This comprehensive account of the human herpesviruses provides an encyclopedic overview of their basic virology and clinical manifestations. This group of viruses includes human simplex type 1 and 2, Epstein–Barr virus, Kaposi's Sarcoma-associated herpesvirus, cytomegalovirus, HHV6A, 6B and 7, and varicella-zoster virus. The viral diseases and cancers they cause are significant and often recurrent. Their prevalence in the developed world accounts for a major burden of disease, and as a result there is a great deal of research into the pathophysiology of infection and immunobiology. Another important area covered within this volume concerns antiviral therapy and the development of vaccines. All these aspects are covered in depth, both scientifically and in terms of clinical guidelines for patient care. The text is illustrated generously throughout and is fully referenced to the latest research and developments.

This second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR/Cas9 engineering, and the use of BioID to identify protein-protein interactions. Chapters cover topics such as the biology, life cycle, and current state of antiviral and vaccine development for HSV-1; protocols on growing viruses in cell culture and manipulating viral DNA; design and application of HSV-1 vectors for cancer- and gene-therapy; and structural analyses, microscopy, proteomics, and testing of antivirals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Herpes Simplex Virus: Methods and Protocols, Second Edition is a valuable resource for immunologists, and molecular and cell biologists. This book will also be useful for researchers who wish to initiate molecular and/or cellular-based approaches to study HSV.

This book is designed to present a comprehensive and state-of the-art update that covers the pathophysiology, epidemiology, and clinical presentation of the most frequently encountered STIs in adolescence and young adulthood. The introductory sections discuss more general themes including approaches to obtaining a sexual history and exam, concerns of sexual minority youth, ethical and legal considerations, and health disparities in STIs in this population. Subsequent chapters are organized by pathogen such as herpes simplex virus, and human immunodeficiency virus, or clinical syndrome including pelvic inflammatory disease, and vaginitis. Each chapter begins with a case study to illustrate key characteristics of the disease process in question and includes rich illustrations, resources, and guidelines. Written by experts in the field, the text includes a review of epidemiology, pathophysiology, treatment, prevention, and adolescent-specific considerations that is vital to working with this important population. With its transdisciplinary perspective, Sexually Transmitted Infections in Adolescence and Young Adulthood is a unique text that is valuable to infectious disease specialists, adolescent medicine specialists, gynecologists, primary care physicians, advanced practice providers, medical administrative staff, school nurses, sexual health educators, social workers, and public health officials.

Vaccines have made it possible to eradicate the scourge of smallpox, promise the same for polio, and have profoundly reduced the threat posed by other diseases such as whooping cough, measles, and meningitis. What is next? There are many pathogens, autoimmune diseases, and cancers that may be promising targets for vaccine research and development. This volume provides an analytic framework and quantitative model for evaluating disease conditions that can be applied by those setting priorities for vaccine development over the coming decades. The committee describes an approach for comparing potential new vaccines based on their impact on morbidity and mortality and on the costs of both health care and vaccine development. The book examines: Lessons to be learned from the polio experience. Scientific advances that set the stage for new vaccines. Factors that affect how vaccines are used in the population. Value judgments and ethical questions raised by comparison of health needs and benefits. The committee provides a way to compare different forms of illness and set vaccine priorities without assigning a monetary value to lives. Their recommendations will be important to anyone involved in science policy and public health planning: policymakers, regulators, health care providers, vaccine manufacturers, and researchers.

​​​ Inpatient Dermatology is a concise and portable resource that synthesizes the most essential material to help physicians with recognition, differential diagnosis, work-up, and treatment of dermatologic issues in the hospitalized patient. Complete with hundreds of clinical and pathologic images, this volume is both an inpatient dermatology atlas and a practical guide to day-one, initial work-up, and management plan for common and rare skin diseases that occur in the inpatient setting. Each chapter is a bulleted, easy-to-read reference that focuses on one specific inpatient dermatologic condition, with carefully curated clinical photographs and corresponding histopathologic images to aid readers in developing clinical-pathologic correlation for the dermatologic diseases encountered in the hospital. Before each subsection the editors share diagnostic pearls, explaining their approach to these challenging conditions. This book is structured to be useful to physicians, residents, and medical students. It spans dermatology, emergency medicine, internal medicine, infectious disease, and rheumatology. Inpatient Dermatology is the go-to guide for hospital-based skin diseases, making even the most complex inpatient dermatologic issues approachable and understandable for any clinician.

Advances in genomic and proteomic profiling of disease have transformed the field of molecular diagnostics, thus leading the way for a major revolution in clinical practice. While the range of tests for disease detection and staging is rapidly expanding, many physicians lack the knowledge required to determine which tests to order and how to interpret results. Molecular Diagnostics provides a complete guide to the use and interpretation of molecular testing in the clinical arena. No other available resource offers this emphasis, comprehensive scope, and practical utility in the clinical setting. - Serves as the definitivereference for molecular pathologists worldwide - Covers a variety of molecular techniques including next generation sequencing, tumor somatic cell genotyping, infectious and genetic disease tecting, and pharmacogenetics - Discusses in the detail issues concerning quality assurance, regulation, ethics, and future directions for the science

This is a comprehensive, practical, densely illustrated diagnostic and therapeutic guide for all geriatric dermatology providers. The book comprises 50 chapters and over 600 color photographs on topics ranging from common conditions such as basal cell carcinoma, rosacea, and seborrheic dermatitis to unusual conditions such as angiosarcoma, dermatofibrosarcoma protuberans, and porphyria cutanea tarda. Sections include: - Inflammatory conditions (including contact dermatitis, alopecia, erythema multiforme, pemphigus, bullous pemphigoid, porphyria, pruritus, psoriasis, rosacea, seborrhea, urticaria, xerosis, and more) - Infections (fungus, herpes simplex and zoster, scabies, lice, and warts) - Skin signs in systemic disease (skin tags, cutaneous metastases, xanthomas) - Regional dermatoses (intertrigo, leg ulcers, pressure sores) - Benign tumors (chondrodermatitis, cysts, ganglion, fibrous papule, seborrheic keratoses, lentigines, and benign vascular lesions) - Pre-malignant and malignant tumors (actinic keratoses, angiosarcoma, basal cell carcinoma, dermatofibroma and dermatofibrosarcoma protuberans, intraepidermal neoplasia, Kaposi's sarcoma, keratoacanthoma, lentigo maligna, cutaneous lymphoma, Mycosis fiungoides, melanoma, nevi and moles, and squamous cell carcinoma)