The book presents a clear and comprehensive review of the current status of the holographic microscopy with discussion of the positive and negative features of classical and holographic methods for solving the problem of three-dimesional (3D) imaging of phase microscopic objects. Classical and holographic methods of phase, interference and polarization contrast are discussed. Combination of the developed holographic methods with the methods of digital image processing allowed creating the digital holographic interference microscope (DHIM). The first 3D images of native phase microscopic objects such as blood cells were obtained using the DHIM. The results of DHIM application for study of blood erythrocytes, thin films, micro-crystals are presented.

This book presents a clear and comprehensive review of the current status of three-dimensional (3D) digital holographic imaging of phase microscopic objects, with insightful discussions on the positive and negative features of classical, electronic and holographic microscopy. The technical details and results of the restoration of the 3D shapes of red blood cells, bacteria, yeasts, thin films and other micro-objects are presented. The physical background of the method was substantiated by the authors in 1989 and in 1998, the very first digital holographic interference microscope was developed. Clear evidence of the pathological flattened shape of the erythrocytes relevant to different pathologies is given by detailed measurements on the 3D images. Based on the model of the erythrocyte as a liquid-filled, charged, and viscoelastic shell reinforced by skeleton, numerical computations of the equilibrium shapes of human erythrocytes are made, and the results are analyzed and compared to the 3D visualization data

Building up from the basic principles of optics, this straightforward introduction to digital holography, aimed at graduate students, engineers and researchers, describes modern techniques and applications, plus all the necessary underlying theory. Supporting Matlab code is available for download online, and homework problems are accompanied by an instructor solution manual.

This book deals with the latest achievements in the field of optical coherent microscopy. While many other books exist on microscopy and imaging, this book provides a unique resource dedicated solely to this subject. Similarly, many books describe applications of holography, interferometry and speckle to metrology but do not focus on their use for microscopy. The coherent light microscopy reference provided here does not focus on the experimental mechanics of such techniques but instead is meant to provide a users manual to illustrate the strengths and capabilities of developing techniques. The areas of application of this technique are in biomedicine, medicine, life sciences, nanotechnology and materials sciences.

Holography - Basic Principles and Contemporary Applications is a collection of fifteen chapters, describing the basic principles of holography and some recent innovative developments in the field. The book is divided into three sections. The first, Understanding Holography, presents the principles of hologram recording illustrated with practical examples. A comprehensive review of diffraction in volume gratings and holograms is also presented. The second section, Contemporary Holographic Applications, is concerned with advanced applications of holography including sensors, holographic gratings, white-light viewable holographic stereograms. The third section of the book Digital Holography is devoted to digital hologram coding and digital holographic microscopy.

The measurement and characterisation of surface topography is crucial to modern manufacturing industry. The control of areal surface structure allows a manufacturer to radically alter the functionality of a part. Examples include structuring to effect fluidics, optics, tribology, aerodynamics and biology. To control such manufacturing methods requires measurement strategies. There is now a large range of new optical techniques on the market, or being developed in academia, that can measure areal surface topography. Each method has its strong points and limitations. The book starts with introductory chapters on optical instruments, their common language, generic features and limitations, and their calibration. Each type of modern optical instrument is described (in a common format) by an expert in the field. The book is intended for both industrial and academic scientists and engineers, and will be useful for undergraduate and postgraduate studies.

Digital holography is an emerging field of new paradigm in general imaging applications. The book presents an introduction to the theoretical and numerical principles and reviews the research and development activities in digital holography, with emphasis on the microscopy techniques and applications. Topics covered include the general theory of diffraction and holography formations, and practical instrumentation and experimentation of digital holography. Various numerical techniques are described that give rise to the unique and versatile capabilities of digital holography. Representative special techniques and applications of digital holography are discussed. The book is intended for researchers interested in developing new techniques and exploring new applications of digital holography.