Published on behalf of the Biological Stain Commission For 75 years Conn's Biological Stains has been a standard reference for all those who used dyes and colorants in the biological and medical sciences. This long awaited tenth edition appears 25 years after R.D. Lillie's ninth and has been completely rewritten to reflect the increase in range of uses. Although the staining of microscopical preparations continues to expand the uses of dyes and fluorochromes now extend far beyond this traditional application. This book provides the first critical overview of the whole range of low molecular weight fluorescent probes, outside the catalogue literature. The first ten chapters are essays, by leading experts, on the important aspects of colorants and their uses. Most of the remainder of the book consists of descriptions by Dr Horobin of the properties and recent applications of hundreds of individual compounds, in about twenty chemical classes. The last chapter reviews the procedures employed at the Biological Stain Commission's laboratory to assay and test dyes and certify them as suitable for their intended applications.

A complete, up-to-date resource of information on more than 200 dyes and stains Handbook of Biological Dyes and Stains is the most comprehensive volume available on the subject, covering all the available dyes and stains known to date in the literature for use in biology and medicine. Top dye expert Dr. Ram Sabnis organizes the compounds alphabetically by the most commonly used chemical name. He presents an easy-to-use reference complete with novel ideas for breakthrough research in medical, biological, chemical, and related fields. This is the first book to give the CAS registry number, chemical structure, Chemical Abstracts index name, all other chemical names, Merck Index number, chemical/dye class, molecular formula, molecular weight, physical form, solubility, melting point, boiling point, pH range, color change at pH, pKa, absorption, and emission maxima of dyes and stains, as well as to provide access to synthesis procedures (lab scale and industrial scale) of dyes and stains. This user-friendly handbook also features references on safety, toxicity, and adverse effects of dyes and stains on humans, animals, and the environment, including: acute/chronic toxicity aquatic toxicity carcinogenicity cytotoxicity ecotoxicity genotoxicity hepatotoxicity marine toxicity mutagenicity nephrotoxicity neurotoxicity oral toxicity phototoxicity phytotoxicity The use of biological dyes and stains has extremely high potential in today's business environment. This makes Handbook of Biological Dyes and Stains a convenient, must-have reference. Its staining, biological, and industrial applications make it a vital resource for industrial and academic researchers; the book also serves as a valuable desktop reference for medical professionals, biologists, chemists, chemical/optical engineers, physicists, materials scientists, intellectual property professionals, students, and professors.

The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes.The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research.TOPICS COVERED:* Single and multiphoton confocal microscopy* Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies* Applications of new optical probes for imaging proteins in gels * Probes and detection technologies for imaging membrane potential in live cells* Use of optical probes to detect microorganisms* Raman and confocal raman microspectroscopy* Fluorescence lifetime imaging microscopy* Digital CCD cameras and their application in biological microscopy

THE twisting and weaving of Nature's materials, grasses, twigs, rushes and vines into useful and beautiful forms seems almost instinctive in man. Perhaps it came to him as the nest-weaving instinct comes to birds - for at first he used it as they do, in the building of his house. Later shields and boats were formed of wicker work but how long ago the first basket was made no one is wise enough to tell us. Today Indian tribes in South America weave baskets from their native palms, South African negroes use reeds and roots, while the Chinese and Japanese are wonderful workmen in this as in other arts and industries; but basketry has come down to us more directly through the American Indian...