Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology

Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sapiens), model crucifer (Arabidopsis thaliana), and rice (Oryza sativa). The invention of DNA microarray technology and advances in bioinformatics have generated vast amounts of genomic data. Reflecting these revolutionary advances Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition documents conventional and modern approaches to tackle scientific research in the post-genomics era. Maintaining the step-by-step format that popularized the first edition, each chapter provides the principles behind the featured method, a detailed description of each protocol, applications of the protocol to different systems, and references for further study. Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes: New protocols in all chapters, including alternative protocols In vitro transcription methods Analysis of DNA sequences New bioseparation techniques New chapters covering: mRNA differential display Inhibition of gene expression In situ hybridization (Localization of gene expression) Combinatorial techniques Computational data mining methods applied to combinatorial chemistry libraries With this book at hand, researchers, teachers, and students can understand and utilize the major techniques and methods currently employed in cellular and molecular biology.

Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method. This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies. The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!

Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method. This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies. The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!

Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.

In considering ways that physics has helped advance biology and medicine, what typically comes to mind are the various tools used by researchers and clinicians. We think of the optics put to work in microscopes, endoscopes, and lasers; the advanced diagnostics permitted through magnetic, x-ray, and ultrasound imaging; and even the nanotools, that a

This book provides an entry point into Systems Biology for researchers in genetics, molecular biology, cell biology, microbiology and biomedical science to understand the key concepts to expanding their work. Chapters organized around broader themes of Organelles and Organisms, Systems Properties of Biological Processes, Cellular Networks, and Systems Biology and Disease discuss the development of concepts, the current applications, and the future prospects. Emphasis is placed on concepts and insights into the multi-disciplinary nature of the field as well as the importance of systems biology in human biological research. Technology, being an extremely important aspect of scientific progress overall, and in the creation of new fields in particular, is discussed in 'boxes' within each chapter to relate to appropriate topics. - 2013 Honorable Mention for Single Volume Reference in Science from the Association of American Publishers' PROSE Awards - Emphasizes the interdisciplinary nature of systems biology with contributions from leaders in a variety of disciplines - Includes the latest research developments in human and animal models to assist with translational research - Presents biological and computational aspects of the science side-by-side to facilitate collaboration between computational and biological researchers