A diverse team of researchers, technologists, and engineers describe, in simple and practical language, the major current and evolving technologies for improving the biocatalytic capabilities of mammalian, microbial, and plant cells. The authors present state-of-the-art techniques, proven methods, and strategies for industrial screening, cultivation, and scale-up of these cells, and describe their biotech and industrial uses. Special emphasis is given to the solving critical issues encountered during the discovery of new drugs, process development, and the manufacture of new and existing compounds. Other topics include recombinant protein expression, bioinformatics, high throughput screening, analytical tools in biotechnology, DNA shuffling, and genomics discovery.

This book is the culmination of three decades of accumulated experience in teaching biotechnology professionals. It distills the fundamental principles and essential knowledge of cell culture processes from across many different disciplines and presents them in a series of easy-to-follow, comprehensive chapters. Practicality, including technological advances and best practices, is emphasized. This second edition consists of major updates to all relevant topics contained within this work. The previous edition has been successfully used in training courses on cell culture bioprocessing over the past seven years. The format of the book is well-suited to fast-paced learning, such as is found in the intensive short course, since the key take-home messages are prominently highlighted in panels. The book is also well-suited to act as a reference guide for experienced industrial practitioners of mammalian cell cultivation for the production of biologics.

The editors have enlisted a broad range of experts, including microbial ecologists, physiologists, geneticists, biochemists, molecular biologists, and biochemical engineers, who offer practical experience not found in texts and journals. This comprehensive perspective makes MIMB a valuable "how to" resource, the structure of which resembles the sequence of operation involved in the development of a commercial biological process and product.

Handbook of Microalgal Culture is truly a landmarkpublication, drawing on some 50 years of worldwide experience inmicroalgal mass culture. This important book comprisescomprehensive reviews of the current available information onmicroalgal culture, written by 40 contributing authors from aroundthe globe. The book is divided into four parts, with Part I detailingbiological and environmental aspects of microalgae with referenceto microalgal biotechnology and Part II looking in depth at majortheories and techniques of mass cultivation. Part III compriseschapters on the economic applications of microalgae, includingcoverage of industrial production, the use of microalgae in humanand animal nutrition and in aquaculture, in nitrogen fixation,hydrogen and methane production, and in bioremediation of pollutedwater. Finally, Part IV looks at new frontiers and includeschapters on genetic engineering, microalgae as platforms forrecombinant proteins, bioactive chemicals, heterotrophicproduction, microalgae as gene-delivery systems for expressingmosquitocidal toxins and the enhancement of marine productivity forclimate stabilization and food security. Handbook of Microalgal Culture is an essential purchasefor all phycologists and also those researching aquatic systems,aquaculture and plant sciences. There is also much of great use toresearchers and those involved in product formulation withinpharmaceutical, nutrition and food companies. Libraries in alluniversities and research establishments teaching and researchingin chemistry, biological and pharmaceutical sciences, food sciencesand nutrition, and aquaculture will need copies of this book ontheir shelves. Amos Richmond is at the Blaustein Institute for DesertResearch, Ben-Gurion University of the Negev, Israel.

This is a well-rounded handbook of fermentation and biochemical engineering presenting techniques for the commercial production of chemicals and pharmaceuticals via fermentation. Emphasis is given to unit operations fermentation, separation, purification, and recovery. Principles, process design, and equipment are detailed. Environment aspects are covered. The practical aspects of development, design, and operation are stressed. Theory is included to provide the necessary insight for a particular operation. Problems addressed are the collection of pilot data, choice of scale-up parameters, selection of the right piece of equipment, pinpointing of likely trouble spots, and methods of troubleshooting. The text, written from a practical and operating viewpoint, will assist development, design, engineering and production personnel in the fermentation industry. Contributors were selected based on their industrial background and orientation. The book is illustrated with numerous figures, photographs and schematic diagrams.

The submersed cultivation of organisms in sterile containments or fermenters has become the standard manufacturing procedure, and will remain the gold standard for some time to come. This book thus addresses submersed cell culture and fermentation and its importance for the manufacturing industry. It goes beyond expression systems and integrally investigates all those factors relevant for manufacturing using suspension cultures. In so doing, the contributions cover all industrial cultivation methods in a comprehensive and comparative manner, with most of the authors coming from the industry itself. Depending on the maturity of the technology, the chapters address in turn the expression system, basic process design, key factors affecting process economics, plant and bioreactor design, and regulatory aspects.

Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.