The completely revised second edition of this user-friendly and application-oriented overview of one-step biotransformations of industrial importance. Based on extensive literature and patent research, this book is unique in arranging each process in a systematic way to allow for easy comparison. All the chapters have been rewritten, with all the processes updated and more than 30 new processes added. Each set of data is accompanied by key literature citations, supported by flow sheets where available, reduced to their significant elements. In addition, an extensive index classified by substrates, products, enzymes, and companies provides direct access to each process, organized according to enzyme class. Biotechnologists, biochemists, microbiologists, process engineers and those working in the chemical and biotechnological industries will find here all the significant parameters characterizing both the biotransformation and the process.

Industrial Biotransformations - a user-friendly and application-oriented up-to-date overview of one-step biotransformations of industrial importance. The data conferring each process is arranged in a convenient format to survey so that the processes can easily be compared. Each set of data is accompanied by key literature citations. As far as flow sheets of the processes are available, these are given reduced to their significant elements. An extensive index classified by substrates, products, enzymes, and companies provides direct access to each process organized in the order of enzyme classes. The reader will find all significant parameters characterizing the biotransformation itself and the process.

This volume provides a clear understanding of how microbes, following their degradative processes, contribute maximally to the benefit of mankind through biotransformations of waste materials as well as a wide variety of health-risk compounds.The book contains twenty four chapters contributed by leading scientists from different parts of the world, covering various aspects of bioremediation of xenobiotics such as toxic, carcinogenic, teratogenic, and mutagenic compounds, which include halogenated aromatics, derivatives of heavy metals, microbial toxins, tannins, dyes, sulfur compounds of coal and petroleum and pesticides. The bioremediation of agricultural residue, industrial as well as municipal wastes, fuel oils, lubricants, natural rubber products, and other synthetic polymers, which pollute the environment substantially, also constitutes an important component of the book. All biotechnological aspects of microbial transformations pertaining to biodegradation/bioremediation of hazardous wastes, ranging from screening methods for microbes with degradative potential, processes of degradation, strain improvement for enhanced biodegradation and elimination of xenobiotics of health and environmental concern have been dealt with. The book intends to widen the scope of Applied Microbiology and Biotechnology in general and biotransformations in particular. It will provide an opportunity for scientists in the areas of biochemistry, food industry, environmental science and engineering and their implications in technologically feasible, environment friendly and economically viable bioremediation options. Also, it forms an interface between agro-industrial establishments and the academic world and will generate new thought provoking ideas for scientists of future generations for the safeguard of both human and animal health as well as the environment.

From the laboratory to full-scale commercial production, this reference provides a clear and in-depth analysis of bioreactor design and operation and encompasses critical aspects of the biocatalytic manufacturing process. It clarifies principles in reaction and biochemical engineering, synthetic and biotransformation chemistry, and biocell and enzy

A comprehensive and consolidated account of how microorganisms can play a significant role in degrading and detoxifying toxic, carcinogenic, mutagenic, and teratogenic compounds is detailed in this book. Moreover, the volume deals with all aspects of microbial degradation, ranging from screening methods for the degradative microorganisms, processes of degradation, strain improvement for enhanced biodegradation, and elimination of undesirable compounds to improving health and environmental protection strategies.The book will provide an opportunity for scientists in the areas of microbiology, biochemistry, engineering, food science, biotechnology, and environmental science to obtain a clear understanding of microbial biotransformations of xenobiotics, as well as an interface between industry and the academic world. The first book of its kind, it will open new vistas of research in the field of Applied Microbiology and Biotechnology in general, and Biotransformations in particular.

Biocatalysts are increasingly used by chemists engaged in fine chemical synthesis within both industry and academia. Today, there exists a huge choice of high-tech enzymes and whole cell biocatalysts, which add enormously to the repertoire of synthetic possibilities. Practical Methods for Biocatalysis and Biotransformations 2 is a "how-to" guide that focuses on the practical applications of enzymes and strains of microorganisms that are readily obtained or derived from culture collections. The sources of starting materials and reagents, hints, tips and safety advice (where appropriate) are given to ensure, as far as possible, that the procedures are reproducible. Comparisons to alternative methodology are given and relevant references to the primary literature are cited. This second volume – which can be used on its own or in combination with the first volume - concentrates on new applications and new enzyme families reported since the first volume. Contents include: introduction to recent developments and future needs in biocatalysts and synthetic biology in industry reductive amination enoate reductases for reduction of electron deficient alkenes industrial carbonyl reduction regio- and stereo- selective hydroxylation oxidation of alcohols selective oxidation industrial hydrolases and related enzymes transferases for alkylation, glycosylation and phosphorylation C-C bond formation and decarboxylation halogenation/dehalogenation/heteroatom oxidation tandem and sequential multi-enzymatic syntheses Practical Methods for Biocatalysis and Biotransformations 2 is an essential collection of biocatalytic methods for chemical synthesis which will find a place on the bookshelves of synthetic organic chemists, pharmaceutical chemists, and process R&D chemists in industry and academia.

Leading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes. These readily reproducible methods can be used to improve enzyme function by directed evolution, to covalently immobilize enzymes, to microencapsulate enzymes and cells, and to manufacture enzymes for human health, nutrition, and environmental protection. Overview chapters on microorganisms as a source of metabolic and enzymatic diversity, and on the fast-moving field of enzyme biosensors are presented. Microbial Enzymes and Biotransformations offers laboratory and industrial scientists a wealth of proven enzymatic protocols that show clearly how to go from laboratory results to successful industrial applications.