Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science.

Now in its third edition, this bestselling work continues to offer state-of-the-art information on the development and employment of capillary electrophoresis. With special emphasis on microseparations and microfluidics, it features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Enhanced by contributions from leading international experts, the Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition remains a seminal reference for the chemistry, biology, and engineering fields.

Over the last decade, high performance Capillary electrophoresis (HPCE) has emerged as a powerful and versatile separation technique that promises to rival high performance liquid chromatography when applied to the separation of both charged and neutral species. The high speed and high separation efficiency which can be attained using any of the various modes of HPCE has resulted in the increased use of the technique in a range of analytical environments. The procedures are, however, still in the early stages of development and several barriers remain to their adoption as the technique of choice for a range of analytical problems. One such barrier is the selection and optimization of the conditions required to achieve reproducible separations of analytes and it is in this area that this new book seeks to give assistance. The book is written by an international team of authors, drawn from both academic and industrial users, and the manufacturers of instruments. At its heart are a number of tables, divided into specific application areas. These give details of published separations of a wide range of archetypal analytes, the successful separation conditions and the matrix in which they were presented. These tables are based on separations reported since 1992 and are fully referenced to the original literature. The tables are supported by discussions of the problems that a particular area presents and the strategies and solutions adopted to overcome them. The general areas covered are biochemistry, pharmaceutical science, bioscience, ion analysis, food analysis and environmental science.

Capillary zone electrophoresis : basic concepts -- Capillary zone electrophoresis : methods development -- Capillary zone electrophoresis : secondary equilibrium, micelles, cyclodextrins, and related reagents -- Capillary isoelectric focusing -- Size separations in capillary gels and polymer networks -- Capillary electrochromatography -- Injection -- Detection -- Putting it all together.

Although capillary electrophoresis (CE) technology has evolved quickly from the research laboratory into practical application in numerous fields, many scientists still debate its merits. While the body of international CE literature continues to expand dramatically, experts still question whether it has provided the speed, resolving power, peak capacity, sensitivity, robustness, and cost-reduction promised by its pioneers. Responding to these criticisms, this third edition brings together cutting-edge researchers to demonstrate the utility of CE across a broad spectrum of disciplines including— Forensic science Medical diagnostics Pharmaceutical science Genetic analysis Biotechnology Fluid mechanics Environmental science Biomedical research Nanotechnology Proteomics Detailed Analysis of New Methodologies and Applications Eagerly awaited by researchers and technicians who transformed the first two editions into bestsellers, this latest volume once again delivers. Emphasizing microseparations and microfluidics, the Handbook of Capillary and Microchip Electrophoresis, Third Edition features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Aided by contributions from leading international experts, this text remains a seminal reference for numerous chemistry, biology, and engineering fields.

In the 1980s, capillary electrophoresis (CE) joined high-performance liquid chromatography (HPLC) as the most powerful separation technique available to analytical chemists and biochemists. Published research using CE grew from 48 papers in the year of commercial introduction (1988) to 1200 in 1997. While only a dozen major pharmaceutical and biotech companies have reduced CE to routine practice, the applications market is showing real or potential growth in key areas, particularly in the DNA marketplace for genomic mapping and forensic identification. For drug development involving small molecules (including chiral separations), one CE instrument can replace 10 liquid chromatographs in terms of speed of analysis. CE also uses aqueous rather than organic solvents and is thus environmentally friendlier than HPLC. The second edition of Practical Capillary Electrophoresis has been extensively reorganized and rewritten to reflect modern usage in the field, with an emphasis on commercially available apparatus and reagents. This authoritative and very comprehensible treatment builds on the author's extensive experience as an instructor of short courses for the American Chemical Society and for industry. - Illustrated with detailed diagrams of electrophoretic phenomena - Offers step-by-step methods development schemes - Presents techniques for developing quantitative, robust, and precise methods - Includes an extensive troubleshooting guide - Updates and greatly expands on the first edition-more than 50% of the text is new - Written by an internationally recognized scientist who is an instructor for American Chemical Society short courses on HPCE

Although capillary electrophoresis (CE) technology has evolved quickly from the research laboratory into practical application in numerous fields, many scientists still debate its merits. While the body of international CE literature continues to expand dramatically, experts still question whether it has provided the speed, resolving power, peak capacity, sensitivity, robustness, and cost-reduction promised by its pioneers. Responding to these criticisms, this third edition brings together cutting-edge researchers to demonstrate the utility of CE across a broad spectrum of disciplines including-- Forensic science Medical diagnostics Pharmaceutical science Genetic analysis Biotechnology Fluid mechanics Environmental science Biomedical research Nanotechnology Proteomics Detailed Analysis of New Methodologies and Applications Eagerly awaited by researchers and technicians who transformed the first two editions into bestsellers, this latest volume once again delivers. Emphasizing microseparations and microfluidics, the Handbook of Capillary and Microchip Electrophoresis, Third Edition features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Aided by contributions from leading international experts, this text remains a seminal reference for numerous chemistry, biology, and engineering fields.