The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS

The practical aspects of flow cytometry and sorting are emphasized in this book which introduces the beginner to the technology and provides tips and tricks for the advanced user. The clear structure makes it easy to address specific problems fast. The chapters cover the modern applications of these procedures, with emphasis on immunofluorescence (antibody-fluorochrome conjugation, staining principles and data evaluation); the isolation of specific chromosomes, cells and fragile, large particles by magnetic and fluorescence-activated sorting; cellular biochemistry; and the dynamics of proliferation. The methods have been field-tested in recent EMBO courses on flow cytometry.

Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.

From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.

Revised and updated, this Second Edition of a classic text describes and evaluates--in greater detail--the most recent practical applications of flow cytometry technique to basic cellular biological investigations and clinical research on human neoplasms. Ideal for the experienced researcher as well as the novice, this informative book offers state-of-the-art reviews of all aspects of flow cytometry. New articles highlight investigations of higher plants, the flow cytometry of microorganisms, and measurements of intracellular ionized calcium and membrane potential--illustrating techniques of specimen preparation, measurement and analysis for each. New chapters examine applications of flow cytometry to medical genetics, genetic toxicology, and ultrasensitive analysis of molecules in solution. The Second Edition goes beyond the traditional analysis of DNA histograms with BrdU incorporation and DNA denaturability to identify and analyze the cell cycle more precisely. New or rewritten chapters discuss the importance of flow cytometry for measurements of nucleic acids, chromatin, and DNA and cover the cytometry of sperm and the cytopathic effects of viruses.