of the principles behind the design, manufacture, and testing of microfluidic systems - Emphasizes on quickly developing and emerging areas, including digital microfluidics, nanofluidics, papers-based microfluidics, and cell biology

Autonomous Robot-Aided Optical Manipulation for Biological Cells gives a systematically and almost self-contained description of the many facets of modeling, sensing, and control techniques or experimentally exploring emerging trends in optical manipulation of biological cell in micro/nanorobotics systems. To achieve biomedical applications, reliability design, modeling, and precision control are vitally important for the development of engineering systems. With the advances in modeling, sensing, and control techniques, it is opportunistic to exploit them for the benefit of reliability design, actuation, and precision control of micro/nanomanipulation systems to expanding the applications of robot at the micro and nano scales, especially in biomedical engineering. This book presents new techniques in reliability modeling and advanced control of robot-aided optical manipulation of biological cells systems. The book will be beneficial to the researchers within robotics, mechatronics, biomedical engineering, and automatic control society, including both academic and industrial parts. - Provides a series of latest results in, including but not limited to, design, sensing, actuation, modeling, and control of micro/nano manipulation system using optical tweezers - Gives recent advances of theory, technological aspects, and applications of advanced sensing, actuation, modeling and control methodologies in biomedical engineering applications - Offers simulation and experimental results in each chapter in order to reflect the biomedical engineering practice, yet demonstrate the main focus of the developed design, analysis and synthesis approaches

Publisher's Note: Products purchased from Third Party sellers are not guaranteed by the publisher for quality, authenticity, or access to any online entitlements included with the product. Cutting-Edge Optofluidics Theories, Techniques, and Practices Add novel functionalities to your optical design projects by incorporating state-of-the-art microfluidic technologies and tools. Co-written by industry experts, Optofluidics: Fundamentals, Devices, and Applications covers the latest functional integration of optical devices and microfluidics, as well as automation techniques. This authoritative guide explains how to fabricate optical lab-on-a-chip devices, synthesize photonic crystals, develop solid and liquid core waveguides, use fluidic self-assembly methods, and accomplish direct microfabrication in solutions. The book includes details on developing biological sensors and arrays, handling maskless lithography, designing high-Q cavities, and working with nanoscale plasmonics. Research outcomes from the DARPA-funded Center for Optofluidics Integration are also discussed. Discover how to: Work with optofluidic sources, lenses, filters, switches, and splitters Use dielectric waveguiding devices to input, move, and manipulate fluids Integrate colloidal crystals and fibers with microfluidic systems Develop bio-inspired fluidic lens systems and aspherical lenses Deploy miniaturized dye lasers, microscopes, biosensors, and resonators Analyze microfluidic systems using flow injection and fluorescent spectroscopy Build optofluidic direct fabrication platforms for innovative microstructures Accomplish optofluidic liquid actuation and particle manipulation

Optical trapping and manipulation by laser beams offers the unique possibility to handle single micrometer-sized particles such as living cells without any mechanical contact, damage or contamination. A second hot topic in biology is microfluidics, where the examination of biological samples in channel structures with widths below 100 µm reduces the used sample volume significantly. While the combination of both techniques results in attractive lab-on-a-chip structures for particle sorting and analysis, the commonly bulky trapping setup is contradictory to the miniaturized concept. Here, the use of vertical-cavity surface-emitting lasers (VCSELs) as light sources in optical trapping systems allows a strong reduction of the setup complexity owing to the small dimensions, low cost and high beam quality of these devices. This thesis gives a detailed study on optical manipulation systems based on vertically emitting laser diodes. A standard optical tweezers setup as well as a novel, miniaturized system, the so-called integrated optical trap are investigated. The latter aims for particle separation and sorting in microfluidics resulting in low-cost, portable modules. A classical optical tweezers system based on a high numerical aperture objective in combination with a VCSEL light source is investigated. Standard multi-mode as well as single-mode surface relief VCSELs are used as laser source. With both kinds of VCSELs, optical trapping of polystyrene particles of sizes ranging from 4 to 15µm is demonstrated with some milliwatts of optical power at the sample stage. A maximum trapping force of 4.4 pN for 15 µm particles is achieved with the multi-mode laser, proving the suitability of multi-mode lasers for optical manipulation despite their inferior beam profile. By using two-dimensional VCSEL arrays instead of solitary lasers, the system is extended to a multiple optical tweezers setup in a straightforward manner. To avoid any additional optics, densely packed VCSEL arrangements with a device spacing of less than 25 µm are used, where a novel fabrication process allows the seamless integration of the inverted surface relief technique for enhanced beam quality. By electrical switching between individual devices of the array, non-mechanical particle translation with velocities of up to 12 µm/s is achieved. With a tilted linear VCSEL array, an optical lattice is generated in the optical tweezers setup, and continuous deflection of particles is realized. By substituting the sample stage in the optical tweezers setup with a microfluidic chip fabricated from polydimethylsiloxane (PDMS), particle redirection at a channel junction is realized using a solitary VCSEL source as well as a tilted linear VCSEL array. For the latter, the particles are deflected when passing the optical lattice, thus, the position of the lasers is fixed and no moving parts are necessary, which further reduces the setup complexity. To achieve a drastic miniaturization of the trapping setup, namely the integrated optical trap, the laser source is placed directly underneath the sample chamber. A weakly focused laser beam is generated in the particle solution by integrating an additional microlens on the VCSEL output facet. To determine appropriate lens geometries, the beam propagation inside the integrated trap structure is calculated and the thermal reflow process for lens fabrication is studied in detail concerning lens diameter, reflow temperature and substrate material. By combining the microlens with the inverted relief technique, the quality of the focused beam is strongly improved with respect to divergence, transverse beam profile and beam diameter, where a minimum of 7 µm is measured at the focal point. With first solitary integrated optical traps, deflection, levitation and transverse trapping of 10 µm polystyrene particles is demonstrated for optical powers of 5mW. In a next step, integrated optical trap arrays are realized based on closely spaced twodimensional arrangements of lensed relief VCSELs. To transfer the continuous deflection scheme demonstrated in the classical tweezers setup to the integrated trap, linear arrays of parallel working VCSELs are investigated. To support the design of the multiple integrated trap structure, a simulation of the optical deflection process is performed. Here, a dependence on the geometric and material properties of the particles is predicted, so applications in microfluidic particle sorting are intended. Compact and portable modules are obtained by integrating the laser chip with the microfluidic chip using flip-chip bonding. Although the finished modules show strong heating of the VCSEL chip resulting in a significant reduction of the device performance, simultaneous trapping as well as continuous particle deflection was successfully demonstrated with a total optical power of just 5mW. The results presented in this work demonstrate the potential of VCSELs as laser sources for optical trapping and microparticle manipulation. In conventional optical tweezers setups, the use of VCSELs reduces the setup complexity significantly, while first prototypes of ultra-compact integrated optical traps based on VCSELs confirm the feasibility of portable and inexpensive microfluidic sorting systems.