Fleshy Fruits are a late acquisition of plant evolution. In addition of protecting the seeds, these specialized organs unique to plants were developed to promote seed dispersal via the contribution of frugivorous animals. Fruit development and ripening is a complex process and understanding the underlying genetic and molecular program is a very active field of research. Part of the ripening process is directed to build up quality traits such as color, texture and aroma that make the fruit attractive and palatable. As fruit consumers, humans have developed a time long interaction with fruits which contributed to make the fruit ripening attributes conform our needs and preferences. This issue of Frontiers in Plant Science is intended to cover the most recent advances in our understanding of different aspects of fleshy fruit biology, including the genetic, molecular and metabolic mechanisms associated to each of the fruit quality traits. It is also of prime importance to consider the effects of environmental cues, cultural practices and postharvest methods, and to decipher the mechanism by which they impact fruit quality traits. Most of our knowledge of fleshy fruit development, ripening and quality traits comes from work done in a reduced number of species that are not only of economic importance but can also benefit from a number of genetic and genomic tools available to their specific research communities. For instance, working with tomato and grape offers several advantages since the genome sequences of these two fleshy fruit species have been deciphered and a wide range of biological and genetic resources have been developed. Ripening mutants are available for tomato which constitutes the main model system for fruit functional genomics. In addition, tomato is used as a reference species for climacteric fruit which ripening is controlled by the phytohormone ethylene. Likewise, grape is a reference species for non-climacteric fruit even though no single master switches controlling ripening initiation have been uncovered yet. In the last period, the genome sequence of an increased number of fruit crop species became available which creates a suitable situation for research communities around crops to get organized and information to be shared through public repositories. On the other hand, the availability of genome-wide expression profiling technologies has enabled an easier study of global transcriptional changes in fruit species where the sequenced genome is not yet available. In this issue authors will present recent progress including original data as well as authoritative reviews on our understanding of fleshy fruit biology focusing on tomato and grape as model species.

Fleshy Fruits are a late acquisition of plant evolution. In addition of protecting the seeds, these specialized organs unique to plants were developed to promote seed dispersal via the contribution of frugivorous animals. Fruit development and ripening is a complex process and understanding the underlying genetic and molecular program is a very active field of research. Part of the ripening process is directed to build up quality traits such as color, texture and aroma that make the fruit attractive and palatable. As fruit consumers, humans have developed a time long interaction with fruits which contributed to make the fruit ripening attributes conform our needs and preferences. This issue of Frontiers in Plant Science is intended to cover the most recent advances in our understanding of different aspects of fleshy fruit biology, including the genetic, molecular and metabolic mechanisms associated to each of the fruit quality traits. It is also of prime importance to consider the effects of environmental cues, cultural practices and postharvest methods, and to decipher the mechanism by which they impact fruit quality traits. Most of our knowledge of fleshy fruit development, ripening and quality traits comes from work done in a reduced number of species that are not only of economic importance but can also benefit from a number of genetic and genomic tools available to their specific research communities. For instance, working with tomato and grape offers several advantages since the genome sequences of these two fleshy fruit species have been deciphered and a wide range of biological and genetic resources have been developed. Ripening mutants are available for tomato which constitutes the main model system for fruit functional genomics. In addition, tomato is used as a reference species for climacteric fruit which ripening is controlled by the phytohormone ethylene. Likewise, grape is a reference species for non-climacteric fruit even though no single master switches controlling ripening initiation have been uncovered yet. In the last period, the genome sequence of an increased number of fruit crop species became available which creates a suitable situation for research communities around crops to get organized and information to be shared through public repositories. On the other hand, the availability of genome-wide expression profiling technologies has enabled an easier study of global transcriptional changes in fruit species where the sequenced genome is not yet available. In this issue authors will present recent progress including original data as well as authoritative reviews on our understanding of fleshy fruit biology focusing on tomato and grape as model species.

Fruit ripening is an important aspect of fruit production. The timing of it affects supply chains and buying behaviour, and for consumers ripeness not only affects perceptions of health but has nutritional effects too. Ripeness is closely related to spoilage which has a major financial impact on agricultural industries. Currently there are fast moving developments in knowledge of the factors affecting fruit ripeness, and this up-to-date monograph seeks to draw together the disparate research in this area. The aim of the book is to produce a comprehensive account covering almost every area related to fruit ripening including the latest molecular mechanisms regulating fruit ripening, its impact on human nutrition and emerging research and technologies.

Fruits are an important part of the human diet as they provide the vitamins, minerals and phytochemicals that are essential for human health. Commercialization of the fruit has driven new frontiers for crop development. The study of model plant systems such as Arabidopsis and tomato has uncovered highly conserved complex regulatory networks involving MADS-box transcription factors for controlling critical aspects of flower and fruit development. In apple, the effects of suppressing the apple SEP1/2-like gene, MADS8, have shown that the function of MADS8 is likely involved in fruit development. Fruits of MADS8-suppressed apple lines had reduced fruit size due to a loss of flesh tissues and severely inhibited ripening. In this research, five independent MADS8-overexpressing (MADS8ox) apple lines were analyzed. It was found that changes in tree architecture when compared to wild-type were strongly correlated with MADS8-overexpression. Early in tree development, the overexpression of MADS8 was found to result in reprioritization of flowering over vegetative growth, thereby changing the overall architecture of the tree with detrimental effects observed in the following year. At the fruit level, MADS8 overexpression was found to increase fruit flesh development and accelerate ripening particularly as seen from an increased rate of degreening in MADS8ox fruits. The analysis of possible protein interactions with MADS8 showed the ability of MADS8 to homodimerize which suggests that this may be the stable or activating form of the MADS8 protein. Transformation of Arabidopsis thaliana for novel expression of MADS8 in floral tissues did not produce any significant change in floral phenotypes. Overall, the results from this research have provided clear support for MADS8 as a key genetic determinant in a both tree and fruit development in apple. Elucidation of the functions of MADS8 provides new opportunities for the development of apple lines with improved yield and fruit quality. The results from this research also adds to the growing body of work that shows the diversification of MADS-box protein functions in fruit development arising from a conserved genetic framework at floral development.

The Study of Plants in a Whole New Light “Matt Candeias succeeds in evoking the wonder of plants with wit and wisdom.” ―James T. Costa, PhD, executive director, Highlands Biological Station and author of Darwin's Backyard #1 New Release in Nature & Ecology, Plants, Botany, Horticulture, Trees, Biological Sciences, and Nature Writing & Essays In his debut book, internationally-recognized blogger and podcaster Matt Candeias celebrates the nature of plants and the extraordinary world of plant organisms. A botanist’s defense. Since his early days of plant restoration, this amateur plant scientist has been enchanted with flora and the greater environmental ecology of the planet. Now, he looks at the study of plants through the lens of his ever-growing houseplant collection. Using gardening, houseplants, and examples of plants around you, In Defense of Plants changes your relationship with the world from the comfort of your windowsill. The ruthless, horny, and wonderful nature of plants. Understand how plants evolve and live on Earth with a never-before-seen look into their daily drama. Inside, Candeias explores the incredible ways plants live, fight, have sex, and conquer new territory. Whether a blossoming botanist or a professional plant scientist, In Defense of Plants is for anyone who sees plants as more than just static backdrops to more charismatic life forms. In this easily accessible introduction to the incredible world of plants, you’ll find: • Fantastic botanical histories and plant symbolism • Passionate stories of flora diversity and scientific names of plant organisms • Personal tales of plantsman discovery through the study of plants If you enjoyed books like The Botany of Desire, What a Plant Knows, or The Soul of an Octopus, then you’ll love In Defense of Plants.

This book describes the strategy used for sequencing, assembling and annotating the tomato genome and presents the main characteristics of this sequence with a special focus on repeated sequences and the ancestral polyploidy events. It also includes the chloroplast and mitochondrial genomes. Tomato (Solanum lycopersicum) is a major crop plant as well as a model for fruit development, and the availability of the genome sequence has completely changed the paradigm of the species’ genetics and genomics. The book describes the numerous genetic and genomic resources available, the identified genes and quantitative trait locus (QTL) identified, as well as the strong synteny across Solanaceae species. Lastly, it discusses the consequences of the availability of a high-quality genome sequence of the cultivated species for the research community. It is a valuable resource for students and researchers interested in the genetics and genomics of tomato and Solanaceae.

Applied plant genomics and biotechnology reviews the recent advancements in the post-genomic era, discussing how different varieties respond to abiotic and biotic stresses, investigating epigenetic modifications and epigenetic memory through analysis of DNA methylation states, applicative uses of RNA silencing and RNA interference in plant physiology and in experimental transgenics, and plants modified to produce high-value pharmaceutical proteins. The book provides an overview of research advances in application of RNA silencing and RNA interference, through Virus-based transient gene expression systems, Virus induced gene complementation (VIGC), Virus induced gene silencing (Sir VIGS, Mr VIGS) Virus-based microRNA silencing (VbMS) and Virus-based RNA mobility assays (VRMA); RNA based vaccines and expression of virus proteins or RNA, and virus-like particles in plants, the potential of virus vaccines and therapeutics, and exploring plants as factories for useful products and pharmaceuticals are topics wholly deepened. The book reviews and discuss Plant Functional Genomic studies discussing the technologies supporting the genetic improvement of plants and the production of plant varieties more resistant to biotic and abiotic stresses. Several important crops are analysed providing a glimpse on the most up-to-date methods and topics of investigation. The book presents a review on current state of GMO, the cisgenesis-derived plants and novel plant products devoid of transgene elements, discuss their regulation and the production of desired traits such as resistance to viruses and disease also in fruit trees and wood trees with long vegetative periods. Several chapters cover aspects of plant physiology related to plant improvement: cytokinin metabolism and hormone signaling pathways are discussed in barley; PARP-domain proteins involved in Stress-Induced Morphogenetic Response, regulation of NAD signaling and ROS dependent synthesis of anthocyanins. Apple allergen isoforms and the various content in different varieties are discussed and approaches to reduce their presence. Euphorbiaceae, castor bean, cassava and Jathropa are discussed at genomic structure, their diseases and viruses, and methods of transformation. Rice genomics and agricultural traits are discussed, and biotechnology for engineering and improve rice varieties. Mango topics are presented with an overview of molecular methods for variety differentiation, and aspects of fruit improvement by traditional and biotechnology methods. Oilseed rape is presented, discussing the genetic diversity, quality traits, genetic maps, genomic selection and comparative genomics for improvement of varieties. Tomato studies are presented, with an overview on the knowledge of the regulatory networks involved in flowering, methods applied to study the tomato genome-wide DNA methylation, its regulation by small RNAs, microRNA-dependent control of transcription factors expression, the development and ripening processes in tomato, genomic studies and fruit modelling to establish fleshy fruit traits of interest; the gene reprogramming during fruit ripening, and the ethylene dependent and independent DNA methylation changes. - provides an overview on the ongoing projects and activities in the field of applied biotechnology - includes examples of different crops and applications to be exploited - reviews and discusses Plant Functional Genomic studies and the future developments in the field - explores the new technologies supporting the genetic improvement of plants