"With a history that likely dates back to the dawn of human civilization more than 10,000 years ago, and a record that includes the domestication and selective breeding of plants and animals, the harnessing of fermentation process for bread, cheese and brewage production, and the development of vaccines against infectious diseases, biotechnology has acquired a molecular focus during the 20th century, particularly following the resolution of DNA double helix in 1953, and the publication of DNA cloning protocol in 1973, and transformed our concepts and practices in disease diagnosis, treatment and prevention, pharmaceutical and industrial manufacturing, animal and plant industry, and food processing. While molecular biotechnology offers unlimited opportunities for improving human health and wellbeing, animal welfare, agricultural innovation and environmental conservation, a dearth of high quality books that have the clarity of laboratory manuals without distractive procedural details and the thoroughness of well-conversed textbooks appears to damp the enthusiasm of aspiring students. In attempt to fill this glaring gap, Handbook of Molecular Biotechnology includes four sections, with the first three presenting in-depth coverage on DNA, RNA and protein technologies, and the fourth highlighting their utility in biotechnology. Recognizing the importance of logical reasoning and experimental verification over direct observation and simple description in biotechnological research and development, the introductory provides pertinent discussions on key strategies (i.e., be first, be better, and be different), effective thinking (lateral, parallel, causal, reverse and random), and experimental execution, which have proven invaluable in helping advance research projects, evaluate and prepare research reports, and enhance other scientific endeavors"--

The only textbook of its kind on the market, Molecular Biotechnology provides a holistic, comprehensive view of molecular biotechnology that makes it ideally suited for undergraduate majors in molecular biotechnology and biomedical sciences. Beginning with the background of this rapidly expanding field, Molecular Biotechnology covers major discoveries, regulation of the biotechnology industry, and significant innovations. A strong emphasis on careers in molecular biotechnology, profiles of major projects and researchers, and expansive discussions of bioethical concerns and current research, all come together to make this text an engaging and highly relevant resource for biotechnology students.

The enormous complexity of biological systems at the molecular level must be answered with powerful computational methods. Computational biology is a young field, but has seen rapid growth and advancement over the past few decades. Surveying the progress made in this multidisciplinary field, the Handbook of Computational Molecular Biology of

The branch of biology that is concerned with the study of the molecular basis of biological activities within and between the cells is known as molecular biology. It encompasses molecular synthesis, mechanisms, modifications and interactions. It is closely related to the fields of biochemistry, genetics, computational biology and bioinformatics. Molecular genetics is one of the most prominent sub-fields of this area. Molecular cloning, gel electrophoresis, polymerase chain reaction, macromolecule blotting and probing, allele-specific oligonucleotide, microarrays, etc. are some of the techniques used within this discipline. This book brings forth some of the most innovative concepts and elucidates the unexplored aspects of molecular biology. The various studies that are constantly contributing towards advancing technologies and evolution of this field are examined in detail. Those in search of information to further their knowledge will be greatly assisted by this book.

Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sa

The second edition explains the principles of recombinant DNA technology as well as other important techniques such as DNA sequencing, the polymerase chain reaction, and the production of monclonal antibodies.

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions