Histochemistry and cytochemistry are important fields for studying the inner workings of cells and tissues of the body. While visualization techniques have been in use for many years, new methods of detection developed recently help researchers and practitioners better understand cell activity. Histochemical and Cytochemical Methods of Visualization describes the essential techniques that can be used for histochemical investigations in both light and transmission electron microscopy. The book begins by discussing techniques in light microscopy. It reviews classical methods of visualization, histochemical and histoenzymatic methods, and methods used to visualize cell proliferation and apoptosis. Next, the book examines the cytochemical methods used in electron microscopy with traditional techniques, as well as more specialized methods. The final section provides an overview of image analysis and describes how image processing methods can be used to extract vital information. A 16-page insert supplies color illustrations to enhance the text. Techniques will continue to adapt to the latest technological innovations, allowing more and more precise quantification of images. These developments are essential to the biological as well as the medical sciences. This manual is a critical resource for novice and experienced researchers, technicians, and students who need to visualize what happens in the cell, the molecules expressed, the main enzymatic activities, and the repercussions of the molecular activities upon the structure of the cells in the body.

Histochemistry and cytochemistry are important fields for studying the inner workings of cells and tissues of the body. While visualization techniques have been in use for many years, new methods of detection developed recently help researchers and practitioners better understand cell activity. Histochemical and Cytochemical Methods of Visualizatio

The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.

This fifth edition of Histological and Histochemical Methods continues to provide a clear and consistent introduction to the techniques, description and analysis of the chemical and physical principles of fixation, tissue processing, staining, enzyme location, immunohistochemistry and other key procedures. The overall structure of the book remains unchanged, but the content has been heavily revised to update the techniques used in line with recent technological advances. Additionally, there are new sections on: Artefacts and troubleshooting Methods for microorganisms and fungi in sections Methods for various pigments and mineral deposits in tissues Methods for skeletal elements (bone, cartilage) in whole-mounts Histological and Histochemical Methods 5e is essential reading for students, lecturers, researchers and professionals using histological and histochemical techniques. From reviews: "Histological and Histochemical Methods is a tour de force wholly suited to the modern age of histology and Professor Kiernan has triumphed again. To cover so much ground clearly and concisely while including the justification of the underlying chemistry makes this book unique. There should not be a histology laboratory or an undergraduate library that does not own a copy." Biotechnic & Histochemistry 2016, 91(2): 145. "This book should be present on the bookshelves of every research or analysis laboratory where histology and histochemistry are routinely used, as an essential reference source of basic and practical information for scientists and technicians." European Journal of Histochemistry, 2016, vol. 60.

Approx.3876 pages Approx.3876 pages

The biological sciences cover a broad array of literature types, from younger fields like molecular biology with its reliance on recent journal articles, genomic databases, and protocol manuals to classic fields such as taxonomy with its scattered literature found in monographs and journals from the past three centuries. Using the Biological Litera

New interest in light microscopy of the last few years has not been backed up by adequate general literature. This book intends to fill the gap between specialized texts on detailed topics and general introductory booklets, mostly dealing with the use of the conventional light microscope only. In this short textbook both new developments in microscopy and basic facts of image formation will be treated, including often neglected topics such as axial resolving power, lens construction, photomicrography and correct use of phase-en interference contrast systems. Theoretical background will be dealt with as far as necessary for a well-considered application of these techniques enabling a deliberate choice for the approach of a certain problem. Over 150 illustrations (photomicrographs and diagrams) complete the information on microscopy of the nineties in the biomedical field, intended for scientists, doctors, technicians and research students. Many drawings have been contributed by the illustrator R. Kreuger; the photographic work has been executed by J. Peeterse. Secretarial assistance in preparing the manuscript was given by Ms T. M. S. Pierik. Dr M. J. Pearson has corrected the English of the final text.