Volumes 7-77, 80-83 include 13th-83rd, 86th-89th annual report of the American Baptist missionary union.

Volumes 7-77, 80-83 include 13th-83rd, 86th-89th annual report of the American Baptist missionary union.

This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity labeling methods, the volume continues with BioID, proximity labeling methods that describe the proximity ligation assay to detect RNA-DNA interactions, UV cross-linking to demonstrate RNA-protein interactions, and how chemical and enzymatic reactivities can be improved upon DNA-DNA and protein-protein interactions, as well as “proximity-induced self-labeling,” where the radius of labeling is zero. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Proximity Labeling: Methods and Protocols serves as an ideal guide for researchers exploring the crucial roles that proximity-driven reactions play in biological systems.

Never were energy use and security of energy supply as high on the international political agendas as they are now. There seems to be a consensus that energy savings and sustainable energy production must have a high priority. Because the energy use in the residential and non-residential sectors account for a large part of the total energy use, new programs are being developed in order to limit the consumption of energy in these sectors and a lot of attention is put into increasing the energy efficiency of the existing building stock. Towards a Sustainable Northern European Housing Stock is based on a search and inventory of data on the existing Northern-European building stock and related policy developments. In this book, the authors try to give a realistic image of what is really known about the current quality of the building stock, the type of renovation activities that are undertaken and the policies being currently implemented. Recommendations are also made on how to improve the present situation.